Effects of excimer laser on healing of articular cartilage in rabbits

Athanasiou, K. A.; Renate, Fischer; Niederauer, G. G.; Puhl, W.

doi:10.1002/jor.1100130403

Cited by 32 publications

(21 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…61,62 Experiments in rabbits demonstrated the growth of neogenetic cartilage after nonablative laser treatment of the spinal disks in only the irradiated zones. 55,73,78,79 The histological and electron microscopic examinations verified formation of hyaline-like and fibrous-hyaline cartilages (Figs. 7 and 8) in the modeled defects of the NP in two and six months after laser treatment.…”

Section: Laser Reconstruction Of Spine Disksmentioning

confidence: 92%

“…It was concluded that many studies are not scientifically substantiated and future investigations should be based on sound biological foundations. Later, Athanasiou et al 73 studied the effect of an excimer laser on the healing of articular cartilage in a rabbit's knee. Chondral shaving and subchondral abrasion of cartilage, by creating partial-thickness and full-thickness cartilage defects of standardized size, were imitated with both an excimer laser and drilling.…”

Section: Experimental and Clinical Studies Prospects Of Laser Regenementioning

confidence: 99%

“…55,73,78,79 In the course of LRD, laser radiation of 1.5 W in power was delivered to the NP of the damaged disk through an optical fiber using a needle puncture. All zones of the NP were irradiated by a series of laser pulses (pulse duration was of 2 s, interval between pulses was of 1 s).…”

Section: Laser Reconstruction Of Spine Disksmentioning

confidence: 99%

See 2 more Smart Citations

Laser-induced regeneration of cartilage

et al. 2011

View full text Add to dashboard Cite

Abstract. Laser radiation provides a means to control the fields of temperature and thermo mechanical stress, mass transfer, and modification of fine structure of the cartilage matrix. The aim of this outlook paper is to review physical and biological aspects of laser-induced regeneration of cartilage and to discuss the possibilities and prospects of its clinical applications. The problems and the pathways of tissue regeneration, the types and features of cartilage will be introduced first. Then we will review various actual and prospective approaches for cartilage repair; consider possible mechanisms of laser-induced regeneration. Finally, we present the results in laser regeneration of joints and spine disks cartilages and discuss some future applications of lasers in regenerative medicine. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

show abstract

Section: Laser Reconstruction Of Spine Disksmentioning

confidence: 92%

Section: Experimental and Clinical Studies Prospects Of Laser Regenementioning

confidence: 99%

See 1 more Smart Citation

Laser-induced regeneration of cartilage

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Associated with the abnormal composition of such repair tissue is a deficiency in biomechanical function [2,15,46]. Because of the ineffectiveness of intrinsic repair, methods for stimulating cartilage repair are under active investigation…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the objectives were (1) to determine the effects of culture conditions (serum and duration of culture) on the formation of a CM by adult bovine chondrocytes; (2) to optimize the formation of a cartilaginous tissue by the chondrocytes after their recovery from the alginate gel; (3) and to determine if the proteoglycan (PG) and collagen components that accumulate into the matrix of the cartilage constructs are representative of those present in native articular cartilage.…”

Section: Introductionmentioning

confidence: 99%

A novel two‐step method for the formation of tissue‐engineered cartilage by mature bovine chondrocytes: The alginate‐recovered‐chondrocyte (ARC) method

