Effects of exogenous interleukin‐1β on primary sporocysts of Schistosoma mansoni (Trematoda) incubated with plasma and hemocytes from schistosome‐susceptible and resistant Biomphalaria glabrata (Gastropoda)

Abstract

Abstract. The cytokine interleukin‐1β (IL‐1β) mediates interactions of immune and inflammatory cells in mammals. Previous reports also have linked plasma (cell‐free hemolymph) levels of IL‐1β in the snail Biomphalaria glabrata to resistance against Schistosoma mansoni. In the present study, fluorescent probes were used to study larval schistosome and snail hemocyte viability during in vitro encounters. Hemolymph (plasma and hemocytes) from schistosome‐susceptible (M‐line) and resistant (13–16‐R1) B. glabrata was added to sporocysts of S. mansoni and the viability of hemocytes and parasites was assessed. Next, IL‐1β was added to sporocyst‐hemolymph samples, the viability of sporocysts and hemocytes determined and then compared to control assays. The number of live sporocysts present after incubation for 1 h with hemolymph from M‐line snails was significantly greater than the number seen when hemolymph from 13–16‐R1 snails was tested. Nearly all sporocysts survived the 1 h incubation with M‐line hemolymph, and most of the hemocytes attached to sporocysts were dead. In contrast, nearly all sporocysts were dead when hemolymph from 13–16‐R1 snails was tested, and most attached hemocytes were alive. Addition of IL‐1β to M‐line hemolymph resulted in a dramatic increase in sporocyst death. Addition of IL‐1β to 13–16‐R1 hemolymph produced a small but significant increase in the rate of sporocyst death. These results indicate that the concentration of IL‐1β present in hemolymph from B. glabrata is directly related to the ability of this snail to kill S. mansoni sporocysts in vitro.