Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus)

Yuan, Qin; Lin, Shang; Fu, Ying-Shuang; Nie, Xi-Rui; Liu, Wen; Su, Yan; Han, Qiao-Hong; Zhao, Li; Zhang, Qing; Lin, Derong; Qin, Wen; Wu, Ding‐Tao

doi:10.1016/j.ijbiomac.2019.01.042

Cited by 213 publications

(158 citation statements)

References 55 publications

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“…The contents of polyphenolics in PPP-W and PPP-P were remarkably higher than those in other tested PPPs, which might be correlated with this fact that polyphenolics can be associated with polysaccharides and/or proteins more easily because of high temperature and high pressure during HWE and PWE extraction [32], as well as the degradation of PPPs during ultrasoundand microwave-assisted extraction [15]. Extraction methods can influence molecular weights, viscosities, and monosaccharide compositions of polysaccharides, which can also affect the bioactivities of polysaccharides [17,33]. Accordingly, M w , viscosities, and monosaccharide compositions of PPPs extracted by seven extraction methods were studied.…”

Section: Discussionmentioning

confidence: 89%

“…PWE was also performed by the formerly reported method with minor modifications [17]. Briefly, samples (10.0 g) were extracted by 100.0 mL of 80% (v/v) ethanol to remove small molecules.…”

Section: Pressurized Water Extractionmentioning

confidence: 99%

“…The ABTS, DPPH, and nitric oxide (NO) radical scavenging activities, and the ferric reducing antioxidant powers (FRAP) of PPPs were detected based on the formerly reported methods [16,17]. The positive control was Vitamin C or BHT.…”

Section: In Vitro Antioxidant Activitiesmentioning

confidence: 99%

“…Many studies have revealed that extraction techniques can influence the physicochemical structures and bioactivities of natural polysaccharides. For instance, the polysaccharides extracted by the MAE method exhibit stronger antioxidant abilities than those of other methods [19], the polysaccharides extracted by the PWE method possess better inhibition activities on α-glucosidase and α-amylase than those of other methods [17], and the molecular weight of polysaccharides obtained by the HWE process is higher than those of other methods [24]. Nevertheless, there is limited study on the influences of various extraction processes on the bioactivities and physicochemical properties of PPPs from natural resources [15].…”

Section: Introductionmentioning

confidence: 96%

“…However, it always possesses some defects, including long extraction time, high extraction temperature, and low extraction efficiency [16]. At present, several new and green extraction techniques have been carried out to prepare polysaccharides and PPPs, such as pressurized water extraction (PWE) [17,18], ultrasound-assisted extraction (UAE) [16,19], ultrasound-assisted enzymatic extraction (UAEE) [20,21], ultrasound-microwave-assisted extraction (UMAE) [22,23], microwave-assisted extraction (MAE) [24,25], and high-speed shearing homogenization extraction (HSHE) [26,27]. Many studies have revealed that extraction techniques can influence the physicochemical structures and bioactivities of natural polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

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Section: Discussionmentioning

confidence: 89%

“…PWE was also performed by the formerly reported method with minor modifications [17]. Briefly, samples (10.0 g) were extracted by 100.0 mL of 80% (v/v) ethanol to remove small molecules.…”

Section: Pressurized Water Extractionmentioning

confidence: 99%

Section: In Vitro Antioxidant Activitiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Quality evaluation of immunomodulatory polysaccharides from Agaricus bisporus by an integrated fingerprint technique

Yuan

Huang

et al. 2022

Food Frontiers

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Agaricus bisporus (A. bisporus) is a rich source of polysaccharides with medical and nutraceutical interests; however, the complex structure of A. bisporus polysaccharides (ABPs) poses a challenge to its quality control. This study aims to investigate the immunomodulatory fractions of ABPs and further establish a quality control system for the detection and characterization of bioactive ABPs. Two fractions of ABPs were obtained using gradient alcohol precipitation combined ultrafiltration, followed by characterization of molecular weight, monosaccharide composition, chemical structure, and evaluation of immunomodulatory activity. Results showed that the two isolated fractions with different molecular weight, 8.95 × 10 6 Da (ABP17-1) and 1.73 × 10 4 Da (ABP17-2), were more homogeneous and exerted stronger immunomodulatory activity than that of ABPs. Furthermore, an integrated fingerprint technique including HPSEC-MALL-RID and HPLC was applied for the fingerprint analysis of ABPs from 17 batches of A. bisporus, which showed high similarity. Besides, two polysaccharide fractions, two monosaccharides (glucose and galactose), and the main cellulose hydrolysate glucose were identified as potential quality control markers of ABPs.Overall, this study could help better understand the structure-activity relationship of ABPs and serve as a guidance for the quality control of active ABPs and their related products.

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Ultrasound‐assisted extraction of tamarind xyloglucan: an effective approach to reduce the viscosity and improve the α‐amylase inhibition of xyloglucan

Jiang

Yang

et al. 2022

J Sci Food Agric

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BACKGROUND Water extraction (WE) is the classical extraction method for tamarind xyloglucan (XyG), but its low yield, high viscosity and poor dispersion in aqueous solution are not conducive to the industrial applications. To promote the industrial application of tamarind XyG, an ultrasonic‐assisted extraction (UAE) method for extracting low‐viscosity XyG from tamarind kernel powder was proposed. RESULTS The yield of UAE‐XyG was higher (502.33 ± 0.036 g kg−1) than that of WE‐XyG (163.43 ± 0.085 g kg−1). UAE reduced the molecular weight, monosaccharide content and apparent viscosity of XyG. The hypoglycemic experiment in vitro showed that UAE‐XyG had a stronger inhibitory effect on α‐amylase activity than WE‐XyG, but its glucose dialysis retardation index was lower. CONCLUSION In sum, UAE is a type of extraction method that could effectively improve the yield of XyG and reduce its viscosity to expand its application without reducing its physiological activity. UAE exhibits an excellent potential in the extraction of XyG. © 2022 Society of Chemical Industry.

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Effects of extraction methods on the physicochemical characteristics and biological activities of polysaccharides from okra (Abelmoschus esculentus)

Cited by 213 publications

References 55 publications

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Quality evaluation of immunomodulatory polysaccharides from Agaricus bisporus by an integrated fingerprint technique

Ultrasound‐assisted extraction of tamarind xyloglucan: an effective approach to reduce the viscosity and improve the α‐amylase inhibition of xyloglucan

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