Effects of Ethanol Exposure on Nervous System Development in Zebrafish

Cole, Gregory J.; Zhang, Chengjin; Ojiaku, Princess; Bell, Vanessa; Devkota, Shailendra; Mukhopadhyay, Somnath

doi:10.1016/b978-0-12-394310-1.00007-2

Cited by 31 publications

(25 citation statements)

References 279 publications

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“…Animal models of FAS/FASD have been useful in probing the mechanisms for ethanol-induced congenital defects and in investigating potential preventative therapeutics such as folic acid, choline, and myoinositol (Smith, 1997; Sant'Anna and Tosello, 2006; Serrano et al, 2010; Wilson and Cudd, 2011; Ballard et al, 2012; Cole et al, 2012). …”

Section: Application Of Biophotonic Tools To Study the Genesis Of Chdmentioning

confidence: 99%

“…The etiology of FAS and FASD has been the focus of much study, especially the cellular and molecular mechanisms (e.g., reviewed in Cole et al, 2012; Ungerer et al, 2013; Veazey et al, 2013). Ethanol exposure at gastrulation has been shown to alter the expression of critical proteins (e.g., transcription factors) within the avian cardiac mesoderm at stage 9, before the heart begins to function (Serrano et al, 2010).…”

Section: Application Of Biophotonic Tools To Study the Genesis Of Chdmentioning

confidence: 99%

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Capturing structure and function in an embryonic heart with biophotonic tools

Karunamuni

Ford

et al. 2014

Front. Physiol.

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Disturbed cardiac function at an early stage of development has been shown to correlate with cellular/molecular, structural as well as functional cardiac anomalies at later stages culminating in the congenital heart defects (CHDs) that present at birth. While our knowledge of cellular and molecular steps in cardiac development is growing rapidly, our understanding of the role of cardiovascular function in the embryo is still in an early phase. One reason for the scanty information in this area is that the tools to study early cardiac function are limited. Recently developed and adapted biophotonic tools may overcome some of the challenges of studying the tiny fragile beating heart. In this chapter, we describe and discuss our experience in developing and implementing biophotonic tools to study the role of function in heart development with emphasis on optical coherence tomography (OCT). OCT can be used for detailed structural and functional studies of the tubular and looping embryo heart under physiological conditions. The same heart can be rapidly and quantitatively phenotyped at early and again at later stages using OCT. When combined with other tools such as optical mapping (OM) and optical pacing (OP), OCT has the potential to reveal in spatial and temporal detail the biophysical changes that can impact mechanotransduction pathways. This information may provide better explanations for the etiology of the CHDs when interwoven with our understanding of morphogenesis and the molecular pathways that have been described to be involved. Future directions for advances in the creation and use of biophotonic tools are discussed.

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Section: Application Of Biophotonic Tools To Study the Genesis Of Chdmentioning

confidence: 99%

Section: Application Of Biophotonic Tools To Study the Genesis Of Chdmentioning

confidence: 99%

Capturing structure and function in an embryonic heart with biophotonic tools

Karunamuni

Ford

et al. 2014

Front. Physiol.

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“…Prenatal exposure to ethanol results in a constellation of central nervous system (CNS) abnormalities that includes behavioral impairments, which are observed in a wide range of species (Gerlai et al, 2000; Cole et al, 2012; Ungerer et al, 2013). Studies in rodent have demonstrated that behavioral deficits similar to those observed in humans are produced following prenatal ethanol exposure.…”

Section: Introductionmentioning

confidence: 99%

Long-term behavioral change as a result of acute ethanol exposure in zebrafish: Evidence for a role for sonic hedgehog but not retinoic acid signaling

