Effects of Exogenous GA3 and DPC Treatments on Levels of Endogenous Hormone and Expression of Key Gibberellin Biosynthesis Pathway Genes During Stem Elongation in Sugarcane

Qiu, Lihang; Chen, Rongfa; Luo, Hanmin; Fan, Yanting; Huang, Xing; Liu, Junxian; Xiong, Fei; Zhou, Huiwen; Gan, Chong-Kun; Wu, Jianming; Li, Yang–Rui

doi:10.1007/s12355-019-00728-7

Cited by 18 publications

(17 citation statements)

References 33 publications

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“…Our results showed that on days 6 and days 12 following GA treatment, the height of GA-treated sugarcane was significantly higher than that of control plants. This was consistent with our previous studies (Qiu et al, 2019b). In addition, the average daily growth rate of sugarcane was significantly higher than the control plants, with approximately 2 times on day 3, and 3.5 times on both days 6 and 12.…”

Section: Discussionsupporting

confidence: 93%

“…Moreover, GA content in leaves significantly increased after GA spraying (Qiu et al, 2019b). We also found that the expression level of GA20 ox1 and gibberellin insensitive dwarf 1 (GID1) was increased on days 3 and 6, and then decreased on days 12 and 24 after GA3 treatment in sugarcane (Qiu et al, 2019b). These results indicated that GA stimulated the sugarcane internode elongation by increasing its endogenous levels and possibly regulating the expression of a multitude of growth and developmental genes.…”

Section: Introductionmentioning

confidence: 51%

“…Our earlier studies indicated that miRNA-mRNA pairs are involved in zeatin biosynthesis, nitrogen metabolism, and plant hormone signal transduction pathways, and are strongly associated with internode elongation and development in sugarcane (Qiu et al, 2019a). Moreover, GA content in leaves significantly increased after GA spraying (Qiu et al, 2019b). We also found that the expression level of GA20 ox1 and gibberellin insensitive dwarf 1 (GID1) was increased on days 3 and 6, and then decreased on days 12 and 24 after GA3 treatment in sugarcane (Qiu et al, 2019b).…”

Section: Introductionmentioning

confidence: 96%

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Enhanced Activity of Genes Associated With Photosynthesis, Phytohormone Metabolism and Cell Wall Synthesis Is Involved in Gibberellin-Mediated Sugarcane Internode Growth

et al. 2020

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Internode elongation is an important trait in sugarcane as it affects the sugarcane yield. Gibberellin (GA) is a key modulator of internode elongation in sugarcane. Understanding the gene expression features of GA-mediated internode elongation has both scientific and practical significance. This study aimed to examine the transcriptomic changes in the internode elongation of sugarcane following GA treatment. Eighteen cDNA libraries from the internode tissues on days of 0, 3, and 6 in control and GA treatment groups were sequenced and their gene expression were studied. RNAseq analysis revealed 1,338,723,248 reads and 70,821 unigenes from elongating internodes of sugarcane. Comparative studies discovered a large number of transcripts that were differentially expressed in GA-treated samples compared to the control. Further analysis revealed that the differentially expressed genes were enriched in the metabolic process, one-carbon compound transport, and single-organism process. Kyoto Encyclopedia of Genes and Genomes pathway annotation showed significant enrichment in photosynthesis and plant hormone signal transduction, indicating its involvement in internode elongation. The function analysis suggested that metabolic pathways and biosynthesis of secondary metabolites, plant hormones, and cell wall components were enriched in the internodes of the GA-treated plants. The hub genes were identified, with the function of cellulose synthesis. The results of this study provide a global view of mRNA changes during sugarcane internode elongation and extend our knowledge of the GA-mediated cellular processes involved in sugarcane stem growth.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 96%

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Enhanced Activity of Genes Associated With Photosynthesis, Phytohormone Metabolism and Cell Wall Synthesis Is Involved in Gibberellin-Mediated Sugarcane Internode Growth

et al. 2020

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“…Many previous studies also showed similar results in other crops, including Ginkgo [95], lodgepole pine [96], Triploid Loquat [97], Jatropha curcas [98] and crape myrtle [99]. However, some other studies indicated the induction of endogenous GAs biosynthesis and signal transduction by exogenous GA in other crops, such as tree peony [100], apple [101] and sugarcane [102]. Therefore, the effects of exogenous GA 3 on endogenous GA metabolisms vary in different crops and the mechanisms need to be further studied.…”

