Effects of Ethanol Exposure on Subsequent Acquisition and Extinction of Ethanol Self-Administration and Expression of Alcohol-Seeking Behavior in Adult Alcohol-Preferring (P) Rats: I. Periadolescent Exposure

Rodd-Henricks, Zachary A.; Bell, Richard L.; Kuc, Kelly A.; Murphy, James M.; McBride, William J.; Lumeng, Lawrence; Li, Ting‐Kai

doi:10.1097/00000374-200211000-00005

Cited by 48 publications

(93 citation statements)

References 41 publications

(22 reference statements)

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“…46,47 The P rat learns spontaneously to press the bar for oral alcohol in daily 60-minute sessions during the first 2–3 weeks of exposure. This phase of escalating response is termed “acquisition.” Eventually the animals develop a baseline response for alcohol over water, which is termed “maintenance.” 45,46 In the present study, the rats were not acclimated to the operant chamber before commencement of acquisition and did not receive any prior operant training. Both the EtOH (15%) and water levers were maintained on a fixed-ratio (FR) 1 schedule of reinforcement for the first 3 weeks, which means that the rats received 1 ethanol reward for each bar press.…”

Section: Methodsmentioning

confidence: 99%

Reduced ethanol consumption by alcohol-preferring (P) rats following pharmacological silencing and deep brain stimulation of the nucleus accumbens shell

Wilden

Qing

Hauser

et al. 2014

JNS

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Object There is increasing interest in deep brain stimulation (DBS) for the treatment of addiction. Initial testing must be conducted in animals, and the alcohol-preferring (P) rat meets the criteria for an animal model of alcoholism. This study is composed of 2 experiments designed to examine the effects of 1) pharmacological inactivation and 2) DBS of the nucleus accumbens shell (AcbSh) on the consumption of alcohol by P rats. Methods In the first experiment, the effects of reversible inactivation of the AcbSh were investigated by administering intracranial injections of γ–aminobutyric acid (GABA) agonists. Bilateral microinjections of drug were administered to the AcbSh in P rats (8–10 rats/group), after which the animals were placed in operant chambers containing 2 levers—one used to administer water and the other to administer 15% EtOH—to examine the acquisition and maintenance of oral EtOH self-administration. In the second experiment, a DBS electrode was placed in each P rat’s left AcbSh. The animals then received 100 or 200 μA (3–4 rats/group) of DBS to examine the effect on daily consumption of oral EtOH in a free-access paradigm. Results In the first experiment, pharmacological silencing of the AcbSh with GABA agonists did not decrease the acquisition of EtOH drinking behavior but did reduce EtOH consumption by 55% in chronically drinking rats. Similarly, in the second experiment, 200 μA of DBS consistently reduced EtOH intake by 47% in chronically drinking rats. The amount of EtOH consumption returned to baseline levels following termination of therapy in both experiments. Conclusions Pharmacological silencing and DBS of the AcbSh reduced EtOH intake after chronic EtOH use had been established in rodents. The AcbSh is a neuroanatomical substrate for the reinforcing effects of alcohol and may be a target for surgical intervention in cases of alcoholism.

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Section: Methodsmentioning

confidence: 99%

Reduced ethanol consumption by alcohol-preferring (P) rats following pharmacological silencing and deep brain stimulation of the nucleus accumbens shell

Wilden

Qing

Hauser

et al. 2014

JNS

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“…Previous research has shown that 2 weeks of home caging produced robust expression of EtOH seeking (Rodd-Henricks et al 2002a, b; Rodd et al 2006). Stereotaxic implantation was performed after 7 days in the home cage.…”

Section: Methodsmentioning

confidence: 96%

“…P rats were placed in the standard two-lever operant-conditioning chamber, as previously described (Rodd-Henricks et al 2002a, b; Rodd et al 2006; Hauser et al 2011, 2012a). Operant-conditioning sessions were 60 min in duration and occurred daily for 10 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…The FR5-FR1 schedule lever contingencies and dipper functioning were maintained, but EtOH and water were absent for the four consecutive PSR sessions (Hauser et al 2012a; Rodd et al 2006). There are four consecutive PSR sessions because previous studies have shown that exposure to EtOH odor cues or EtOH priming (Rodd-Henricks et al 2002a, b) and some drugs (Dhaher et al 2010) may enhance PSR responding for more than one session.…”

