Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis

Ciocan, Corina; Cubero-Leon, Elena; Puinean, Alin M.; Hill, Elizabeth M.; Minier, Christophe; Osada, Makoto; Fenlon, Kate A.; Rotchell, Jeanette M.

doi:10.1016/j.envpol.2010.05.025

Cited by 111 publications

(80 citation statements)

References 44 publications

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“…In zebra mussels, Dreissena polymorpha , the amount of VTG-like protein was reported to be the same in controls of both sexes (Quinn et al, 2004). A 30-day exposure to 2 mM estradiol (over 500 mg/L) did not induce VTG in male nor female E. complanata (Won et al, 2005) while a 10-day exposure to 50 ng/L but not 200 ng/L elevated VTG levels in both sexes of M. edulis (Ciocan et al, 2010). In xenoestrogen-contaminated waters, male M. galloprovincialis , displayed elevated VTG while females did not (Pampanin et al, 2005) yet in D. polymorpha females showed a 40% higher rise in VTG than males (Quinn et al, 2004), and Gagné et al (2001) reported increased VTG in both sexes of E. complanata .…”

Section: Discussionmentioning

confidence: 91%

“…Stimulation of VTG is a classic estrogenic endpoint as this protein is a precursor of egg yolk and normally found only in oviparous females. VTG production by males is a bioassay for estrogenic contamination in many aquatic species including other bivalves (Ciocan et al, 2010; Quinn et al, 2004). The ALP assay used here has been routinely used in E. complanata (Gagné et al, 2011; Bouchard et al, 2009; Gagné and Blaise, 2003) and was validated for this system by showing the expected gender difference in control animals: females had almost 10-fold higher VTG than males.…”

Section: Discussionmentioning

confidence: 99%

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Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Flynn

Wedin

Bonventre

et al. 2013

Journal of Toxicology and Environmental Health, Part A

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The widely used herbicide atrazine (ATR) may have endocrine-associated adverse effects, including on behavior. In this study, 120 adult freshwater mussels, Elliptio complanata, were exposed to ATR at the environmentally-relevant concentrations of 1.5, 15, or 150 μg/L. Burrowing depth was evaluated hourly for 6 hr and at sacrifice animals were sexed by gonad smear. Female controls burrowed overall approximately 30% less than males, the first report of sexual dimorphism in this behavior. Atrazine at 15 μg/L feminized burrowing in both sexes in that exposed animals burrowed 20% less than their same sex controls. Males treated with 1.5 μg /L ATR displayed approximately 20-fold higher vitellogenin (VTG) levels than same sex controls. Higher concentrations of ATR were not associated with increasing effects. A scatterplot showed a weak binomial curve associating low burrowing with high VTG levels. Taken together, these data suggest a non-linear dose-response in behavioral and physiological feminization produced by ATR and support the need to reconsider the widespread use of this compound.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Flynn

Wedin

Bonventre

et al. 2013

Journal of Toxicology and Environmental Health, Part A

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“…Puinean et al (2006) demonstrated that exposure to a high level of E2 fails to induce vtg gene expression in M. edulis. However, the same team observed a significant increase in vtg mRNA expression in M. edulis exposed to a lower dose of E2 at the early stage of gametogenesis (Ciocan et al 2010). All these data except that of Puinean et al (2006) suggest that E2 promotes vitellogenesis in bivalves depending on the dose of the steroids and the gonadal development status.…”

Section: Potential Role In Gametogenesis and Spawningmentioning

confidence: 93%

Variations of estradiol-17β and testosterone levels correlated with gametogenesis in the gonad of Zhikong scallop (Chlamys farreri) during annual reproductive cycle

