Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates

Pawlaczyk, Izabela; Lewik-Tsirigotis, Marta; Capek, Peter; Matulovà, Maria; Sasinková, Vlasta; Dąbrowski, Paweł; Witkiewicz, Wojciech; Gancarz, Roman

doi:10.1016/j.carbpol.2012.10.011

Cited by 42 publications

(16 citation statements)

References 26 publications

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“…Its application of croton oil can induce significant inflammatory responses, as characterized by edema, neutrophil infiltration, prostaglandins production and increases in vascular permeability [29] . In summary, aqueous extracts of these medicinal plants present a potent anti-inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts from Moroccan thyme varieties

Khouya

Ramchoun

Hmidani

et al. 2015

Asian Pacific Journal of Tropical Biomedicine

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A B S T R A C TObjective: To evaluate the anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts of thyme varieties from Moroccan. Methods: The aqueous extracts of tree medicinal plants [Thymus atlanticus (T. atlanticus), Thymus satureioides and Thymus zygis (T. zygis)] were screened for their antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical-scavenging, ferric reducing antioxidant power assay, radical scavenging activity method, the inhibition of 2,2'-azobis (2-amidinopropane) dihydrochloride that induces oxidative erythrocyte hemolysis and thiobarbituric acid reactive substances assay. The anti-inflammatory activity of aqueous extracts was evaluated in vivo using croton oil-induced ear edema and carrageenan-induced paw edema in mice and rats, respectively. This extracts were evaluated in vitro for their anticoagulant activity at the different concentrations by partial thromboplastin time and prothrombin time activated. Results: All thyme varieties were found to possess considerable antioxidant activity and potent anti-inflammatory activity in the croton oil-induced edema. Administration of aqueous extracts of two varieties (50 mg/kg) (T. zygis and T. atlanticus) reduced significantly the carrageenan-induced paw edema similar to non-steroidal anti-inflammatory drug (indomethacin, 10 mg/kg). In partial thromboplastin time and prothrombin time tests, T. atlanticus and T. zygis extracts showed the strongest anticoagulant activity. In contrast, Thymus satureioides did not show the anticoagulant activity in these tests. Conclusions: All aqueous extracts possess considerable antioxidant activity and are rich in total polyphenol and flavonoid but they act differently in the process of inflammatory and coagulation studied. This study shows great variability of biological activities in thyme varieties.

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Section: Discussionmentioning

confidence: 99%

“…Well-designed prospective studies are needed to prove this hypothesis. These anti-inflammatory and anticoagulant effects have been mostly attributed to the polyphenol and flavonoid compounds found in large quantities in these plants [29,34]. Values are expressed as mean ± SEM of 6 measurements.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts from Moroccan thyme varieties

Khouya

Ramchoun

Hmidani

et al. 2015

Asian Pacific Journal of Tropical Biomedicine

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“…According to that work, the inhibitory effect probably was dependent on the carbohydrate part of the complex and the effect on thrombin was mediated by heparin cofactor II. However, it was proven following the example of similar polyphenolic–polysaccharide glycoconjugates isolated from Fragaria vesca L. leaves (Pawlaczyk et al , 2013) that if the glycoconjugate was richer in polyphenolic components, the in vitro anticoagulant effect was better. Inhibition of thrombin amidolytic activity by pomegranate fruit and grape seeds components was also reported (Cuccioloni et al ., 2009b).…”

Section: Discussionmentioning

confidence: 99%

Thrombin inhibitory activity of some polyphenolic compounds

et al. 2013

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Thrombin, also known as an active plasma coagulation factor II, belongs to the family of serine proteases and plays a crucial role in blood coagulation process. The process of thrombin generation is the central event of the hemostatic process and regulates blood coagulant activity. For this reason, thrombin inhibition is key to successful novel antithrombotic pharmacotherapy. The aim of our present study was to examine the effects of the well-known polyphenolic compounds on the activity of thrombin, by characterization of its interaction with selected polyphenols using different biochemical methods and biosensor BIAcore analyses. Only six compounds, cyanidin, quercetin, silybin, cyanin, (+)-catechin and (−)-epicatechin, of all examined in this study polyphenols caused the inhibition of thrombin amidolytic activity. But only three of the six compounds (cyanidin, quercetin and silybin) changed thrombin proteolytic activity. BIAcore analyses demonstrated that cyanidin and quercetin caused a strong response in the interaction with immobilized thrombin, while cyanin and (−)-epicatechin induced a low response. Lineweaver–Burk curves show that used polyphenol aglycones act as competitive thrombin inhibitors. Our results suggest that polyphenolic compounds might be potential structural bases and source to find and project nature-based, safe, orally bioavailable direct thrombin inhibitors.

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“…Strawberry ( Fragaria × ananassa Duch.) is renown as a marketable fruit due to its pleasant taste and flavour [ 74 ], and its high content of health-beneficial polyphenolic compounds [ 75 , 76 , 77 ]. Also other plant parts of different Fragaria species were shown to contain high levels of phenolic structures as studied in flowers [ 78 ], leaves [ 79 , 80 ] and roots [ 81 , 82 ].…”

Section: Case Studies—gc-ms Profiling Of Plant Samplesmentioning

confidence: 99%

Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling

Rohloff

2015

Molecules

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Metabolite profiling has been established as a modern technology platform for the description of complex chemical matrices and compound identification in biological samples. Gas chromatography coupled with mass spectrometry (GC-MS) in particular is a fast and accurate method widely applied in diagnostics, functional genomics and for screening purposes. Following solvent extraction and derivatization, hundreds of metabolites from different chemical groups can be characterized in one analytical run. Besides sugars, acids, and polyols, diverse phenolic and other cyclic metabolites can be efficiently detected by metabolite profiling. The review describes own results from plant research to exemplify the applicability of GC-MS profiling and concurrent detection and identification of phenolics and other cyclic structures.

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Effects of extraction condition on structural features and anticoagulant activity of F. vesca L. conjugates

Cited by 42 publications

References 26 publications

Anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts from Moroccan thyme varieties

Anti-inflammatory, anticoagulant and antioxidant effects of aqueous extracts from Moroccan thyme varieties

Thrombin inhibitory activity of some polyphenolic compounds

Analysis of Phenolic and Cyclic Compounds in Plants Using Derivatization Techniques in Combination with GC-MS-Based Metabolite Profiling

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