Effects of Extract and Phenol Glycoside from Rose Petals on the Amylin Fibrils

Abstract

Peptide amyloidoses are considered as causes of a variety of pathologies (Alzheimer's disease, Parkinson's disease, type 2 diabetes, etc.). In the present work, the results of the transmission and scanning electron microscopy (TEM and SEM) were used to study the effects of the ethanol extract of rose petals and phenol glycoside fraction, isolated from it, on the fibrillation of the amyloid polypeptide amylin (AIAPP), which is toxic for islet cells. At TEM and SEM visualization of amylin fibrils, the size, form-factor, distribution by dimension and by polymorphism degree were taken into account. The nature of conformational diversity of aggregates of varying degree was analyzed. The analyses showed simultaneous presence of various structural forms: protofibrils, mature fibrils and ribbon-like forms. In case of plant preparations, a) inclusions of their particles caused increase of fibril dimention; b) amorphous bundles without clear configuration of the structure appeared, etc. These observations are in concordance with the earlier observed ability of these plant preparations to hinder the amylin fibrillation and to protect the islet cells from the toxicity of aggregated amylin. The findings of the present work demonstrate TEM and SEM as reasonable approaches in seeking effective antiamyloidogenic agents.

Mitigation with plant ethanol extracts of STZ-induced histopathological injuries in the tissues of laboratory rats

“…The in vitro and in vivo effects of ethanol extracts from several plants and their constituents on the activities of these enzymes were described [17]. The transmission and scanning electron microscopy study proved the ability of the ethanol extract from rose petals and of its phenole glycoside fraction to hinder the aggregation of the pancreas peptide hormone, amylin, which is one of the causes of developing DM [18]…”