Effects of exogenous application of plant growth regulators on the development of ovule and subsequent embryo rescue of stenospermic grape (Vitis vinifera L.)

Breeding new seedless grape by means of in vitro embryo rescue

“…Thus, some grape breeders have strived to cultivate excellent seedless varieties by embryo rescue (Emershad and Ramming, 1984;Spiegle-Roy et al, 1985;Gray et al, 1990; Ponce In the same year, our group was also committed to creating new varieties of seedless grapes by the same technology, and we found some excellent characteristics of Chinese wild Vitis spp, such as fungal disease resistance, absence of the foxy flavor, and higher hybridization affinity with V. vinifer (He et al, 1991;He and Luo, 1994;Wang et al, 1995Wang et al, , 1998Tian et al, 2008;Tang et al, 2009). Thus, in our study, two representative Chinese wild Vitis spp, 'Beichun' and 'Shuangyou', were also used as pollen parents to hybridize with V. vinifera.…”

“…China is one of the centers of origin of Vitis species, with many disease-resistant resources (He et al, 1991;He and Luo, 1994;Wang et al, 1995Wang et al, , 1998Tian et al, 2008;Tang et al, 2009). These wild Vitis spp do not have a foxy flavor, a limiting factor for the use of many North American species in grape breeding programs (Alleweldt and Possingham, 1988).…”

“…These wild Vitis spp do not have a foxy flavor, a limiting factor for the use of many North American species in grape breeding programs (Alleweldt and Possingham, 1988). 'Beichun' and 'Shuangyou', two representatives of the wild type, are usually used as pollen parents because of their higher hybridization affinity with Vitis vinifera (Wang et al, 1995;Tian et al, 2008;Tang et al, 2009). Besides good resistance to chilling, moisture, and other environmental stresses (Wang et al, 1998), 'Beichun' also exhibits a significantly higher level of resistance to both downy mildew and anthracnose compared with its wild parent (Liu et al, 2002;Liu and Li, 2004).…”

“…Earlier in 1982, Ramming and Emershad first reported that stenospermic grapes could generate plants via ovule culture. Since then, this embryo rescue technique has been widely applied to embryo germination of seedless grape cultivars in cross-breeding programs (Emershad and Ramming, 1984;Spiegle-Roy et al, 1985;Gray et al, 1990;Ponce et al, 2000;Bharathy et al, 2005;Tian et al, 2008;Tang et al, 2009;Singh et al, 2011). Nowadays, in vitro embryo rescue in grape breeding projects assures breeding efficiency by curtailing 6 to 8 years in the development of seedless grape cultivars (Singh et al, 2011), and the adoption of seeded cultivars as female parents has been replaced by using stenospermic grapes as females directly.…”

Immature Embryo Rescue and <i>in Vitro</i> Development Evaluation of Intraspecific Hybrids from Brazilian Seedless Grapevine “Superior × Thompson” Clones

“…Reference [15] obtained the rate of germination of 12.67% after 28 days of culture. In an experiment with "Centennial Seedless" and "Thompson Seedless" grape varieties by [16], using culture media supplemented with benzylaminopurine, 11.9% of developed embryos to the variety 'Thompson Seedless' were observed. In our case, the values obtained were around 50% of germinated seedlings, demonstrating the good efficiency of this methodology, using 0.1 mg•L −1 indoleacetic acid.…”