Burton¹,

Zhang²,

Boa-Amponsem

et al. 2017

Neurotoxicology and Teratology

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BACKGROUND Developmental exposure to ethanol is recognized to produce long-term neurobehavioral impairment in multiple animal models. However, the molecular mechanisms underlying these deficits remain poorly understood. The present study was undertaken to ascertain whether two well-characterized targets of prenatal alcohol exposure, sonic hedgehog (Shh) and retinoic acid (RA), that induce the hallmark morphological phenotypes of fetal alcohol spectrum disorders (FASD), are involved in the generation of behavioral alterations as a result of alcohol exposure. METHODS Zebrafish embryos were exposed to ethanol (0%, 1%, 3%) at either 8–10 or 24–27 hours post-fertilization (hpf) and then evaluated during adolescence in the novel tank dive test to assess anxiety and risk-taking behavior. Overt signs of dysmorphogenesis were also scored and behavioral and morphological changes were compared for embryos treated with alcohol alone or in combination with subthreshold doses of shh or alhh1a3 morpholinos (MOs). RESULTS Ethanol treated fish displayed altered tank diving behavior that was not exacerbated by combined MO treatment. While treatment of embryos with either shha mRNA or RA prior to ethanol exposure only ameliorated the altered tank diving response in the case of shha mRNA overexpression, dysmorphogenesis was rescued by both treatments. CONCLUSION These results suggest that the effects of ethanol exposure on changes in anxiety and risk-taking behavior in adolescent zebrafish is manifested by a blunting of Shh, but not RA, signaling during early development.

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“…The widespread effects of ethanol exposure on the fetal central nervous system (CNS) are well known to culminate in a constellation of behavioral impairments, a syndrome seen in a wide range of species (see reviews Cole et al, 2012; Gerlai et al, 2000; Ungerer et al, 2013). Moreover, it appears that the timing of ethanol exposure can be an important determinant of the CNS structures that are primarily affected and therefore presumably determines the nature of functional impairment (Hamre & West, 1993).…”

Section: Introductionmentioning

confidence: 99%

Long-term behavioral impairment following acute embryonic ethanol exposure in zebrafish

Bailey

Oliveri

Zhang

et al. 2015

Neurotoxicology and Teratology

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BACKGROUND Developmental exposure to ethanol has long been known to cause persisting neurobehavioral impairment. However, the neural and behavioral mechanisms underlying these deficits and the importance of exposure timing are not well-characterized. Given the importance of timing and sequence in neurodevelopment it would be expected that alcohol intoxication at different developmental periods would result in distinct neurobehavioral consequences. METHODS Zebrafish embryos were exposed to ethanol (0%, 1%, 3%) at either 8-10 or 24-27 hours post-fertilization (hpf) then reared to adolescence and evaluated on several behavioral endpoints. Habituation to a repeated environmental stimulus and overall sensorimotor function were assessed using a tap startle test; measurements of anxiety and exploration behavior were made following introduction to a novel tank; and spatial discrimination learning was assessed using aversive control in a three-chambered apparatus. Overt signs of dysmorphogenesis were also scored (i.e. craniofacial malformations, including eye diameter and midbrain-hindbrain boundary morphology). RESULTS Ethanol treated fish were more active both at baseline and following a tap stimulus compared to the control fish and were hyperactive when placed in a novel tank. These effects were more prominent following exposure at 24-27 hpf than with the earlier exposure window, for both dose groups. Increases in physical malformation were only present in the 3% ethanol group; all malformed fish were excluded from behavioral testing. DISCUSSION These results suggest specific domains of behavior are affected following ethanol exposure, with some but not all of the tests revealing significant impairment. The behavioral phenotypes following distinct exposure windows described here can be used to help link cellular and molecular mechanisms of developmental ethanol exposure to functional neurobehavioral effects.

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Effects of Ethanol Exposure on Nervous System Development in Zebrafish

Cited by 31 publications

References 279 publications

Capturing structure and function in an embryonic heart with biophotonic tools

Capturing structure and function in an embryonic heart with biophotonic tools

Long-term behavioral change as a result of acute ethanol exposure in zebrafish: Evidence for a role for sonic hedgehog but not retinoic acid signaling

Long-term behavioral impairment following acute embryonic ethanol exposure in zebrafish

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