Effects of extracellular magnesium extract on the proliferation and differentiation of human osteoblasts and osteoclasts in coculture

Wu, Limin; Feyerabend, Frank; Schilling, Arndt F.; Willumeit‐Römer, Regine; Luthringer, Bérengère

doi:10.1016/j.actbio.2015.08.042

Cited by 168 publications

(140 citation statements)

References 42 publications

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“…These findings are consistent with those reported by others and are in support of further investigating this novel material in musculoskeletal research [25,26,31].…”

Section: Discussionsupporting

confidence: 83%

“…Early mineralization of the callus was enhanced and then continued normally. Furthermore, the Mg2Ag alloy reduced the number and activity of osteoclasts, a finding that is consistent with our in vitro observations and with the results reported by others [26,31,35]. Thus, the increased callus formation mediated by intramedullary Mg2Ag implants during fracture healing in mice is probably due to both an increase in bone formation and a decrease in bone resorption.…”

Section: Discussionsupporting

confidence: 82%

“…Thus, the increased callus formation mediated by intramedullary Mg2Ag implants during fracture healing in mice is probably due to both an increase in bone formation and a decrease in bone resorption. These findings therefore strongly suggest the suitability of degradable Mg2Ag implants to promote long bone fracture healing in mice and are consistent with studies reporting positive effects of Mg implants on osteoblasts and bone formation [12][13][14]31,36,37]. However, we also noticed that Mg2Ag implants caused alterations of the shape of long bones under non-fracture and fracture conditions.…”

Section: Discussionsupporting

confidence: 79%

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Intramedullary Mg2Ag nails augment callus formation during fracture healing in mice

Jähn¹,

Saito

Taipaleenmäki

et al. 2016

Acta Biomaterialia

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Intramedullary stabilization is frequently used to treat long bone fractures. Implants usually remain unless complications arise. Since implant removal can become technically very challenging with the potential to cause further tissue damage, biodegradable materials are emerging as alternative options. Magnesium (Mg)-based biodegradable implants have a controllable degradation rate and good tissue compatibility, which makes them attractive for musculoskeletal research. Here we report for the first time the implantation of intramedullary nails made of an Mg alloy containing 2% silver (Mg2Ag) into intact and fractured femora of mice. Prior in vitro analyses revealed an inhibitory effect of Mg2Ag degradation products on osteoclast differentiation and function with no impair of osteoblast function. In vivo, Mg2Ag implants degraded under non-fracture and fracture conditions within 210 days and 133 days, respectively. During fracture repair, osteoblast function and subsequent bone formation were enhanced, while osteoclast activity and bone resorption were decreased, leading to an augmented callus formation. We observed a widening of the femoral shaft under steady state and regenerating conditions, which was at least in part due to an uncoupled bone remodeling. However, Mg2Ag implants did not cause any systemic adverse effects. These data suggest that Mg2Ag implants might be promising for intramedullary fixation of long bone fractures, a novel concept that has to be further investigated in future studies.

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“…These findings are consistent with those reported by others and are in support of further investigating this novel material in musculoskeletal research [25,26,31].…”

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Intramedullary Mg2Ag nails augment callus formation during fracture healing in mice

Jähn¹,

Saito

Taipaleenmäki

et al. 2016

Acta Biomaterialia

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“…About half of the total amount of Mg in our body is stored in bone tissue [24]. Wu et al reported that Mg ions (Mg 2+ ) govern a stimulating effect on the new bone formation by increasing the proliferation and differentiation of osteogenic (stem) cells via osteogenesis-related signaling pathways in vitro [25]. The release of Mg 2+ is related to the chemical reaction of Mg in the human body fluids based on the following reaction which produces Mg hydroxide (Mg(OH) 2 ) and hydrogen gas (H 2 ) evolution [26]:…”

Section: Advantages Of Mg and Its Alloysmentioning

confidence: 99%

Biodegradable Metallic Wires in Dental and Orthopedic Applications: A Review

Asgari

Hang

Chang

et al. 2018

Metals

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Abstract:Owing to significant advantages of bioactivity and biodegradability, biodegradable metallic materials such as magnesium, iron, and zinc and their alloys have been widely studied over recent years. Metallic wires with superior tensile strength and proper ductility can be fabricated by a traditional metalworking process (drawing). Drawn biodegradable metallic wires are popular biodegradable materials, which are promising in different clinical applications such as orthopedic fixation, surgical staples, cardiovascular stents, and aneurysm occlusion. This paper presents recent advances associated with the application of biodegradable metallic wires used in dental and orthopedic fields. Furthermore, the effects of some parameters such as the surface modification, alloying elements, and fabrication process affecting the degradation rate as well as biocompatibility, bioactivity, and mechanical stability are reviewed in the most recent works pertaining to these materials. Finally, possible pathways for future studies regarding the production of more efficient biodegradable metallic wires in the regeneration of bone defects are also proposed.

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“…Several studies have confirmed that the Mg and Si ions could stimulate osteoblast differentiation, and Si ions could also improve the ability of both osteoblasts and fibroblasts to produce collagen type I, which could promote cell differentiation. [47][48][49] Therefore, in this study, the increased ALP activity probably resulted from m-MS, indicating that the Mg and Si ions of m-MS might be responsible for stimulating cell differentiation. In conclusion, the WP40 composite with good cytocompatibility might have good biofunctions to stimulate bone repair and regeneration.…”

Section: Alp Activity Of Cells On Compositesmentioning

confidence: 99%

Influences of mesoporous magnesium silicate on the hydrophilicity, degradability, mineralization and primary cell response to a wheat protein based biocomposite

Feng

Jiang

et al. 2016

J. Mater. Chem. B

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A novel bioactive composite based on wheat protein (WP) and mesoporous magnesium silicate (m-MS) with a high specific surface area is presented in this study for potential bone tissue regeneration. Wheat protein (WP) is a type of a biodegradable natural polymer material. The m-MS was prepared by the sol-gel technique, which was incorporated into WP to fabricate m-MS/WP composites. The increasing amount of m-MS improved the surface hydrophilicity of m-MS/WP composites. The results showed that the degradation ratio of the m-MS/WP composites increased with an increase in the m-MS content after it was soaked in a Tris-HCl solution for 12 weeks. Moreover, the m-MS/WP composites with 40 wt% m-MS content (WP40) were able to maintain a suitable pH value over a prolonged soaking time, which might be dependent on the content of the m-MS. The WP40 showed a good apatite formation ability after it was soaked in simulated body fluid (SBF) for 7 days, indicating good bioactivity. Moreover, the WP40 with cytocompatibility stimulated the attachment, proliferation and differentiation of MC3T3-E1 osteoblast cells. Briefly, the results indicated that WP40 had good bioactivity, degradability, cytocompatibility and osteogenesis and might be a new biomaterial for bone regeneration.

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Effects of extracellular magnesium extract on the proliferation and differentiation of human osteoblasts and osteoclasts in coculture

Cited by 168 publications

References 42 publications

Intramedullary Mg2Ag nails augment callus formation during fracture healing in mice

Intramedullary Mg2Ag nails augment callus formation during fracture healing in mice

Biodegradable Metallic Wires in Dental and Orthopedic Applications: A Review

Influences of mesoporous magnesium silicate on the hydrophilicity, degradability, mineralization and primary cell response to a wheat protein based biocomposite

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