Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae

Leão, Cecı́lia; Uden, N. van

doi:10.1016/0005-2736(84)90272-4

Cited by 182 publications

(129 citation statements)

References 14 publications

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“…The membrane potential and the proton gradient may also be affected by alcohols by inhibition of active proton transport or by increasing passive proton influx. 19) Furthermore, there are many experimental data proving that alcohol inhibition is correlated with the length of the non-polar carbon chain in their molecule, which also indicates that the inhibitory action takes place at the hydrophobic membrane sites, interfering directly with the transport proteins or indirectly by changing the lipid environment in the membrane. 20 ) In our experiments too, ethanol was more toxic than methanol, with the only exception of K j in case of C. utilis (Table).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Antoce¹,

Antoce²,

Takahashi³

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Antoce¹,

Antoce²,

Takahashi³

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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“…Such synergistic effects have been demonstrated for various combinations of acids, phenols and furans (Nigam 2001;Palmqvist et al 1999). Ethanol has also been reported to augment the toxicity of weak acids (Leã o and Van Uden 1984;Pampulha and Loureiro-Dias 1989).…”

Section: Common Inhibitors In Hydrolysates Effects and Mechanismsmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

432

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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“…These studies showed that hydrocarbons, e.g., 3-pinene (32) and cyclohexane (33), impaired energy transduction in both mitochondrial and plasma membranes of yeast cells. Studies on the toxic effects of ethanol on yeast cells indicated that the Ap across the plasma membrane was dissipated in the presence of ethanol (3), probably due to an increased influx of protons (3,12).In this investigation, the toxic action of the lipophilic compound tetralin on bacteria was studied. Tetralin was found to be toxic to bacterial cells at concentrations below 100 ,umol/liter (30).…”

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confidence: 97%

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confidence: 99%

Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes

et al. 1992

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Tetralin is toxic to bacterial cells at concentrations below 100 ,mollliter. To assess the inhibitory action of tetralin on bacterial membranes, a membrane model system, consisting of proteoliposomes in which beef heart cytochrome c oxidase was reconstituted as the proton motive force-generating mechanism, and several gram-positive and gram-negative bacteria were studied. Because of its hydrophobicity, tetralin partitioned into lipid membranes preferentially (lipid/buffer partition coefficient of tetralin is approximately 1,100). The excessive accumulation of tetralin caused expansion of the membrane and impairment of different membrane functions. Studies with proteoliposomes and intact cells indicated that tetralin makes the membrane permeable for ions (protons) and inhibits the respiratory enzymes, which leads to a partial dissipation of the pH gradient and electrical potential. The effect of tetralin on the components of the proton motive force as well as disruption of protein-lipid interaction(s) could lead to impairment of various metabolic functions and to low growth rates. The data offer an explanation for the difficulty in isolating and cultivating microorganisms in media containing tetralin or other lipophilic compounds.Interest in the application of water-immiscible organic compounds in fermentations has increased in the last decade. Many lipophilic compounds are harmful to microorganisms, impair growth, and even inhibit other biological reactions. Knowledge of the toxic action of lipophilic compounds on bacterial cells is mainly restricted to the relation between the hydrophobicity (13) of a compound and its effect on a specific enzyme (19). In most studies, the cytoplasmic membrane is mentioned as a possible target, but information about the nature of the toxic action is not presented (1,19).A correlation between the hydrophobicity of a compound and its effects on cells was first observed for anesthetics (27)(28)(29), which provided a basis for calculating a dose-effect relationship. In addition, the uncoupling effects of lipophilic compounds on energy transduction have been studied in animal cells (23). For microorganisms, only a few studies on the toxic effects of lipophilic compounds on various membrane functions have been performed (3,32,33). These studies showed that hydrocarbons, e.g., 3-pinene (32) and cyclohexane (33), impaired energy transduction in both mitochondrial and plasma membranes of yeast cells. Studies on the toxic effects of ethanol on yeast cells indicated that the Ap across the plasma membrane was dissipated in the presence of ethanol (3), probably due to an increased influx of protons (3,12).In this investigation, the toxic action of the lipophilic compound tetralin on bacteria was studied. Tetralin was found to be toxic to bacterial cells at concentrations below 100 ,umol/liter (30). Tetralin, 1,2,3,4-tetrahydronaphthalene, is a bicyclic molecule that consists of an aromatic and an alicyclic moiety. The compound is widely applied as an industrial solvent and as a substitute f...

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Effects of ethanol and other alkanols on passive proton influx in the yeast Saccharomyces cerevisiae

Cited by 182 publications

References 14 publications

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Quantitative Study of Yeast Growth in the Presence of Added Ethanol and Methanol Using a Calorimetric Approach

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Effects of the membrane action of tetralin on the functional and structural properties of artificial and bacterial membranes

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