Section: Discussionsupporting

confidence: 52%

Gibberellins regulate lateral root development that is associated with auxin and cell wall metabolisms in cucumber

et al. 2022

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Background: Cucumber is an economically important crop cultivated worldwide. However, it is susceptible to various stresses due to weak and shallow root systems, limiting its productivity. Lateral roots (LRs) are critical for plant stress tolerance and productivity. Increasing evidence showed that gibberellins (GAs) play important roles, positively or negatively, in LR development in many plants. Therefore, it is of great importance for cucumber production to study the role of GAs in LR development.Results: The application of various concentrations of GA 3 and uniconazole, a GA biosynthesis inhibitor, on germinated cucumber seeds for 5 days showed that GAs regulated cucumber LR development in a concentration-dependent manner. 1 µM, 10 µM, 50 µM and 100 µM GA 3 signi cantly increased secondary root length, tertiary root number and length. It is worthy note that 50 µM GA 3 treatment showed strong effects in increasing root dry weight and the root/shoot dry weight ratio. Pairwise comparisons between GA 3 -treated roots and Control at 2, 3 and 5 days after germination (DAG) identi ed 417 down-regulated genes enriched for GA metabolism-related processes and 447 up-regulated genes enriched for cell wall metabolism-related processes in GA 3 -treated roots. Linear factorial modeling was also performed to systematically identify DEGs and a total of 3523 non-redundant DEGs were identi ed in our RNA-seq data. Of these, most of the genes involved in auxin and cell wall metabolisms were up-regulated in GA 3 -

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“…No straight sign was found that ABA, GA, and ethylene signaling act in response to significant sucrose accumulation in sugarcane stalks. Plant height was improved by application of GA on sugarcane leaves at elongation time (Qiu et al, 2019; Rai et al, 2017; Verma et al, 2017; Wu et al, 2010). The application of ethephon on leaves also improved cane yield and sucrose content in sugarcane (Abberton et al, 2016; Li et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic exploration of a high sucrose mutant in comparison with the low sucrose mother genotype in sugarcane during sugar accumulating stage

et al. 2021

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Sugarcane (Saccharum spp. hybrid) is a noteworthy crop in the world for sugar and bioenergy production. In the present study, transcriptomic analysis was steered for a high sucrose mutant Guixuan B9 (GXB9) and the low sucrose mother genotype B9 of sugarcane during sugar accumulating stage. A total of 112,170 unigenes were obtained, and 106,026 (94.52%) were annotated by using the available public databases nonredundant, Gene ontology, COG, Kyoto Encyclopedia of Genes and Genomes, Swiss‐Prot, and Pfam. Functional assignment analysis of unigenes exhibited that they were active in a diversity of metabolic pathways. The vital unigenes for sucrose metabolism and accumulation were relatively characterized in the immature internodes (int. 5, 6) and maturing internodes (int. 13, 14) of GXB9 in comparison with B9. The differentially expressed unigenes (DEGs) encoding sucrose phosphate synthase (SPS, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/4/1/14.html), sucrose synthase (SuSy, http://www.chem.qmul.ac.uk/iubmb/enzyme/EC2/4/1/13.html), and invertase (http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/26.html), which are the vital enzymes functioning in the biosynthesis and splitting of sucrose, in the internodal tissues were revealed by using Illumina Hiseq 2000 platform, and verified by quantitative reverse transcription‐polymerase chain reaction analysis. The DEGs encoding SPS proteins SPS1, SPS2, SPS4, and SPS5 were upregulated in the maturing internodes of GXB9 compared with B9. The DEGs encoding SuSy proteins were majorly upregulated in the immature internodes of GXB9, and those encoding the enzymes involved in trehalose synthesis were downregulated in GXB9 compared with B9. The results in this study have revealed the opportunities for future sugarcane improvement by concentrating on the DEGs linked to sucrose metabolic pathways by applying omics technologies.

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Effects of Exogenous GA3 and DPC Treatments on Levels of Endogenous Hormone and Expression of Key Gibberellin Biosynthesis Pathway Genes During Stem Elongation in Sugarcane

Cited by 18 publications

References 33 publications

Enhanced Activity of Genes Associated With Photosynthesis, Phytohormone Metabolism and Cell Wall Synthesis Is Involved in Gibberellin-Mediated Sugarcane Internode Growth

Enhanced Activity of Genes Associated With Photosynthesis, Phytohormone Metabolism and Cell Wall Synthesis Is Involved in Gibberellin-Mediated Sugarcane Internode Growth

Gibberellins regulate lateral root development that is associated with auxin and cell wall metabolisms in cucumber

Transcriptomic exploration of a high sucrose mutant in comparison with the low sucrose mother genotype in sugarcane during sugar accumulating stage

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