Effects of Estrogens on Platelets and Megakaryocytes

Dupuis, Marion; Séverin, Sonia; Noirrit‐Esclassan, Emmanuelle; Arnal, Jean‐François; Payrastre, Bernard; Valera, Marie-Cécile

doi:10.3390/ijms20123111

Cited by 36 publications

(26 citation statements)

References 64 publications

(81 reference statements)

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“…This induction of hypercoagulation indicates an interplay between oestrogen-induced platelet activation as well as activation due to the cytotoxic nature of Tamoxifen itself 15 – 17 . The precise mechanism by which Tamoxifen exerts its procoagulant effect remains to be discovered 7 , 18 ; however, studies implicate the anti-oestrogenic activity of the drug on oestrogen receptor alpha (ERα), particularly on those expressed by platelets , to further increase the risk of VTE 19 , 20 ; and further effects on Factor VIII in promoting thrombotic events via its role in platelet activation 18 .…”

Section: Introductionmentioning

confidence: 99%

Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo

Pather

Augustine

2020

Sci Rep

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Tamoxifen shows efficacy in reducing breast cancer-related mortality but clinically, is associated with increased risk for thromboembolic events. We aimed to determine whether breast tumour sub-phenotype could predict propensity for thrombosis. We present two ex vivo Models of Tamoxifen-therapy, Model 1 in which treatment recapitulates accumulation within breast tissue, by treating MCF7 and T47D cells directly prior to exposure to blood constituents; and Model 2 in which we recreate circulating Tamoxifen by treating blood constituents prior to exposure to cancer cells. Blood constituents included whole blood, platelet-rich plasma and platelet-poor plasma. Hypercoagulation was assessed as a function of thrombin activity, expression of CD62P and CD63 activation markers defined as an index of platelet activation, and platelet morphology; while oestrogen receptor expression was assessed using immunocytochemistry with quantitative analysis. We determined, in concert with clinical studies and contrary to selected laboratory investigations, that Tamoxifen induces hypercoagulation, dependent on sub-phenotypes, with the T47D cell line capacity most enhanced. We determined a weak positive correlation between oestrogen receptor expression, and CD62P and CD63; indicating an association between tumour invasion profiles and hypercoagulation, however, other yet unknown factors may play a predictive role in defining hypercoagulation.

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Section: Introductionmentioning

confidence: 99%

Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo

Pather

Augustine

2020

Sci Rep

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“…Many trials done previously have found that platelet function reduces significantly following oestrogen therapy. 25 The proposed reason was that oestrogen increases the prostaglandin level which in turn reduces platelet aggregation and function. However, the exact mechanism of how oestrogen increases prostaglandin level remains unknown.…”

Section: Resultsmentioning

confidence: 99%

Study of association of bleeding and clotting time with blood group among young adults

Benjamin¹,

Geetha²

2021

IJCAP

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Background: Blood group plays a unique role in revealing the identity of an individual. Several studies have expressed variations in bleeding tendencies among individuals with various ABO blood groups. Hence a study was done to the find association of bleeding and clotting time with this blood group. Materials and Methods: This study had a cross-sectional study design and was done among 250 young adults. Slide agglutination method was used to assess ABO blood group; while Dukes and capillary tube method were used to determine bleeding and clotting time respectively. Chi-square analysis was done to analyze its association with blood group. Results: Blood group O (38.4%) was found to be the major blood group among both genders, followed by B (34%), A (19.2%), AB (0.08%). Bleeding time of more than 4 minutes was found in both O and B group but the result was statistically not significant (p=0.85). Clotting time of more than 6 minutes was found again in both O and B groups and the difference was not significant (p=0.96). Bleeding time was found to be higher in males while Clotting time was found to be more prolonged in females however the difference was statistically less significant (p>0.05). Conclusion: In this study, results have shown that blood group O was the predominant blood group among the study population and blood group O and B had prolonged bleeding and clotting time compared to other groups. Based on gender, females had higher clotting time compared to males.

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“…The gender influence on platelet biology was shown several years ago [26] and recent studies reported a higher number of fibrinogen surface receptors and a greater ability to bind fibrinogen in women [27]. In addition, estrogens have been shown to enhance platelet production [28]. All these data are important since a direct association has been shown between platelet count and major cardiovascular risk factors [29].…”

Section: Discussionmentioning

confidence: 97%

Hydroxyvitamin D Serum Levels are Negatively Associated with Platelet Number in a Cohort of Subjects Affected by Overweight and Obesity

et al. 2020

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Background: Hypovitaminosis D and higher platelet numbers are emerging as cardiovascular risk factors, in particular in obese subjects. Methods: This observational study was aimed at investigating the relationship between platelet number and serum 25-hydroxyvitamin D (25(OH)D) levels in a cohort of individuals affected by overweight and obesity (body mass index (BMI) ≥ 25 Kg/m2). A sample of 341 subjects (248 women, 93 men), aged 18–71 years, taking no medication, was examined. Anthropometric, hormone, metabolic and common routine hematochemical parameters were examined and evaluated in association with platelet count and serum 25(OH)D levels. Results: Platelet numbers were inversely related to age (p < 0.04), 25(OH)D (p < 0.05) and uric acid (p < 0.04) levels, and directly associated with white blood cells (p < 0.01), Thyroid Stimulating Hormone (TSH) (p < 0.04), insulin levels (p < 0.002) and Homeostasis Model Assessment – Insulin Resistance (HOMA-IR) (p < 0.002). We applied statistical regression models to examine the relationship between platelet count (dependent variable) and parameters that had univariate associations with platelet numbers, showing that the association between platelet count and 25(OH)D was not confirmed. Moreover, vitamin D showed a negative independent association with BMI, diastolic blood pressure and serum insulin levels. Conclusions: This study indicates, for the first time, that vitamin D deficiency is associated with a parallel increase in platelet number, suggesting that higher platelet numbers may be one of the possible mechanisms leading to a greater cardiovascular risk in obese subjects. It also shows that vitamin D deficiency, a common condition in obesity, has independent associations with higher BMI, diastolic blood pressure and serum insulin levels.

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Effects of Estrogens on Platelets and Megakaryocytes

Cited by 36 publications

References 64 publications

Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo

Tamoxifen induces hypercoagulation and alterations in ERα and ERβ dependent on breast cancer sub-phenotype ex vivo

Study of association of bleeding and clotting time with blood group among young adults

Hydroxyvitamin D Serum Levels are Negatively Associated with Platelet Number in a Cohort of Subjects Affected by Overweight and Obesity

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