Section: Methodsmentioning

confidence: 99%

“…In this and all subsequent experiments, the micro-injections were given prior to only the first PSR session, starting 25–30 min before the session. The use of the electrolytic microinfusion transducer system has been shown to give good neuroanatomical specificity (Ikemoto et al 2006; Rodd-Henricks et al 2000, 2002a; Rodd et al 2007). …”

Section: Methodsmentioning

confidence: 99%

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Enhanced alcohol-seeking behavior by nicotine in the posterior ventral tegmental area of female alcohol-preferring (P) rats: modulation by serotonin-3 and nicotinic cholinergic receptors

et al. 2014

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Rationale Alcohol and nicotine co-use can reciprocally promote self-administration and drug-craving/drug-seeking behaviors. To date, the neurocircuitry in which nicotine influences ethanol (EtOH) seeking has not been elucidated. Clinical and preclinical research has suggested that the activation of the mesolimbic dopamine system is involved in the promotion of drug seeking. Alcohol, nicotine, and serotonin-3 (5-HT3) receptors interact within the posterior ventral tegmental area (pVTA) to regulate drug reward. Recently, our laboratory has reported that systemic administration of nicotine can promote context-induced EtOH seeking. Objectives The goals of the current study were to (1) determine if microinjections of pharmacologically relevant levels of nicotine into the pVTA would enhance EtOH seeking, (2) determine if coadministration of nicotinic cholinergic receptor antagonist (nACh) or 5-HT3 receptor antagonists would block the ability of nicotine microinjected into the pVTA to promote EtOH seeking, and (3) determine if 5-HT3 receptors in the pVTA can modulate EtOH seeking. Results Nicotine (100 and 200 µM) microinjected into the pVTA enhanced EtOH seeking. Coinfusion with 200 µM mecamylamine (nACh antagonist) or 100 and 200 µM zacopride (5-HT3 receptor antagonist) blocked the observed nicotine enhancement of EtOH seeking. The data also indicated that microinjection of 1 µM CPBG (5-HT3 receptor agonist) promotes context-induced EtOH seeking; conversely microinjection of 100 and 200 µM zacopride alone reduced context-induced EtOH seeking. Conclusions Overall, the results show that nicotine-enhanced EtOH-seeking behavior is modulated by 5-HT3 and nACh receptors within the pVTA and that the 5-HT3 receptor system within pVTA may be a potential pharmacological target to inhibit EtOH-seeking behaviors.

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Ethanol pre‐exposure during adolescence or adulthood increases ethanol intake but ethanol‐induced conditioned place preference is enhanced only when pre‐exposure occurs in adolescence

Carrara-Nascimento

Camarini

2012

Developmental Psychobiology

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Behavioral sensitization has been suggested to contribute to uncontrolled alcohol consumption. The aim of this study was to investigate the effects of repeated ethanol administration in adolescent and adult mice on subsequent ethanol consumption and conditioned place preference (CPP). Mice were administered ethanol for 15 consecutive days. This ethanol regimen induced behavioral sensitization to a lesser degree in adolescents than in adults. Following ethanol treatment, mice were subjected to CPP procedure, or given a free choice between water and ethanol solutions. While ethanol-pretreated adult mice did not display a robust ethanol-induced CPP, ethanol induced a significant CPP in mice pretreated with ethanol during adolescence. Ethanol pretreated mice, regardless of age, showed higher ethanol intake to saline-treated mice. The present findings suggest that ethanol-induced neuroadaptations underlying behavioral sensitization may activate mechanisms responsible for enhanced ethanol intake, and also reveals that ethanol pre-exposure during adolescence increases ethanol reward as measured by CPP.

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Effects of Ethanol Exposure on Subsequent Acquisition and Extinction of Ethanol Self-Administration and Expression of Alcohol-Seeking Behavior in Adult Alcohol-Preferring (P) Rats: I. Periadolescent Exposure

Cited by 48 publications

References 41 publications

Reduced ethanol consumption by alcohol-preferring (P) rats following pharmacological silencing and deep brain stimulation of the nucleus accumbens shell

Reduced ethanol consumption by alcohol-preferring (P) rats following pharmacological silencing and deep brain stimulation of the nucleus accumbens shell

Enhanced alcohol-seeking behavior by nicotine in the posterior ventral tegmental area of female alcohol-preferring (P) rats: modulation by serotonin-3 and nicotinic cholinergic receptors

Ethanol pre‐exposure during adolescence or adulthood increases ethanol intake but ethanol‐induced conditioned place preference is enhanced only when pre‐exposure occurs in adolescence

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