Effects of exogenous 6-BA and NAA on growth and contents of medicinal ingredient of Phellodendron chinense seedlings

Abstract

Using seedlings as material, and treated with different concentrations of exogenous 6-Benzylaminopurine (6-BA) and α-naphthyacetic acid (NAA), then observed the growth status. Furthermore, we detected the contents of chlorophyll and soluble sugar, the activities of antioxidases by spectrophotometry, and determined the contents of secondary metabolite by high performance liquid chromatograph. The results showed that different concentrations of exogenous 6-BA increases the fresh weights and plant heights of seedlings, and enhances the contents of chlorophyll and soluble sugar. NAA promoted growth, but deduced the contents of soluble sugar. Compared with control, culturing for 40 d, proper concentrations 6-BA enhanced the activity levels of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), proper concentrations NAA increased the activity levels of SOD and CAT, but decreased the levels of POD compared with CK. Suitable concentrations 6-BA enhanced contents of berberine, phellodendrine and palmatine in stems, proper concentrations NAA increased contents of berberine and phellodendrine, but deduced contents of palmatine compared with CK. Based on these results, we concluded that the exogenous 6-BA and NAA had key regulation on the growth and contents of medicinal ingredient of seedlings.

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

“…When leaves of O. Fragrance were under high temperature stress, ADH gene expression would be increased because of the resistance response mechanism activation in order to avoid from heat damage. Many researches about functional components in the leaves, flowers (Ge et al, 2018a and2018b), barks (Ge et al, 2017a and, stems (Ge et al, 2017c and2017d), roots (Ge et al, 2017e;, fruits (Jiang et al, 2018a and2018b), timbers (Peng et al, 2017a and and byproducts (Liu et al, 2017;Xie et al, 2018) of plants showed that they contain many bioactive components (Peng et al, 2016;He et al, 2017;Cheng et al, 2017;Yang et al, 2017;Qin et al, 2017;Xu et al, 2018;Wang et al, 2018). This result was consistent with the resistance response function of ADH (Strommer et al, 2011).…”

Foliar Spraying of 6-Benzylaminopurine Promotes Growth and Flavonoid Accumulation in Mulberry (Morus alba L.)

“…Plant hormones play critical roles in plant growth and differentiation as well as the accumulation of medicinal ingredients (Marı´n et al 2001;Liu et al 2018;He et al 2018). Plant hormones increase the expression of related enzyme genes in the secondary metabolite synthesis pathway, thereby increasing enzyme activity and ultimately increasing the accumulation of secondary metabolites.…”

Functional nano-catalyzed pyrolyzates from branch of Cinnamomum camphora

“…Tetradecanal (0.41%) is an edible spice that is used to mix peach, fat and milk flavors. The degradation products of Neophytadiene (1.34%) have an important contribution to tobacco aroma (He et al, 2018). 2-Propanone, 1-(4-hydroxy-3-methoxyphenyl) - (1.86%) can release volatile aromatic compounds, which contribute to the aroma of barrel wine (Truong et al, 2017).…”