Effects of extraction method and storage of dry tissue on marine lipids and fatty acids

Sardenne, Fany; Bodin, Nathalie; Métral, Luisa; Crottier, Anaïs; Grand, Fabienne Le; Bideau, Antoine; Brisset, Blandine; Bourjea, Jérôme; Saraux, Claire; Bonhommeau, Sylvain; Kerzérho, Vincent; Bernard, Serge; Rouyer, Tristan

doi:10.1016/j.aca.2018.11.012

Cited by 25 publications

(17 citation statements)

References 47 publications

(61 reference statements)

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“…Their PLs contain a large proportion of polyunsaturated fatty acids (PUFAs) in the chemical structure and are particularly susceptible to rancidity and oxidation to affect the product quality (Legako, Dinh, Miller, & Brooks, ). But they are also the main lipolysis substrates for the generation of healthful free fatty acids, such as eicosapentaenoic acid (EPA, 20:5n‐3) and docosahexaenoic acid (DHA, 22:6n‐3) (Sardenne et al, ; Simopoulos, ). Therefore, it is worth to quantitatively analysis the PLs profile of these two fish and to investigate their change during the processing.…”

Section: Introductionmentioning

confidence: 99%

Composition and viriation of the polar lipids in round scad ( Decapterus maruadsi ) and hairtail ( Trichiurus lepturus ) during salt‐drying

Yang

Cai

et al. 2019

J Food Process Preserv

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Twelve categories of polar lipids (PLs) were quantified in round scad and hairtail during salt‐drying with electrospray ionization‐tandem mass spectrometry based on lipidomics. The major species were phosphatidylcholine (PC), phosphatidylethanolamine (PE), and ether‐linked PC (ePC), accounting for 67.8%, 11.1%, and 9.7% of total PLs in the raw round scad respectively, and 74.3%, 7.3%, and 10.9% in raw hairtail. Among the 272 and 263 kinds of PLs molecules detected in the two raw fish respectively, only 125 and 94 PLs were strongly affected by salt‐drying, including 28 PCs, 23 PEs, 21 ePCs, 9 phosphatidylinositols (PIs), 14 phosphatidylserines/phosphatidic acids/phosphatidylglycerols (PSs/PAs/PGs), 12 lysophosphatidylcholines (LPCs), 5 lysophosphatidylethanolamines (LPEs), 9 ether‐linked PEs (ePEs), 4 sphingomyelins (SMs) in round scad, and 21 PCs, 17 PEs, 15 ePCs, 10 PIs, 7 PSs/PAs/PGs, 9 LPCs, 5 LPEs, 6 ePEs, 4 SMs in hairtail. Salt‐drying had a very different effect on the two fish. Ultimately, the total PLs content decreased slightly (p > .01) in salt‐dried round scad product while increased significantly in hairtail final product (p < .01). Practical applications The change of PLs caused by salt‐drying is closely related to the flavor and quality of fish products, and is very important for fish preservation and processing.

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Section: Introductionmentioning

confidence: 99%

Composition and viriation of the polar lipids in round scad ( Decapterus maruadsi ) and hairtail ( Trichiurus lepturus ) during salt‐drying

Yang

Cai

et al. 2019

J Food Process Preserv

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“…Drying has been only used as a method for collecting lipids economically in the marine (fish) and biofuel (algae) industries. 11,12 We applied this method to remove lipids effectively, and as a result, amounts of lipids exuded from the tissue during the drying step. In detailed evaluation for lipid removal efficiency, the dried tissue showed rapid and complete lipid elimination, while the wet tissue revealed remaining lipids after a 60 min delipidation period.…”

Section: Discussionmentioning

confidence: 99%

Optimization of extracellular matrix extraction from human perirenal adipose tissue

