Effects of exogenous nitric oxide on growth, active oxygen species metabolism, and photosynthetic characteristics in cucumber seedlings under NaCl stress

Abstract

The study was conducted by means of nutrient solution culture to investigate the effects of exogenous nitric oxide (NO) on growth of cucumber seedlings, active oxygen species metabolism and photosynthetic characteristics in cucumber leaves under 50 mmol/L NaCl stress. The results showed that 10-400 µmol/L exogenous sodium nitroprusside (SNP), especially 100 µmol/L SNP, significantly alleviated the injury to seedlings and increased seedling growth. The activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and the contents of photosynthetic pigments and proline also increased under 50 mmol/L NaCl stress. Similarly, net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) also increased significantly. However, exogenous nitric oxide application markedly decreased membrane permeability, rate of O 2 · − production, the contents of malondialdehyde (MDA) and H 2 O 2 , and intercellular CO 2 concentration (Ci) under 50 mmol/L NaCl stress.

Protective role of foliar-applied nitric oxide in Triticum aestivum under saline stress

“…Nitric oxide has been reported to be actively involved as a signaling molecule in scavenging ROS by regulating/enhancing the activities of antioxidant enzymes like CAT, SOD, and POD (Wang et al, 2004). In our study, application of NO as foliar spray markedly increased the activities of SOD, POD, and CAT and total soluble proteins, which was in agreement with some previous studies on different crops such as cucumber (Fan et al, 2007), rice (Wang et al, 2004), and beans (Tuncz-Ozdemir et al, 2009) in which the activities of SOD, POD, and CAT were reported to be increased due to NO application. Nitric oxide-induced enhanced activity of CAT has been reported under various abiotic stresses (Hasanuzzaman et al, 2011).…”

Cadmium-induced structural disturbances in pea leaves are alleviated by nitric oxide

“…Treatment with sodium nitroprusside (SNP) as a donor of NO increases the rate of photosynthesis, chlorophyll content, transpiration rate, and stomatal conductance in cucumber seedlings (Fan et al, 2007). Lum et al (2005) reported that SNP decreases the amount of RuBisCo activity and the β-subunits of the RuBisCo subunit-binding protein in mung bean (Phaseolus aureus L.).…”

Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity

“…Several findings indicate an improvement in the performance of enzymatic and non-enzymatic antioxidant systems in salt-stressed plants treated with NO donors. Cucumber seedlings hydroponically grown in medium containing 50 mM NaCl and 100 µM sodium nitroprusside (SNP; NO + donor) showed higher activity of superoxide dismutase (SOD), catalase (CAT), peroxidase and ascorbate peroxidase (APX) when compared to cucumber solely treated with 50 mM NaCl (Fan et al 2007). As a result, cells from these seedlings presented lower membrane permeability and decreased levels of O 2 -, H 2 O 2 and lipid peroxides (Fan et al 2007).…”

“…Cucumber seedlings hydroponically grown in medium containing 50 mM NaCl and 100 µM sodium nitroprusside (SNP; NO + donor) showed higher activity of superoxide dismutase (SOD), catalase (CAT), peroxidase and ascorbate peroxidase (APX) when compared to cucumber solely treated with 50 mM NaCl (Fan et al 2007). As a result, cells from these seedlings presented lower membrane permeability and decreased levels of O 2 -, H 2 O 2 and lipid peroxides (Fan et al 2007). The NO was found to have dual role on SOD by positively modulating the FeSOD expression and negatively affecting Cu/ZnSOD one.…”

“…Aegiceras corniculatum Cucumis sativus (Fan et al 2007) Cynodon dactylon (Liu et al 2016) Glycine max (Simaei et al 2012) Gossypium hirsutum Helianthus annuus (Arora & Bhatla 2015;Kaur & Bhatla 2016) Hordeum vulgare (Li et al 2008) Nicotiana tabacum (da-Silva et al 2017) Solanum lycopersicum (Manai et al 2014) Spinacia oleracea (Du et al 2015) Triticum aestivum (Zheng et al 2008;Hasanuzzaman et al 2011) Increase of K + /Na + ratio…”