Effects of extenders and cryoprotectants on cryopreservation of Thai red junglefowl (Gallus gallus gallus) spermatozoa

Polsang, Sineenart; Thananurak, Pachara; Polsang, Pongtep; Inchaya, Suwit; Ponchunchoovong, Samorn; Chapanya, Jullachat; Kunhareang, Sajee; Vongpralub, Thevin

doi:10.1016/j.cryobiol.2022.04.004

Cited by 2 publications

(2 citation statements)

References 60 publications

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“…Increased production of ROS and depletion of natural antioxidative activity might have resulted in somewhat greater difference between sperm motility and viability through all stages of cryopreservation in control compared with ascorbic acid‐supplemented groups. Previous studies have also reported higher values of sperm motility compared with viability in red jungle fowl (Polsang et al, 2022; Rakha et al, 2017).…”

Section: Discussioncontrasting

confidence: 84%

Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen

Rakha

Ansari

Zuha

et al. 2023

Reprod Domestic Animals

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The Indian red jungle fowl population is decreasing in its natural habitat. Its conservation through semen cryopreservation with sufficient live sperm recovery rate is requisite where ascorbic acid could play significant role to mitigate cryo‐incited injuries. The objective was to elucidate the effect of ascorbic acid on freezability of Indian red jungle fowl sperm. Pooled semen was aliquoted and diluted (1:5) with red fowl extender having ascorbic acid: 0.0 (control), 1.0, 2.0 and 4.0 mM. Diluted samples were cryopreserved and semen quality was assessed at post‐dilution, cooling, equilibration and freeze–thawing stages. Sperm metabolic status, antioxidant potential and lipid peroxidation were studied at post‐dilution and freeze–thawing. Sperm motility did not differ (p > .05) in experimental extenders and control at post‐dilution and cooling; however, it was recorded higher (p < .05) with ascorbic acid at 2.0 mM compared with other levels at post‐equilibration and post‐thawing stage. Sperm viability, plasma membrane and acrosome intactness were recorded higher (p < .05) with 2.0 mM ascorbic acid compared with other concentrations of ascorbic acid at all stages of cryopreservation. Sperm metabolic status and antioxidant potential were recorded higher (p < .05), while lipid peroxidation was recorded lowest (p < .05) with 2.0 mM ascorbic acid compared with 1.0, 4.0 mM and control. In conclusion, ascorbic acid at 2.0 mM in red fowl extender improves quality, metabolic status and antioxidant potential of frozen Indian red jungle fowl semen through ameliorating lipid peroxidation.

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Section: Discussioncontrasting

confidence: 84%

Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen

Rakha

Ansari

Zuha

et al. 2023

Reprod Domestic Animals

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“…The mean semen volume of the 20-month-old Dang Surat rooster was 265±23 µl (Table 1). This accords with other Thai native roosters: 215±80 µl (range 80-500 µl) for Pradu-hangdum (Khunkaew et al, 2021); 282 ± 89 µl for one-year-old Thai red junglefowl (Polsang et al, 2022), 255 ± 90 µl 7-8-month old and 208 ± 60 µl for 10-11-month old Thai native roosters (Inyawilert et al, 2019). Semen volumes may be age dependent: Thai native rooster, 8-13 months of age were not significant but tended to be lower in younger roosters (Chotesangasa, 2001).…”

Section: Sperm Quality Assessmentsupporting

confidence: 85%

Semen characterization of Dang Surat Thai native chicken

Lewchalermvong

Kulnanan

Boonchay

et al. 2022

VIS

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Native Dang Surat chicken play an important role in poultry breeding of southern Thailand but their production performance falls short of commercial counterparts. Nevertheless, native birds have superior disease resistance while meat texture and taste of eggs are popular among Thais. Husbandry of native breeds are confined to smallholders who can only fulfil local niche outlets. To satisfy the wide-spread demand throughout Thailand and further afield, local-breed sires are crossed with high productivity hens. To maintain a constant supply of F1 hybrids, a colony of Dang Surat cocks are maintained by the Department of Livestock who select by phenotypes, not semen quality that determines male productivity. Accordingly, semen volume, pH, color and consistency, sperm motility, concentration, viability were measured. Morphology was also assessed by using eosin-nigrosin staining. Semen was slightly alkaline (pH, 8.0±0.1), mean volume=265±23 μL, had high sperm concentrations, 2740±76 x106/ml, of which 98.9±0.1% were viable. Abnormal sperm heads, tails and mid pieces, cytoplasmic droplets and detached heads comprised 1.5±0.3, 3.7±0.4, 0.3±0.1 and 0.8±0.1%, respectively. The findings showed normal seminal fluids and sperm parameters similar to commercial poultry semen in general. Our data will help develop efficient cockerel selection, natural flock breeding plan, and assisted reproduction by cryopreservation and artificial insemination to better meet market demands.

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Effects of extenders and cryoprotectants on cryopreservation of Thai red junglefowl (Gallus gallus gallus) spermatozoa

Cited by 2 publications

References 60 publications

Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen

Effect of ascorbic acid on metabolic status, lipid peroxidation, antioxidant activity and quality of frozen Indian red jungle fowl (Gallus gallus murghi) semen

Semen characterization of Dang Surat Thai native chicken

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