Chun

Lee

et al. 2021

J Biomater Appl

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Human adipose tissue includes useful substrates for regenerative medicine such as the extracellular matrix (ECM), but most perirenal fat tissue is wasted after kidney surgery. Since a lot of adipose tissue can be procured after a kidney, we extracted ECM from human perirenal adipose tissue and optimized the extraction process. To verify the efficacy for ECM extraction, we compared the products in several steps. Perirenal adipose tissue was either finely homogenized or underwent crude manual dissection. The amount of extracted ECM was quantified with ELISA for verification of the initial tissue downsizing effect. To validate the drying effect for fast and complete delipidation, tissues were prepared in a dry or wet phase, and residual lipids were visualized with Oil-Red-O staining. The extracted lipid was assayed at each time point to quantify the appropriate delipidation time. To select the optimal decellularization method, tissues were treated with physical, chemical, or enzymatic method, and the residual cell debris were identified with histological staining. The biochemical properties of the ECM extracted by the above methods were analyzed. The ECM extracted by fine homogenization showed a significantly enhanced amount of collagen, laminin and fibronectin compared to the crude dissection method. The dried tissue showed fast and complete lipid elimination compared to the wet tissue. Complete delipidation was achieved at 45 min after acetone treatment. Additionally, 1% triton X-100 chemical treatment showed complete decellularization with well-preserved collagen fibers. Biochemical analysis revealed preserved ECM proteins, a high cell proliferation rate and normal cell morphology without cell debris or lipids. The established process of homogenization, drying, delipidation with acetone, and decellularization with Triton X-100 treatment can be an optimal method for ECM extraction from human perirenal adipose tissue. Using this technique, human perirenal adipose tissue may be a valuable source for tissue engineering and regenerative medicine.

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“…61 According Folch et al, 56 the only advantage of using the Bligh and Dyer method 50 is the reduction in the solvent/sample ratio; 1 part tissue to 3 parts in the chloroform/methanol method instead of 1 part tissue to 20 parts chloroform/methanol. This procedure has been widely used 62,63 ; Bell et al, 64 extractedlipids from Atlantics almon (Salmo salar) using the procedure described by Folch, 56 like wise Gigliotti et al, 65 reported on lipid extraction from the Atlantic Krill (Euphausia superba), Ramanathan and Das, 66 also extracted lipids from Scomberomorus commerson using the method described to study the oxidation of lipids from ground fish in the presence of antioxidants. This method has also been used for the extraction of lipids from Atlantic mackerel (Scombers combrus), 67 in the shark species Eusphyra blochii and Carcharhinus bleekeri.…”

Section: Folch Methodsmentioning

confidence: 99%

Oil extraction and derivatization method: a review

Quero-Jiménez¹,

Felipe²,

López³

2020

OAJS

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The objective of this work is to analyze and present the main methods of oil extraction. The present study suggests that different methods could be used to extract oil for food and feed purposes. The method to be used for the extraction depends on several factors, among which its cost and the materials to be used stand out. This work has reviewed wellknown and widely practiced methods of oil extraction namely and conventional methods (solvent extraction), as well as new innovative methods aimed at raising and optimizing oil yield and improving oil quality. The main derivatization methods are also reviewed since among edible oils the determination of fatty acids is one of the quality parameters most studied and disseminated in the scientific literature. Major shortcomings associated with the conventional methods are solvent consumption, extraction time lag and adverse thermal effects at high temperatures that can produce oxidative processes of lipids. New techniques such as microwave-assisted extraction, ultrasonic-assisted extraction, and supercritical fluid extraction have been developed, and are being used to effectively reduce these shortcomings. Although, as previously stated, the researchers apply the most feasible, least-cost method that suits their research purposes.

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Effects of extraction method and storage of dry tissue on marine lipids and fatty acids

Abstract: Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.

Cited by 25 publications

References 47 publications

Composition and viriation of the polar lipids in round scad ( Decapterus maruadsi ) and hairtail ( Trichiurus lepturus ) during salt‐drying

Composition and viriation of the polar lipids in round scad ( Decapterus maruadsi ) and hairtail ( Trichiurus lepturus ) during salt‐drying

Optimization of extracellular matrix extraction from human perirenal adipose tissue

Oil extraction and derivatization method: a review

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