Effects of ethane-1-hydroxy-1, 1-diphosphonate on cell differentiation, and proteoglycan and calcium metabolism, in the proximal tibia of young rats

Katoh, Yoshimasa; Tsuji, Haruo; Matsui, Hisao; Maruta, Kimiko; Morita, Yoshikazu

doi:10.1016/8756-3282(91)90001-y

Cited by 17 publications

(3 citation statements)

References 31 publications

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“…Bisphosphonates inhibit bone mineralization in vivo (10,11,(56)(57)(58)(59)(60)(61)(62), probably by a combination of the following three mechanisms: 1) a physicochemical mechanism lead ing to inhibition of crystal growth; 2) a direct inhibitory effect on bone formation in osteoblasts; and 3) as a result of the homeostatic coupling in bone, an inhibition of resorption is followed by an inhibition of formation; i.e., bone turnover is slowed down. The present data did not result from mechanism 3 at least since our human osteo blastic cell culture system contains no osteoclasts.…”

Section: Discussionmentioning

confidence: 99%

Alendronate Modulates Osteogenesis of Human Osteoblastic Cells In Vitro

Tsuchimoto

Azuma

Higuchi

et al. 1994

Japanese Journal of Pharmacology

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ABSTRACT-The bisphosphonates, which are carbon-substituted pyrophosphates, have been studied exten sively both in vivo and in vitro to elucidate their effects on bone tissues and cells. However, because these agents were shown to have a potent inhibitory effect on bone resorption, the majority of studies have focused on only this aspect of bone metabolism. There appears to be less information regarding the direct effect of bisphosphonates on bone formation, so thus we undertook experiments to investigate the effects of bisphosphonates, especially alendronate, on the mineralization and matrix protein synthesis of human osteoblastic cells in vitro. The data show that the bisphosphonates, alendronate, etidronate and pamidronate, suppressed 1,25-dihydroxycholecalciferol (1,25(OH)2D3)-stimulated mineralization of human osteoblastic cells at high concentrations, while relatively lower concentrations of alendronate and etidronate potentiated mineralization of the cells in the presence of 1,25(OH)2D3. The potentiation of mineralization with alendronate was accompanied by increased synthesis of bone matrix proteins, osteocalcin and col lagen, and the mRNA of pro a(I) collagen. These findings show that in addition to their well-known effects on bone resorption, bisphosphonates have significant and direct effects on osteogenesis in osteoblasts in vitro. The actual mechanism remains to be further investigated.

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Section: Discussionmentioning

confidence: 99%

Alendronate Modulates Osteogenesis of Human Osteoblastic Cells In Vitro

Tsuchimoto

Azuma

Higuchi

et al. 1994

Japanese Journal of Pharmacology

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“…This is recognised by investigators' descriptive comments on the sizes of osteoCorrespondence to: S.J. Jones clasts seen on the bone surface in various pathological (Kukita et al 1990) or experimental conditions (Katoh et al 1991), although a negative relationship may also be present (Shapiro et al 1988).…”

Section: Introductionmentioning

confidence: 99%

The relationship between the number of nuclei of an osteoclast and its resorptive capability in vitro

1992

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This study examined the relationship between the number of nuclei in an osteoclast and its resorptive efficiency, as demonstrated by the size of the pit it can make in a mineralized tissue in 24 h in vitro. Osteoclasts released mechanically from prehatch chick long bones were cultured on dentine slices or on plastic dishes for periods of 6 or 24 h. The frequency distribution of the multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells with different numbers of nuclei was determined: the mean number of nuclei per cell was 6.92, with a mode of 4.47% had 5 or fewer nuclei and only 11% more than 10 nuclei. The pits associated with 292 osteoclasts with known numbers of nuclei were measured using a confocal laser light microscope (Lasertec) and dedicated image analysis system, and depths, plan areas and volumes determined. There was a positive correlation between the number of nuclei per osteoclast and the volume of the pit made, but a trend for the volume resorbed per nucleus to decrease with increase in the number of nuclei per osteoclast.

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“…A (KATOH et al, 1991;SCHENK et al, 1973 et al, 2004;KAJI et al, 2006;LIU E LI, 2010;YASUI et al, 2011 -CHAVEZ, 2007;YAMAMOTO-SILVA et al, 2013). Outros estudos têm demonstrado que o alendronato aumenta o número de osteoclastos (FISHER et al, 2013) e que a origem destas células, bem como a região de reabsorção, podem interferir neste fenômeno (DE SOUZA FALONI et al, 2011).…”

Section: Genes Ligados à Osteogêneseunclassified

Estudo do efeito de bisfosfonatos nas células clásticas durante a ossificação endocondral do joelho de ratos e em cultura primária: abordagens morfológicas e moleculares.

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Effects of ethane-1-hydroxy-1, 1-diphosphonate on cell differentiation, and proteoglycan and calcium metabolism, in the proximal tibia of young rats

Cited by 17 publications

References 31 publications

Alendronate Modulates Osteogenesis of Human Osteoblastic Cells In Vitro

Alendronate Modulates Osteogenesis of Human Osteoblastic Cells In Vitro

The relationship between the number of nuclei of an osteoclast and its resorptive capability in vitro

Estudo do efeito de bisfosfonatos nas células clásticas durante a ossificação endocondral do joelho de ratos e em cultura primária: abordagens morfológicas e moleculares.

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