Effects of Ethanol Extracts from Petasites japonicus S. et Z. Max. on Hepatic Antioxidative Systems in Alcohol Treated Rats

조배식,; 이재준,; 이명렬,

doi:10.3746/jkfn.2007.36.3.298

Cited by 9 publications

(8 citation statements)

References 14 publications

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“…Among the six, four peaks (peaks 1, 2, 3 and 5) showed relatively strong ABTS radical scavenging activity, while the other two peaks showed weak (peak 4) or even no (peak 6) scavenging activity. Therefore, the main antioxidant compounds in the leaf extract of P. japonicus were peaks 1, 2, 3 and 5, which accounted for the previously reported antioxidant activity of leaf extracts in the literature (Cho et al, 2007;Shang et al, 2010). The UV spectra of these four peaks obtained from DAD in HPLC demonstrated a similar distinctive pattern, where λ max values were 217-218 and 325 nm (Fig.…”

Section: Resultssupporting

confidence: 69%

“…Therefore, the main antioxidant compounds in the leaf extract of P . japonicus were peaks 1 , 2 , 3 and 5 , which accounted for the previously reported antioxidant activity of leaf extracts in the literature (Cho et al , ; Shang et al , ). The UV spectra of these four peaks obtained from DAD in HPLC demonstrated a similar distinctive pattern, where λ max values were 217–218 and 325 nm (Fig.…”

Section: Resultssupporting

confidence: 65%

“…is a commonly consumed vegetable in Korean and Japanese cultures. A number of studies have been carried out on the isolation of its biologically active constituents (Mizushina et al , ; Sakamura et al , ; Shimoda et al , ) and on the pharmaceutical functions of extracts or pure compounds from this plant (Cho et al , ; Seo et al , ; Watanabe et al , ). In the present study, we applied two hyphenated‐HPLC techniques, LC‐NMR and LC‐ion‐trap‐ESI‐MS, for the structural identification of the main compounds in methanol extracts of leaves and roots of P .…”

Section: Introductionmentioning

confidence: 99%

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Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated‐HPLC techniques

Kim¹,

Kang²,

Jeon

et al. 2011

Biomedical Chromatography

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Gradient HPLC coupled to Diode Array Detector (DAD), MS/MS and NMR was applied to the rapid structure determination of major compounds of methanol extracts from leaves and roots of Petasites japonicus. The relative antioxidant capacities of the compounds were evaluated by an HPLC system with post-column on-line antioxidant detection based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging. Six compounds were successfully separated on a reverse-phase C(18) column and were identified as 5-caffeoylquinic acid (5-CQA), fukinolic acid (FA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), quercetin-3-O-(6″-acetyl)-β-glucopyranoside (QAG), 4,5-di-O-caffeoylquinic acid (4,5-DCQA) and kaempferol-3-O-(6″-acetyl)-β-glucopyranoside (KAG) by MS/MS and (1)H NMR data. Among these compounds, those containing a caffeoyl moiety (5-CQA, FA, 3,5- and 4,5-DCQA) showed relatively strong radical scavenging capacity, with 3,5-DCQA having the greatest radical scavenging capacity in leaf (23.09% of total antioxidant capacity) and root (26.47%) extracts. The relative radical scavenging portion of QAG was only 3.41% in the leaves and KAG did not show any radical scavenging activity. These results demonstrate that the hyphenated HPLC techniques can be successfully applied to rapidly identify structures and evaluate antioxidant activities without prior purification of compounds from plant tissues of P. japonicus.

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Section: Resultssupporting

confidence: 69%

Section: Resultssupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated‐HPLC techniques

Kim¹,

Kang²,

Jeon

et al. 2011

Biomedical Chromatography

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“…특히 식품분야에서는 염증 반응성 을 감소시키거나 무력화할 수 있는 약물의 발굴과 활용 에 커다란 관심을 가지고 우리 주변에서 볼 수 있는 으 름열매 [13], 헛개나무 [14], 머위 [15], 모링가 [16], 오미자 은 활성이 유의적으로 감소하였다 (Fig. 1.…”

Section: 서론unclassified

Effects of Lespedeza Caneata ethanol extract on the liver of alcohol administered Mice

