Effects of extended storage of whole blood before leucocyte depletion on coagulation factors in plasma

Abstract

Leucodepleted plasma originating from leucodepleted whole blood maintains a satisfactory level of coagulation factors, even after the storage of whole blood for 18 h at room temperature before filtration.

The effect of different methods of leucoreduction on plasma coagulation factors

“…This was in agreement with Gosselin and Dwyre (2015) [17], who studied the effect of freezing on coagulation testing and found significant differences between fresh and frozen tested plasma for prothrombin time, APTT, factors V and VIII and ATIII. Contrary to our results, Kretzschmar et al [16], reported prolongation in APTT postfiltration with a polyurethane whole blood filter as well as a decrease in factor VIII:C; however, this was only significant after plasma storage at room temperature for 18 h preprocessing.…”

“…This study investigated the effect of two different methods of leucofiltration (filtration by inline whole blood polyester filters vs. automated apheresis machine) on the extrinsic and intrinsic pathway clotting factors in terms of prothrombin time, INR and PTT as well as on clotting factors V and VIII. There was a significantly prolonged prothrombin time in leucodepleted plasma samples, yet in our opinion, this might not be clinically significant as the absolute clotting time difference was 0.5 s. Contrary to our results, Kretzschmar et al [16] documented shorter prothrombin time postfiltration. They attributed this decrease to the release of factor V from platelets during storage or filtration process.…”

The Effect of Holding Times of Whole Blood and Its Components During Processing on In Vitro and In Vivo Quality

“…Factor VIII dropped by 14% during the 8-hour ambient hold and dropped further to values that were about 26% lower than the initial values. Others confirmed these results for 18-hour-held whole blood [50] but also found a slight increase of C3a-desArg, indicative of complement activation with no clear cause; however, the authors suspected that the clinical relevance was low because it is quickly detoxified after release. Serrano et al [51] compared freshly processed PRPderived plasma clotting factors with overnight-held buffy coat-derived plasma and demonstrated that for an extended panel of clotting factors (14 in total), values for various plasma clotting factors from overnightheld whole blood fell between 84% and 130% of fresh.…”

“…The authors commented that the single statistically significant difference could be considered to be due to chance and of no clinical relevance. Moreover, the half-life (T- 50) was not different at 26.2 ± 2.0 days for red cells from whole blood held for 8 hours vs 26.3 ± 2.6 days for those from whole blood held for 24 hours. The red cells were processed according to the PRP method, and white cells were not removed.…”

“…Optimal when leukoreduction performed after 8-to 24-h room temperature hold (shown for buffy coat-derived platelets only) [7,[56][57][58][59][60][61] Plasma Factor VIII 10%-30% lower, but clinical consequence probably low [3,6,13,14,19,[47][48][49][50][51][52] Other clotting factors no effect [13,19,47,[49][50][51][52] Details are given in the text.…”