Effects of Exogenous Electron Acceptors on Kinetic Characteristics of Prompt and Delayed Fluorescence in Atrazine Inhibited Thylakoid Membranes

Goltsev, V.; Traikov, L.; Hristov, Valeri

doi:10.1007/978-94-011-3953-3_906

Cited by 4 publications

(5 citation statements)

References 6 publications

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“…The emission, decaying in the submillisecond domain (L 1 ), is connected with the recombination of Z + P 680 Q A − Q B − , and this decayed in millisecond time domain (L 2 )—with the recombination of Z + P 680 Q A − Q B = . The lifetimes (τ 1 and τ 2 ) are determined by the probabilities of direct and back electron transport in these states (33). These assumptions were fitted well for interpretation of experiments done with modificators of PSII electron transport (33) or of thylakoid membrane fluidity (48), as well as for interpretation of temperature dependences of DF decay parameters (43).…”

Section: Resultsmentioning

confidence: 99%

“…The lifetimes (τ 1 and τ 2 ) are determined by the probabilities of direct and back electron transport in these states (33). These assumptions were fitted well for interpretation of experiments done with modificators of PSII electron transport (33) or of thylakoid membrane fluidity (48), as well as for interpretation of temperature dependences of DF decay parameters (43). The millisecond characteristic time (τ 2 ) increases approximately two‐fold during the first second of illumination (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…6). This increase observed at the beginning of the induction period is possibly connected with the photoinduced PQ pool reduction (33) because with the closure of PQ pool, one of the ways for disappearance of the Z + P 680 Q A − Q B = state (namely, dissociation of the PQ molecule from the Q B binding site) becomes blocked. After the first few seconds of illumination, τ 2 only slightly decreases.…”

Section: Resultsmentioning

confidence: 99%

“…In each phosphoroscope working cycle, one DF dark relaxation kinetic (of 100–120 points situated via the time interval of ∼42 μs) was available. It represents a sum of two fast kinetic components (τ 1 ∼ 200–900 μs and τ 2 ∼ 1.2–3.9 ms) and a “tail” of slow components (τ 3 > 20 ms) (33).…”

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confidence: 99%

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Advances on Photosystem II Investigation by Measurement of Delayed Chlorophyll Fluorescence by a Phosphoroscopic Method¶

Zaharieva¹,

Goltsev²

2007

Photochemistry and Photobiology

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A method for data acquisition based on recording of light signal from a conventional phophoroscope fluorometer with high-speed digitalization is proposed to extract more information from a delayed chlorophyll a fluorescence (DF) signal. During the signal processing from all points registered by the fluorometer, we obtain simultaneously a large number of induction curves of DF decaying at different time ranges. In addition, it is possible to register a series of dark relaxation kinetics of DF, recorded at different moments during the induction period or at different temperatures. This allows the evaluation of the contribution of DF kinetic components during the induction period or at different temperatures and the comparison between DF signals registered with different phophoroscopes. With the phosphoroscope system used in this study, we have shown that the contribution of the millisecond components (with lifetimes 0.6 and 2-4 ms) predominates during the first second of the induction period. After 1 s of illumination, the amplitudes of the 0.6 ms and 2-4 ms components and of the slower one (with lifetime more than 10 ms) become approximately equal. The change in lifetime of the different components during the induction and during gradual heating is also observed. It is shown that all registered DF kinetic components have different temperature dependences.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Advances on Photosystem II Investigation by Measurement of Delayed Chlorophyll Fluorescence by a Phosphoroscopic Method¶

Zaharieva¹,

Goltsev²

2007

Photochemistry and Photobiology

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“…For a complete account of the knowledge on prompt fluorescence, see Papageorgiou and Govindjee (2004). The luminescent properties of the photosynthetic apparatus and the surface electric charge of the thylakoid membrane have been used for the investigation of the in vivo response of the photosynthetic apparatus under different stress (Goltsev et al 1986(Goltsev et al , 1987(Goltsev et al , 1998Zaharieva et al 2001Zaharieva et al , 2003Lambrev and Goltsev 2001).…”

Section: Department Of Biophysics and Radiobiology At Sofia Universitymentioning

confidence: 98%

A brief history of the investigations on photosynthesis in Bulgaria

Zeinalov

2006

Photosynth Res

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Investigations on photosynthesis in Bulgaria started in 1941 with the doctoral thesis of Kyrill Popov (University of Münster, Germany). In 1965, at the Institute of Plant Physiology, in Sofia, Kyrill Popov guided the establishment of a special group of researchers on the biochemistry, biophysics and physiology of photosynthesis. In this paper, a brief description of research in photosynthesis in Bulgaria is presented.

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Delayed fluorescence in photosynthesis

et al. 2009

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Photosynthesis is a very efficient photochemical process. Nevertheless, plants emit some of the absorbed energy as light quanta. This luminescence is emitted, predominantly, by excited chlorophyll a molecules in the light-harvesting antenna, associated with Photosystem II (PS II) reaction centers. The emission that occurs before the utilization of the excitation energy in the primary photochemical reaction is called prompt fluorescence. Light emission can also be observed from repopulated excited chlorophylls as a result of recombination of the charge pairs. In this case, some time-dependent redox reactions occur before the excitation of the chlorophyll. This delays the light emission and provides the name for this phenomenon-delayed fluorescence (DF), or delayed light emission (DLE). The DF intensity is a decreasing polyphasic function of the time after illumination, which reflects the kinetics of electron transport reactions both on the (electron) donor and the (electron) acceptor sides of PS II. Two main experimental approaches are used for DF measurements: (a) recording of the DF decay in the dark after a single turnover flash or after continuous light excitation and (b) recording of the DF intensity during light adaptation of the photosynthesizing samples (induction curves), following a period of darkness. In this paper we review historical data on DF research and recent advances in the understanding of the relation between the delayed fluorescence and specific reactions in PS II. An experimental method for simultaneous recording of the induction transients of prompt and delayed chlorophyll fluorescence and decay curves of DF in the millisecond time domain is discussed.

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Effects of Exogenous Electron Acceptors on Kinetic Characteristics of Prompt and Delayed Fluorescence in Atrazine Inhibited Thylakoid Membranes

Cited by 4 publications

References 6 publications

Advances on Photosystem II Investigation by Measurement of Delayed Chlorophyll Fluorescence by a Phosphoroscopic Method¶

Advances on Photosystem II Investigation by Measurement of Delayed Chlorophyll Fluorescence by a Phosphoroscopic Method¶

A brief history of the investigations on photosynthesis in Bulgaria

Delayed fluorescence in photosynthesis

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