Cheong¹,

Chung²

2016

Journal of the Korea Academia-Industrial cooperation Society

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This study was conducted to investigate the effects of Lespedeza caneata extract on the livers of alcohol-administered mice. The study subjects were divided into a control (Con), alcohol administration (AL), alcohol and Lespedeza Caneata extract 200 ㎎/kg administration (AL-LC 200), and alcohol and Lespedeza caneata extract400 ㎎/kg administration (AL-LC 400) group. Distilled water was administrated orally to control and alcohol groups for ten days, while Lespedeza caneata extract was administered orally to alcohol and Lespedeza caneata extract groups for ten days. All experimental groups were fasted for twelve hours seven days after the oral administration, after which distilled water was administered orally to Con five times at twelve-hour intervals. At the same time, 50% ethanol (MERCK, USA) at 10 g/kg concentration was administered orally to AL and AL-LC groups five times at 12-hour intervals. The AST, ALT enzyme activation in blood and histology of the liver were then evaluated. AST and ALT in AL-LC groups were lower than in the AL group. Particularly, the AL-LC 200 and AL-LC 400 groups had significantly lower AST activation than the AL group. Histological results showed that most of the subjects in the AL group had necrosis and deformation in their livers, while fat droplets were accumulated in hepatic cells around the central vein. AL-LC 200 group revealed that a portion of the central vein was swollen, liver cells were expanded, and small fat droplets were accumulated. In the AL-CL 400 group, the central vein was normal and small fat droplets were accumulated in some liver cells. However, most of the liver cells appeared normal in the AL-CL 400 group. These results suggest that the extracts of Lespedeza caneata prevented alcohol induced liver damage in mice and have great potential for use as natural health products.

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“…Recently, the protective effect of an ethanol extract of P. japonicus S. et Z. Max. on hepatic antioxidative systems in alcohol treated rats has been published [14]. Some sesquiterpenes from the rhizomes of Curcuma zedoaria like the carabrane-type sesquiterpene lactones curcumenolactones A and B showed protective effects on D-galactosamine-induced cytotoxicity in primary cultured rat hepatocytes [15].…”

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confidence: 99%

Differential and StereoselectiveIn VitroCytotoxicity of Eremophilane Sesquiterpenes ofPetasites hybridusRhizomes in Rat Hepatocytes

Bodensieck¹,

Gaunitz²,

Gebhardt³

et al. 2010

Planta Med

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We tested two CO (2) extracts of Petasites hybridus L. rhizomes, A (rich in furanoeremophilanes) and B (rich in petasins), for IN VITRO cytotoxicity in rat hepatocytes by means of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (EC (50) values of 0.64 mg/mL for A and 0.32 mg/mL for B). Eight eremophilane sesquiterpene lactones (SL) (1- 8) and one petasin (9) isolated from A were nontoxic or showed moderate cytotoxicity. The presence and type of the ester side chain most probably control the extent of cytotoxicity of the eremophilanolides. (8 R)-2-[(angeloyl)oxy]eremophil-7(11)-en-12,8-olide (1) damaged the hepatocytes most. The 8 α-stereoisomers of both 8-H epimeric couples of the 2-angeloyloxy- and 2-methacroyloxy-esters seem to be more cytotoxic (up to approx. 10-fold) than the corresponding 8 β-H stereoisomers. Moreover, the results of the MTT assay depended on the cell density being more pronounced with both 8 α-stereoisomers. Further investigations were conducted to study the influence of the stereochemistry on cell respiration, energy metabolism, and membrane integrity [release of lactate dehydrogenase (LDH)] with both couples of the 2-angeloyloxy- and 2-methacroyloxy-esters. In the LDH-leakage assay, (8 R)-2-[(methacroyl)oxy]eremophil-7(11)-en-12,8-olide (2) was the most toxic eremophilane. The stereoselectivity of cell damage of some SL points to a specific, yet unidentified molecular cytotoxicity target.

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Effects of Ethanol Extracts from Petasites japonicus S. et Z. Max. on Hepatic Antioxidative Systems in Alcohol Treated Rats

Cited by 9 publications

References 14 publications

Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated‐HPLC techniques

Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated‐HPLC techniques

Effects of Lespedeza Caneata ethanol extract on the liver of alcohol administered Mice

Differential and StereoselectiveIn VitroCytotoxicity of Eremophilane Sesquiterpenes ofPetasites hybridusRhizomes in Rat Hepatocytes

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