Effects of exogenous application of CPPU, NAA and GA 4+7 on parthenocarpy and fruit quality in cucumber ( Cucumis sativus L.)

Qian, Chunlu; Ren, Nannan; Wang, Jingye; Xu, Qiang; Chen, Xuehao; Qi, Xiaohua

doi:10.1016/j.foodchem.2017.09.150

Cited by 84 publications

(57 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In grapes, GA 4 content increases during later fruit developmental stages (Giacomelli et al, 2013). Exogenous application of GA 4 promotes vegetative and reproductive growth in tomato (García-Hurtado et al, 2012) and cucumber (Qian et al, 2018). Furthermore, the affinity of GA 4 to bind GA INSENSITIVE DWARF1 (GID1), a GA signal receptor, is approximately 20 times greater than that of GA 3 in rice (Ueguchitanaka et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

Wang

Liu

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Fruit set and development occur following successful fertilization. Parthenocarpy, a valuable trait in some self-incompatible species, produces seedless fruit without fertilization. Gibberellin (GA) is a crucial hormone in fruit-set regulation and development. While investigating the development of parthenocarpy in pear (Pyrus bretschneideri Rehd.), we determined that GA 20-oxidases (GA20ox) may play key roles in seedless pear fruit development. Sequence analysis revealed three PbGA20ox genes: PbGA20ox1, PbGA20ox2, and PbGA20ox3. We analyzed the expression patterns of candidate genes and found that PbGA20ox2 levels significantly changed in pollinated fruits. Tissue-specific expression assays revealed that PbGA20ox2 is highly expressed in young fruit and leaves. Subcellular localization assays showed it was located in the cytoplasm, nucleus, and plasma membrane. Overexpressed PbGA20ox2 tomato plants were taller and had longer hypocotyls and internodes, and the emasculated flowers produced parthenocarpic fruit. In pear, the transient overexpression of PbGA20ox2 promoted fruit development and delayed the drop of nonpollinated fruit. Furthermore, the fruit of PbGA20ox2-overexpressing tomato and transient PbGA20ox2overexpressing pear had increased GA 4 (but not GA 3 and GA 1 ) contents. This result provided evidence that PbGA20ox2 was necessary for GA 4 -dependent pear fruit development. Our study revealed that PbGA20ox2 altered the GA biosynthetic pathway and enhanced GA 4 synthesis, thereby promoting fruit set and parthenocarpic fruit development.

show abstract

Section: Discussionmentioning

confidence: 99%

PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

Wang

Liu

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Cucumber ( Cucumis sativus L.) is an economically important crop cultivated worldwide [ 32 ] and has been used as a model plant for studying hormonal regulation of reproductive development [ 33 , 34 ]. Furthermore, GA was demonstrated to play an important role in flower and fruit development in cucumber [ 7 , 18 , 33 , 35 ]. In cucumber, the final part of the GA biosynthetic pathway is catalyzed by four subfamilies of GAoxs: GA 7-oxidases (GA7oxs), GA20oxs, GA3oxs and GA2oxs [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Cucumber Gibberellin Oxidase Family Genes and Functional Characterization of CsGA20ox1 in Root Development in Arabidopsis

Sun

Pang

Yan

et al. 2018

IJMS

View full text Add to dashboard Cite

Cucumber (Cucumis sativus L.) is an important vegetable crop worldwide and gibberellins (GAs) play important roles in the regulation of cucumber developmental and growth processes. GA oxidases (GAoxs), which are encoded by different gene subfamilies, are particularly important in regulating bioactive GA levels by catalyzing the later steps in the biosynthetic pathway. Although GAoxs are critical enzymes in GA synthesis pathway, little is known about GAox genes in cucumber, in particular about their evolutionary relationships, expression profiles and biological function. In this study, we identified 17 GAox genes in cucumber genome and classified them into five subfamilies based on a phylogenetic tree, gene structures, and conserved motifs. Synteny analysis indicated that the tandem duplication or segmental duplication events played a minor role in the expansion of cucumber GA2ox, GA3ox and GA7ox gene families. Comparative syntenic analysis combined with phylogenetic analysis provided deep insight into the phylogenetic relationships of CsGAox genes and suggested that protein homology CsGAox are closer to AtGAox than OsGAox. In addition, candidate transcription factors BBR/BPC (BARLEY B RECOMBINANT/BASIC PENTACYSTEINE) and GRAS (GIBBERELLIC ACID-INSENSITIVE, REPRESSOR of GAI, and SCARECROW) which may directly bind promoters of CsGAox genes were predicted. Expression profiles derived from transcriptome data indicated that some CsGAox genes, especially CsGA20ox1, are highly expressed in seedling roots and were down-regulated under GA3 treatment. Ectopic over-expression of CsGA20ox1 in Arabidopsis significantly increased primary root length and lateral root number. Taken together, comprehensive analysis of CsGAoxs would provide a basis for understanding the evolution and function of the CsGAox family.

show abstract

“…The constructed strain resulted in a 2.5-fold increase in GAs yield (Albermann et al, 2013). Compared to GA3, GA 4 , and GA 7 may perform more excellently when treating different plants (Kim and Miller, 2009;Curry, 2012;Qian et al, 2018). Tudzynski et al deleted the P450-3 gene and the resulted mutant could not produce GA 1 and GA 3 anymore, while the production of GA 4 and GA 7 was significantly increased (Tudzynski et al, 2003).…”

Section: Strain Improvement For Ga Productionmentioning

confidence: 99%

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

Cen

Lin

Wang

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

In recent years, there has been a noticeable increase in research interests on the Fusarium species, which includes prevalent plant pathogens and human pathogens, common microbial food contaminants and industrial microbes. Taken the advantage of gibberellin synthesis, Fusarium fujikuroi succeed in being a prevalent plant pathogen. At the meanwhile, F. fujikuroi was utilized for industrial production of gibberellins, a group of extensively applied phytohormone. F. fujikuroi has been known for its outstanding performance in gibberellin production for almost 100 years. Research activities relate to this species has lasted for a very long period. The slow development in biological investigation of F. fujikuroi is largely due to the lack of efficient research technologies and molecular tools. During the past decade, technologies to analyze the molecular basis of host-pathogen interactions and metabolic regulations have been developed rapidly, especially on the aspects of genetic manipulation. At the meanwhile, the industrial fermentation technologies kept sustained development. In this article, we reviewed the currently available research tools/methods for F. fujikuroi research, focusing on the topics about genetic engineering and gibberellin production.

show abstract

Effects of exogenous application of CPPU, NAA and GA 4+7 on parthenocarpy and fruit quality in cucumber ( Cucumis sativus L.)

Cited by 84 publications

References 18 publications

PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

Comprehensive Analysis of Cucumber Gibberellin Oxidase Family Genes and Functional Characterization of CsGA20ox1 in Root Development in Arabidopsis

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

Contact Info

Product

Resources

About