Effects of Exogenous Fibrolytic Enzyme Derived from Trichoderma reesei on Rumen Degradation Characteristics and Degradability of Low-Tannin Whole Plant Faba Bean Silage in Dairy Cows

Abstract

The objectives of this study were to (1) determine the effect of exogenous fibrolytic enzyme derived from Trichoderma reesei on dry matter (DM) and neutral detergent fibre (NDF) degradability of whole plant faba bean silage (Snowbird), (2) evaluate the effects of fibrolytic enzyme (FETR) on DM and NDF degradation kinetics of whole plant faba bean silage, and (3) compare the difference between in the vitro approach (DaisyII incubation method) and the in situ assay-biological approach (nylon bag technique) in the determination of degradability of dry matter (DMD) and neutral detergent fibre (NDFD). The fibrolytic enzyme from Trichoderma reesei was a mixture of xylanase and cellulase. The whole plant faba bean silage was treated with seven doses of fibrolytic enzyme, with 0 as a control and 0.25, 0.5, 0.75, 1, 1.25 and 1.5 mL of FETR/kg DM of silage. The results obtained from the in situ method show that fibrolytic enzyme cubically (p < 0.05) affected DMD and quadratically (p < 0.01) affected NDFD with increasing level of enzyme application. In vitro DM and NDF degradability were quadratically and cubically (p < 0.01) affected by the increasing dosage of enzyme. Correlation analysis between the in situ assay-biological approach and the In vitro DaisyII approach showed a strong correlation (r = 0.98, p < 0.01) on overall DMD and also a satisfactory relationship (r = 0.84, p < 0.01) was found on overall NDFD. The enzyme application showed a great impact on NDF rumen degradation kinetics by decreasing the undegradable fraction and increasing the potential degradable fraction and the effective degradable content of fiber. The washable (W) and potential degradation fraction (D) were linearly (p = 0.05) increased by the enzyme treatments. Therefore, the undegradable fraction was linearly decreased (p = 0.05) with increasing dosage of enzyme. Both bypass (BNDF) and effective degradable NDF (EDNDF) were cubically (p = 0.05) affected by fibrolytic enzyme. In conclusion, the exogenous fibrolytic enzyme derived from Trichoderma reesei highly impacted rumen degradation characteristics and degradability of whole plant faba bean silage and could be used to improve fibre digestion of whole plant faba silage in dairy cows.

EFFECT OF USING PROBIOTICS ON ARABIAN CAMELS (Camelus dromedarius) FEEDING: AN IN VITRO STUDY.

“…May be the presence of exogenous enzymes (cellulase + xylanase + alfa amylase + protease) in Zad group enhanced and increased the soluble fraction (a). The same effect was noted by Yang et al, (2022) on whole plant faba bean silage that treated with gradually levels of exogenous fibrolytic enzyme derived from Trichoderma reesei with concentrations from zero to 1.5 (ml/Kg) that resulted in increasing soluble fraction of DM from 25.10 to 29.24%, respectively. Using exogenous fibrolytic enzymes resulted in pre-digestion effect that increased the soluble fraction of DM (Pinos-Rodrıguez et al, 2008).…”

Effect of dietary inclusions of different types of Acacia mearnsii on milk performance and nutrient intake of dairy cows

“…Treating AMF with exogenous enzyme might improve their utilization by dairy cows as it has been previously shown that exogenous fibrolytic enzyme increase milk parameters ( Gado et al., 2009 ; Golder et al., 2019 ). Fibrolytic enzymes enhance DM digestibility ( Yang et al., 2022 ) by increasing DM, OM and CP, and decreasing NDF and ADL ( Kumar et al., 2021 ).…”

A Review on the Role of Exogenous Fibrolytic Enzymes in Ruminant Nutrition

“…In contrast, Giraldo et al [29] observed enhanced fibrolytic activity in ruminal fluid when sheep were administered EFE @ 12 g/d intra-ruminally. These variable responses by fibrolytic enzymes across experiments may be due to differences in the enzyme's activity,substrate specificity, internal rumen environment and mode of application [30].…”