Effects of Ethanol on Photoreceptors and Visual Function in Developing Zebrafish

Matsui, Jonathan I.; Egaña, Ana L.; Sponholtz, Todd R.; Adolph, Alan R.; Dowling, John E.

doi:10.1167/iovs.05-0971

Cited by 76 publications

(81 citation statements)

References 45 publications

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“…In zebrafish, ethanol exposure to embryos results in phenotypes similar to those seen in other animal models, and in humans (Blader & Strahle, 1998;Bilotta et al, 2002Bilotta et al, , 2004Carvan et al, 2004;Arenzana et al, 2006;Matsui et al, 2006). Zebrafish embryos are semi-transparent and develop externally over a very short, and experimentally manipulable, period.…”

Section: Introductionmentioning

confidence: 89%

“…Photoreceptor development is sensitive to ethanol exposure from 48 to 72 hpf (Matsui et al, 2006). To determine if ethanol exposure from 24 to 48 hpf affects subsequent photoreceptor differentiation, treated embryos were transferred to unmodified system water, and then processed at 72 hpf for indirect immunofluorescence using zpr1, an antibody that labels a cell-surface epitope on red/green double cones (Larison & Bremiller, 1990) and zpr3, an antibody that labels rod photoreceptors.…”

Section: Ethanol Exposure Affects Retinal Cell Differentiationmentioning

confidence: 99%

“…Potential mechanisms mediating the effects of ethanol exposure on the visual system can be tested through the use of molecular and genetic methods (Easter & Malicki, 2002). Studies pursuing effects of ethanol on the visual system have shown effects on eye morphogenesis and growth (Blader & Strahle, 1998;Bilotta et al, 2004), retinal lamination (Arenzana et al, 2006), photoreceptor differentiation and optic nerve development (Matsui et al, 2006), and visual function (Bilotta et al, 2002;Matsui et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

2007

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The exposure of the developing human embryo to ethanol results in a spectrum of disorders involving multiple organ systems, including the visual system. One common phenotype seen in humans exposed to ethanol in utero is microphthalmia. The objective of this study was to describe the effects of ethanol during retinal neurogenesis in a model organism, the zebrafish, and to pursue the potential mechanisms by which ethanol causes microphthalmia. Zebrafish embryos were exposed to 1% or 1.5% ethanol from 24 to 48 h after fertilization, a period during which the retinal neuroepithelium undergoes rapid proliferation and differentiation to form a laminated structure composed of different retinal cell types. Ethanol exposure resulted in significantly reduced eye size immediately following the treatment, and this microphthalmia persisted through larval development. This reduced eye size could not entirely be accounted for by the accompanying general delay in embryonic development. Retinal cell death was only slightly higher in ethanol-exposed embryos, although cell death in the lens was extensive in some of these embryos, and lenses were significantly reduced in size as compared to those of control embryos. The initiation of retinal neurogenesis was not affected, but the subsequent waves of cell differentiation were markedly reduced. Even cells that were likely generated after ethanol exposure-rod and cone photoreceptors and Müller glia-were delayed in their expression of cell-specific markers by at least 24 h. We conclude that ethanol exposure over the time of retinal neurogenesis resulted in persistent microphthalmia due to a combination of an overall developmental delay, lens abnormalities, and reduced retinal cell differentiation.

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Section: Introductionmentioning

confidence: 89%

Section: Ethanol Exposure Affects Retinal Cell Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

2007

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“…These changes in the eye movements indicated that acute ethanol treatment may impair the vision function in zebrafish larvae. 13,32 D. Analysis of cardiac function in response to ethanol treatment…”

Section: Ethanol-induced Behavioral Responses Of Zebrafishmentioning

confidence: 99%

“…10,11 For example, it was reported that acute ethanol treatment could adversely affect neural development. 12,13 Ethanol exposure has also been shown to influence the histaminergic and dopaminergic systems and to stimulate the locomotion in larval zebrafish. 2,14 However, little has been done to associatively study ethanol-induced acute responses in different organ systems, and to simultaneously address brain and cardiac physiology with direct links to behavioral alternations, mainly due to the lack of technology that can perform behavioral study and cellular imaging in an integrated format.…”

Section: Introductionmentioning

confidence: 99%

Autonomous system for cross-organ investigation of ethanol-induced acute response in behaving larval zebrafish

Lin

Chen

et al. 2016

Biomicrofluidics

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Ethanol is widely consumed and has been associated with various diseases in different organs. It is therefore important to study ethanol-induced responses in living organisms with the capability to address specific organs in an integrative manner. Here, we developed an autonomous system based on a series of microfluidic chips for cross-organ investigation of ethanol-induced acute response in behaving larval zebrafish. This system enabled high-throughput, gel-free, and anesthetic-free manipulation of larvae, and thus allowed real-time observation of behavioral responses, and associated physiological changes at cellular resolution within specific organs in response to acute ethanol stimuli, which would otherwise be impossible by using traditional methods for larva immobilization and orientation. Specifically, three types of chips ("motion," "lateral," and "dorsal"), based on a simple hydrodynamic design, were used to perform analysis in animal behavior, cardiac, and brain physiology, respectively. We found that ethanol affected larval zebrafish in a dose-dependent manner. The motor function of different body parts was significantly modulated by ethanol treatment, especially at a high dose of 3%. These behavioral changes were temporally associated with a slow-down of heart-beating and a stereotyped activation of certain brain regions. As we demonstrated in this proof-of-concept study, this versatile Fish-on-Chip platform could potentially be adopted for systematic cross-organ investigations involving chemical or genetic manipulations in zebrafish model. Published by AIP Publishing. [http://dx

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Locomotion and Behavioral Toxicity in Larval Zebrafish: Background, Methods, and Data

et al. 2011

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Effects of Ethanol on Photoreceptors and Visual Function in Developing Zebrafish

Abstract: Ethanol affects photoreceptor function at low concentrations that do not disturb retinal morphology. Higher levels of ethanol inhibit photoreceptor development and cause hypoplasia of the optic nerve.

Cited by 76 publications

References 45 publications

Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

Mechanisms for persistent microphthalmia following ethanol exposure during retinal neurogenesis in zebrafish embryos

Autonomous system for cross-organ investigation of ethanol-induced acute response in behaving larval zebrafish

Locomotion and Behavioral Toxicity in Larval Zebrafish: Background, Methods, and Data

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