et al. 2014

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To assess the potential roles of sex steroids in modulating reproductive processes in the Zhikong scallop (Chlamys farreri (Jones and Preston, 1904)), variations in estradiol-17␤ (E2) and testosterone (T) levels in gonads were examined monthly from January to December 2012 by enzyme-linked immunosorbent assay (ELISA). The mean concentrations of E2 and T in gonads ranged from 75.07 to 666.24 pg/g and from 91.09 to 506.28 pg/g, respectively. Concentrations of E2 were significantly higher in ovaries than in testes, while T concentrations were higher in testes than in ovaries during gametogenesis. Concentrations of E2 in females and T in males increased with development and maturation of gonad, attained the highest value before spawning, and decreased rapidly after spawning. A positive correlation between E2 levels and oocyte diameters (r = 0.743, P < 0.05, n = 25) was observed, suggesting that E2 may play a role in oogenesis. These findings indicate that E2 and T, which are highly correlated with the reproductive cycle, may play an important role in sex determination, sex differentiation, gametogenesis, and spawning in C. farreri.Résumé : Afin d'évaluer les rôles possibles des stéroïdes sexuels dans la modulation des processus de reproduction chez le pétoncle farreri (Chlamys farreri (Jones et Preston, 1904)), les variations des concentrations d'estradiol-17␤ (E2) et de testostérone (T) dans les gonades ont été examinées mensuellement de janvier à décembre 2012 par l'analyse immunoenzymatique ELISA. Les concentrations moyennes d'E2 et de T dans les gonades allaient de 75,07 à 666,24 pg/g et de 91,09 à 506,28 pg/g, respectivement.Les concentrations d'E2 étaient significativement plus élevées dans les ovaires que dans les testicules, alors que les concentrations de T étaient plus élevées dans les testicules que dans les ovaires durant la gamétogénèse. Les concentrations d'E2 chez les femelles et de T chez les mâles augmentaient au fil du développement et de la maturation des gonades, atteignaient leurs maximums avant le frai et diminuaient rapidement par la suite. Une corrélation positive observée entre les concentrations d'E2 et le diamètre des oocytes (r = 0,743, P < 0,05, n = 25) donne à penser que l'E2 pourrait jouer un rôle dans l'ovogénèse. Ces résultats indiquent que l'E2 et la T, qui sont fortement corrélés au cycle de reproduction, pourraient jouer un rôle important dans la détermination du sexe, la différenciation sexuelle, la gamétogénèse et le frai chez C. farreri. [Traduit par la Rédaction]

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“…The occurrence of E2 in wastewater and surface water has been investigated in numerous studies (Servos et al 2005;Coleman et al 2010;Yan et al 2012), thus presenting a potential hazard for aquatic species (Rotchell & Ostrander 2003;Caldwell et al 2012). The reported effects of E2 on aquatic species have mainly focused on the endocrine disrupting effects and the mechanisms of action (Osada et al 2003;Maria et al 2008;Ciocan et al 2010). However, some studies have demonstrated that E2 can disrupt non-reproductive endocrine events, namely the stress responses in fish (Teles et al 2005;Maria et al 2008;Moura Costa et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzymes responses of polychaete Perinereis aibuhitensis following chronic exposure to 17β-estradiol

Dong

et al. 2016

Italian Journal of Animal Science

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The occurrence of 17b-estradiol (E2) in the aquatic environment can lead to damage to the reproductive system, along with other adverse effects including oxidative stress, in aquatic organisms. In the present study, Perinereis aibuhitensis were treated with E2 at 0.1, 1, 10, 100 and 1000 lg/L for 205 d, after which the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), and the concentrations of glutathione antioxidant biomarker responses were studied. Weight gain and specific growth rate of P. aibuhitensis were not significantly affected by E2 treatment. Although no significant differences in mortality were observed, the group receiving the highest dose of E2 (1000 lg/L) experienced the highest rate of mortality. Treatment with E2 enhanced the levels of total glutathione (T-GSH), but levels of glutathione reduced (GSH) were significantly decreased by 17.02, 20.55, 23.45, 31.91 and 56.08%, with a concomitant increase in the levels of glutathione disulphide (GSSG) by 148.58, 213.05, 232.62, 294.63 and 306.45%, at 0.1, 1, 10, 100 and 1000 lg/L E2, respectively. The redox ratio of GSH/GSSG was significantly decreased (p < 0.01). The results of this study suggest that long-term exposure of P. aibuhitensis to E2 may inhibit antioxidant enzyme activities, thereby reducing the capacity of its detoxification system. ARTICLE HISTORY

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Effects of estrogen exposure in mussels, Mytilus edulis, at different stages of gametogenesis

Cited by 111 publications

References 44 publications

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Burrowing in the Freshwater MusselElliptio complanatais Sexually Dimorphic and Feminized by Low Levels of Atrazine

Variations of estradiol-17β and testosterone levels correlated with gametogenesis in the gonad of Zhikong scallop (Chlamys farreri) during annual reproductive cycle

Antioxidant enzymes responses of polychaete Perinereis aibuhitensis following chronic exposure to 17β-estradiol

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