Effects of exposure to sublethal copper concentration on glyoxalase system enzymes in specimens of Scapharca inaequivalvis

Antognelli, Cinzia; Baldracchini, Francesca; Frosini, Roberta; Andrea, Piazzoli; Talesa, Vincenzo Nicola; Giovannini, Elvio

doi:10.1016/j.bse.2005.11.008

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…As FF and the other two related antibiotics, TA and CA, are also known to induce lipid peroxidation in animal species (Farombi 2001;Monari et al 2008;Ren et al 2014), which leads to the production of lipid freeradicals, hydroperoxides, and carbonyl compounds such as a-ketoaldehydes, an increase of GI activity, a scavenger of the previously mentioned toxic molecules, could be expected as well. Nevertheless, other different 2-oxoaldehydes from MG (acetol, L-3-deoxyxylosone, L-xylosone, D-glucosone) may probably encompass other enzymatic pathways such as aldose reductase (Vander Jagt et al 2001;Antognelli et al 2006). Most likely, the different profile of glyoxalases enzymes in fish exposed to FF might indicate a decreased triosephosphate production flux from glucose and the strengthening in levels of reduced glutathione shielding the antibioticmediated oxidative processes.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

et al. 2016

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Florfenicol (FF) is employed in fish farms to contest or prevent bacterial infections. However, this pharmaceutical may produce reactive oxygen species that may cause biochemical changes in antibiotic-treated fish. The aim of this study was to evaluate the effect of FF on Rainbow Trout Oncorhynchus mykiss treated for 10 d with 7.5 and 15 mg/kg FF followed by a withdrawal period of 5 d. Superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, glyoxalase I and glyoxalase II, total glutathione, lactic dehydrogenase, and alkaline phosphatase were investigated in the livers of treated and untreated fish. A general impairment of antioxidant enzymes and metabolic indicators was measured in FF-treated Rainbow Trout. Onset of oxidative damage may have occurred during the antibiotic treatment as a consequence of the effect of FF toxicity at mainly the highest dose. Nevertheless, the rise in levels of total glutathione and glutathione S-transferase even after the withdrawal period may shield the antibiotic-mediated oxidative processes. Received December 22, 2015; accepted May 26, 2016.

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Section: Discussionmentioning

confidence: 99%

Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

et al. 2016

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“…It also made large influence on the growth of plants marked by the shortened root length, fewer leaves, and decline in plants biomass [1]. In addition, the exposure at sublethal copper concentration would also influence the pathway of metabolic glyoxalases of Scapharca inaequivalvis [2]. Superfluous absorption of copper could affect the liver of goats [3].…”

Section: Introductionmentioning

confidence: 99%

Detection of copper (II) in aqueous solution by immobilized urease obtained from agro-waste: Optimization of process variables

Talat

2009

Biotechnol Bioproc E

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^Äëíê~Åí= Urease was used for estimation of Cu 2+ in aqueous solution. Process variables were optimized by Central Composite Design using MINITAB ® 15 software. Results predicted by the design were close to experimental value indicating suitability of the model. 3D surface plot and isoresponse contour plot were helpful in predicting results by performing limited set of experiments. Urease was extracted from discarded seeds of pumpkin to apparent homogeneity by heat fractionation and gel filtration. Homogeneous enzyme preparation was further immobilized in 3.5% alginate. Effect of Cu 2+ on the activity of soluble and immobilized enzyme was investigated. Enzyme inhibition was biphasic, irreversible, and non-competitive (Ki = 1.06 µM). © KSBB hÉóïçêÇëW=rêÉ~ëÉI=^äÖáå~íÉI=fããçÄáäáò~íáçåI=oÉëéçåëÉ=pìêÑ~ÅÉ=jÉíÜçÇçäçÖó

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Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper

et al. 2017

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There is an acknowledged need for in vitro fish intestinal model to help understand dietary exposure to chemicals in the aquatic environment. The presence and use of such models is however largely restrictive due to technical difficulties in the culturing of enterocytes in general and the availability of appropriate established cell lines in particular. In this study, the rainbow trout (Oncorhynchus mykiss) intestinal derived cell line (RTgutGC) was used as a surrogate for the “gut sac” method. To facilitate comparison, RTgutGC cells were grown as monolayers (double-seeded) on permeable Transwell supports leading to a two-compartment intestinal model consisting of polarised epithelium. This two-compartment model divides the system into an upper apical (lumen) and a lower basolateral (portal blood) compartment. In our studies, these cells stained weakly for mucosubstances, expressed the tight junction protein ZO-1 in addition to E-cadherin and revealed the presence of polarised epithelium in addition to microvilli protrusions. The cells also revealed a comparable transepithelial electrical resistance (TEER) to the in vivo situation. Importantly, the cell line tolerated apical saline (1:1 ratio) thus mimicking the intact organ to allow assessment of uptake of compounds across the intestine. Following an exposure over 72 h, our study demonstrated that the RTgutGC cell line under sub-lethal concentrations of copper sulphate (Cu) and modified saline solutions demonstrated uptake of the metal with saturation levels comparable to short term ex situ gut sac preparations. Gene expression analysis revealed no significant influence of pH or time on mRNA expression levels of key stress related genes (i.e. CYP3A, GST, mtA, Pgp and SOD) in the Transwell model. However, significant positive correlations were found between all genes investigated suggesting a co-operative relationship amongst the genes studied. When the outlined characteristics of the cell line are combined with the division of compartments, the RTgutGC double seeded model represents a potential animal replacement model for ecotoxicological studies. Overall, this model could be used to study the effects and predict aquatic gastrointestinal permeability of metals and other environmentally relevant contaminants in a cost effective and high throughput manner.

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Effects of exposure to sublethal copper concentration on glyoxalase system enzymes in specimens of Scapharca inaequivalvis

Cited by 7 publications

References 32 publications

Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

Assessment of Detoxifying Markers for Florfenicol in Rainbow Trout Liver

Detection of copper (II) in aqueous solution by immobilized urease obtained from agro-waste: Optimization of process variables

Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper

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