Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Jeong, Kiho; Lee, Dong Seok; Kim, Sang Ryong

doi:10.1016/j.eplepsyres.2015.06.001

Cited by 20 publications

(16 citation statements)

References 17 publications

(31 reference statements)

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“…A review study reported that some plant species possess essential oils compounds with anticonvulsant activity in experimental models of seizure [ 9 , 10 ]. They show many pharmacological effects on CNS, including anticonvulsant activity, and it is probably due to the high structural diversity of constituents, like the monoterpene epoxy-carvone [ 30 ], terpinen-4-ol [ 31 ], alpha and beta-pinene [ 32 ], and the phenylpropanoid eugenol [ 11 ]. TAN is a phenylpropanoid, obtained, principally, from the essential oil of star anise ( Illicium verum ), as a majority component [ 15 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the middle of plant species diversity, the “aromatic plants” have raised interest for their pharmacological and physiological actions, with several properties in the central nervous system (CNS), including antiepileptic activity [ 9 ]. These activities have generally been attributed to essential oils and/or their chemical constituents [ 10 ], such as phenylpropanoid [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Anticonvulsant Activity of trans-Anethole in Mice

Guedes

Ribeiro

Carneiro

et al. 2022

BioMed Research International

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Epilepsy is a chronic neurological disorder affecting 1-2% of world population, and one-third of patients are refractory to pharmacological treatment. This fact has stimulated research for new antiepileptic drugs and natural products have been an important source. trans-Anethole (TAN) is a phenylpropanoid, component of some essential oils, extracted from plants, and its effects have been little studied. Therefore, this study is aimed at investigating the TAN effect in classic seizure models and evaluate the electroencephalographic (EEG) profile of animals treated with this substance. For this, Swiss male mice (Mus musculus) were used, and the lethal dose was evaluated and subsequently submitted to the test maximal electroshock (MES), the pentylenetetrazole- (PTZ) induced seizure test, and the EEG profile. Initially, the LD50 for TAN was estimated in 1000 mg/kg (i.p.) dose and there was no sign of acute toxicity or death. In the MES test, TAN 300, i.p. ( 12.00 ± 2.9 s) and 400 mg/kg, i.p. ( 9.00 ± 4.4 s) doses was able to decrease tonic seizures duration induced by electric discharge (0.5 mA, 150 pulses/s, for 0.5 s). In the PTZ test (75 mg/kg, i.p.), TAN 400 mg/kg, i.p. increased the latency to myoclonic jerks (80.0 (56.0–134.0)), the latency totonic-clonic seizures (900.0 (861.0–900.0) and decrease seizure duration (0.0 (0.0–10.0)). No deaths were found in this groups compared to vehicle. EEG analysis showed an amplitude decrease of waves (ratio of baseline) in TAN 300 ( 1.82 ± 0.23 ) and 400 mg/kg ( 1.06 ± 0.16 ) groups. In this way, TAN at 400 mg/kg was able to inhibit and/or attenuate seizures by increasing the time for the onset of spasms and convulsions, as reducing the duration of seizures. The EEG profile corroborate with this results showing a reduction in the amplitude of waves compared to the PTZ group. Thus, TAN showed an anticonvulsant effect in all experimental models performed, behavioral and electroencephalographic.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticonvulsant Activity of trans-Anethole in Mice

Guedes

Ribeiro

Carneiro

et al. 2022

BioMed Research International

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“…Brain tissues were prepared for western blot analysis, as previously described (Jeong, Lee, & Kim, 2015). Mice hippocampal tissues were homogenized and centrifuged at 4°C for 15 min at 14,000 g .…”

Section: Methodsmentioning

confidence: 99%

Vascular endothelial growth factor receptor‐3 regulates astroglial glutamate transporter‐1 expression via mTOR activation in reactive astrocytes following pilocarpine‐induced status epilepticus

Jeong

Cho

Lee

et al. 2020

Glia

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Recent evidence has shown that the vascular endothelial growth factor (VEGF) system plays a crucial role in several neuropathological processes. We previously reported an upregulation of VEGF-C and its receptor, VEGFR-3, in reactive astrocytes after the onset of status epilepticus (SE). However, it remains unknown, which molecules act as downstream signals following VEGFR-3 upregulation, and are involved in reactive astrogliosis after SE. Therefore, we investigated whether VEGFR-3 upregulation within reactive astrocytes is associated with the activation of mammalian target of rapamycin (mTOR) signaling, which we confirmed by assaying for the phosphorylated form of S6 protein (pS6), and whether VEGFR-3-mediated mTOR activation induces astroglial glutamate transporter-1 (GLT-1) expression in the hippocampus after pilocarpine-induced SE. We found that spatiotemporal expression of pS6 was consistent with VEGFR-3 expression in the hippocampus after SE, and that both pS6 and VEGFR-3 were highly expressed in SE-induced reactive astrocytes. Treatment with the mTOR inhibitor rapamycin decreased astroglial VEGFR-3 expression and GLT-1 expression after SE. Treatment with a selective inhibitor for VEGFR-3 attenuated astroglial pS6 expression as well as suppressed GLT-1 expression and astroglial reactivity in the hippocampus after SE. These findings demonstrate that VEGFR-3-mediated mTOR activation could contribute to the regulation of GLT-1 expression in reactive astrocytes during the subacute phase of epilepsy. In conclusion, the present study suggests that VEGFR-3 upregulation in reactive astrocytes may play a role in preventing hyperexcitability induced by continued seizure activity.

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“…Jeong et al [205] evaluated the inhibitory effect of eugenol after unilateral injection of kainic acid in the hippocampus; there was significant delay in seizure onset. At 200 mg/kg i.p., eugenol decreased the appearance of granule cell dispersion (characteristic of temporal lobar epilepsy) by 52%.…”

Section: Eugenolmentioning

confidence: 99%

Anticonvulsant Essential Oils and Their Relationship with Oxidative Stress in Epilepsy

et al. 2019

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Epilepsy is a most disabling neurological disorder affecting all age groups. Among the various mechanisms that may result in epilepsy, neuronal hyperexcitability and oxidative injury produced by an excessive formation of free radicals may play a role in the development of this pathology. Therefore, new treatment approaches are needed to address resistant conditions that do not respond fully to current antiepileptic drugs. This paper reviews studies on the anticonvulsant activities of essential oils and their chemical constituents. Data from studies published from January 2011 to December 2018 was selected from the PubMed database for examination. The bioactivity of 19 essential oils and 16 constituents is described. Apiaceae and Lamiaceae were the most promising botanical families due to the largest number of reports about plant species from these families that produce anticonvulsant essential oils. Among the evaluated compounds, β-caryophyllene, borneol, eugenol and nerolidol were the constituents that presented antioxidant properties related to anticonvulsant action. These data show the potential of these natural products as health promoting agents and use against various types of seizure disorders. Their properties on oxidative stress may contribute to the control of this neurological condition. However, further studies on the toxicological profile and mechanism of action of essential oils are needed.

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Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Cited by 20 publications

References 17 publications

Anticonvulsant Activity of trans-Anethole in Mice

Anticonvulsant Activity of trans-Anethole in Mice

Vascular endothelial growth factor receptor‐3 regulates astroglial glutamate transporter‐1 expression via mTOR activation in reactive astrocytes following pilocarpine‐induced status epilepticus

Anticonvulsant Essential Oils and Their Relationship with Oxidative Stress in Epilepsy

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