Masuda¹,

Sah

Hejna

et al. 2003

Journal Orthopaedic Research

242

185

View full text Add to dashboard Cite

Most attempts to tissue-engineer cartilage have involved seeding of cultured cells into a biological or synthetic scaffold. We have developed a novel two-step culture approach that makes possible the in vitro formation of cartilaginous-like tissue by mature adult bovine chondrocytes without the aid of a synthetic matrix. The first step consists of culturing chondrocytes under conditions that maintain their rounded shape and their molecular phenotype as assessed by type I1 collagen and aggrecan production. This step was accomplished by culturing the isolated chondrocytes in alginate beads until the cells have reestablished a proteoglycan-rich cellassociated matrix (CM). The second step consists of culturing the cells with their CM, after recovery from the beads, on a tissue culture insert with a porous membrane. In this study, young adult bovine articular chondrocytes were cultured in alginate beads in the presence of 10% or 20%) fetal bovine serum (FBS). After 7 days of culture, the alginate beads were dissolved by incubating the beads for 20 min in sodium citrate buffer, a calcium chelator. Following a brief centrifugation, the cells with their CM were recovered, resuspended in medium containing 10% or 20% FBS and seeded onto a tissue culture insert. After 1 week of culture on the insert, the individual cells with their CM progressively became incorporated into a mass of cartilaginous tissue. Culture with 20% FBS resulted in the best formation of tissues. These tissues, easily recovered from the insert, were then subjected to biochemical and histological analyses. The biochemical results showed that the chondrocytes remain phenotypically stable in the tissues. The de novo tissue has a relatively high ratio of PGkollagen. Histological examination of the tissue revealed it contained a cartilage-like matrix strongly stained with toluidine blue. This scaffold-free system appears ideal to study, in vitro, the development of transplantable cartilaginous tissue.

show abstract

Integrin‐mediated adhesion of human articular chondrocytes to cartilage

Kurtis

Schmidt

Bugbee

et al. 2003

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To determine 1) the kinetics and strength of adhesion of human articular chondrocytes to a cut cartilage surface, and 2) the role of specific integrins in mediating such adhesion, using an in vitro model.Methods. Human articular chondrocytes isolated from cadaveric donors (mean ؎ SD age 38 ؎ 13 years) were cultured in high-density or low-density monolayer. Following release from culture with trypsin and a 2-2.5-hour recovery period, chondrocytes were analyzed either for adhesion to cartilage or for integrin expression by flow cytometry.Results. Following culture in monolayer, adhesion of chondrocytes to cartilage increased with time, from 6-16% at 10 minutes to a maximum of 59-82% at 80-320 minutes. After 80 minutes of adhesion, the resistance of cells to flow-induced shear stress (50% detachment) was ϳ21 Pa. Chondrocyte adhesion to cartilage decreased with pretreatment of cells with monoclonal antibodies that bound to and blocked certain integrins. After an 80-minute incubation time, adhesion of chondrocytes cultured in high-density monolayer decreased from the value of IgG1-treated controls (55%) with blocking of the ␤1 integrin subunit (to 23%) or with blocking of ␣5␤1 (to 36%). Following expansion of chondrocytes in low-density monolayer, the mechanisms of adhesion to cartilage were generally similar. After an 80-minute incubation time, adhesion of chondrocytes cultured in low-density monolayer decreased from the value of IgG1-treated controls (62%) with blocking of the ␤1 integrin subunit (to 30%) or with blocking of ␣5␤1 (to 44%). Additionally, adhesion of these cells decreased to 46% by blocking of ␣v␤5, with a similar trend in effect for chondrocytes cultured in high-density monolayer. Blocking of the ␣1 or ␣3 integrin subunits or ␣v␤3 had no detectable effect on adhesion, even though these receptors were detected by flow cytometry.Conclusion. Under the culture and seeding conditions studied, ␤1, ␣5␤1, and ␣v␤5 integrins mediate human chondrocyte adhesion to cartilage. These chondrocyte integrins have a potential role in the initial adhesion and retention of chondrocytes at a cartilage defect site following clinical procedures of chondrocyte transplantation.

show abstract

Effects of excimer laser on healing of articular cartilage in rabbits

Cited by 32 publications

References 26 publications

Laser-induced regeneration of cartilage

Laser-induced regeneration of cartilage

A novel two‐step method for the formation of tissue‐engineered cartilage by mature bovine chondrocytes: The alginate‐recovered‐chondrocyte (ARC) method

Integrin‐mediated adhesion of human articular chondrocytes to cartilage

Contact Info

Product

Resources

About