Effects of estradiol on in vitro maturation of buffalo and goat oocytes

Maksura, Hurum; Akon, Narsisa; Islam, Nazrul; Akter, Ireen; Modak, Avijit Kumar; Khatun, Asma; Alam, Hasanur; Hashem, MA; Amin, Ruhul; Moniruzzaman, Mohammad

doi:10.1002/rmb2.12350

Cited by 16 publications

(11 citation statements)

References 61 publications

(92 reference statements)

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“…This secretion indicates better cytoplasmic maturation and more advanced folliculogenesis. 9 In this study, it was also known that progesterone level did not affect oocyte maturation rate. Previous studies has shown that progesterone level in ART program participants was one of the variables that have been widely studied to affect the number of oocytes obtained, the maturation level of the oocytes, the fertilization rate, and the success of pregnancy.…”

Section: Discussionmentioning

confidence: 51%

“…8 Other studies on in vitro fertilization of bull and ewe oocytes found that oocytes having richer estradiol environment will have higher nucleus maturation rate than oocytes with low environmental estradiol levels. 9 However, excessive concentration of estradiol Additionally, multivariable analysis revealed that higher estradiol on day-7 was associated with better oocyte maturation rate (p = 0.047). The result of this analysis can be found on Table 3 and 4 would actually inhibit oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

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Estradiol on Day Seven is a Good Predictor for Oocyte Maturation Rate in In Vitro Fertilization Program

Hestiantoro

Putri

Gunardi

et al. 2022

Indones J Obstet Gynecol

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Abstract Objective: to determine which factors influence the rate of oocyte maturation in In Vitro Fertilization (IVF) program. Methods: A retrospective cohort study was conducted using secondary data from IVF participants at the Yasmin Fertility Clinic, Dr. RSUP. Cipto Mangunkusumo, Jakarta, Indonesia during the period January 2019 to December 2020, as recorded in the InaRepromed archive. The variables analyzed were age, body mass index, and hormone levels on day 1, day 7, and day hCG, with oocyte maturation rate as the main outcome. Correlation test was performed between several variables and the level of oocyte maturation rate and followed by multivariate analysis to assess the factors that were closely related to oocyte maturation rate. Results: Data from 52 subjects were collected for the study. Positive correlation was observed between oocyte maturation rate and estradiol on day 7 (r = 0.229), while negative correlation was observed between oocyte maturation rate and progesterone/estradiol ratio on day 7 (r = -0.289) and luteinizing hormone on day 1 (r = -0.265). Multivariate analysis revealed that higher estradiol on day-7 was associated with better oocyte maturation rate (p = 0.047). Conclusion: Higher estradiol level on day 7 was associated with better oocyte maturation rate in IVF. Keywords: Assisted reproductive technology (ART), estradiol, in vitro fertilization (IVF), progesterone. Abstrak Tujuan: untuk menentukan faktor-faktor yang mempengaruhi tingkat pematangan oosit dalam program Fertilisasi InVitro (FIV). Metode: Desain penelitian adalah kohort retrospektif, menggunakan data sekunder peserta bayi tabung Klinik Fertilitas Yasmin, RSUP Dr. Cipto Mangunkusumo, Jakarta, Indonesia selama periode Januari 2019 hingga Desember 2020, yang tercatat di dalam arsip InaRepromed. Variabel yang dianalisis adalah umur, indeks massa tubuh, dan kadar hormon pada hari ke-1, hari ke-7, dan hari ke-hCG, dengan tingkat maturasi oosit sebagai luaran utama. Dilakukan analisis korelasi antara beberapa variabel dengan tingkat maturasi oosit, dan dilanjutkan dengan analisis multivariat untuk menilai faktor-faktor yang berhubungan kuat dengan tingkat maturasi oosit. Hasil: Data dari 52 subjek dikumpulkan untuk penelitian ini. Dijumpai korelasi positif antara tingkat maturasi oosit dan estradiol pada hari ke 7 (r = 0,229), sedangkan korelasi negatif diamati pula antara tingkat maturasi oosit dan rasio progesteron/estradiol pada hari ke 7 (r = -0,289) dan hormon luteinisasi pada hari 1 (r = -0,265). Analisis multivariat mengungkapkan bahwa estradiol yang lebih tinggi pada hari ke-7 dikaitkan dengan tingkat maturasi oosit yang lebih baik (p = 0,047). Kesimpulan: Kadar estradiol yang lebih tinggi pada hari ke 7 dikaitkan dengan tingkat pematangan oosit yang lebih baik pada program FIV. Kata kunci : Teknologi reproduksi berbantu (TRB), estradiol, fertilisasi in vitro (IVF), progesteron.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Estradiol on Day Seven is a Good Predictor for Oocyte Maturation Rate in In Vitro Fertilization Program

Hestiantoro

Putri

Gunardi

et al. 2022

Indones J Obstet Gynecol

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“…E2 is an important steroid hormone that plays a major role in female reproduction (e.g., follicle development, fertilization, pregnancy establishment, and maintenance) [35,36].…”

Section: Discussionmentioning

confidence: 99%

Study on the Pattern of Postpartum Uterine Involution in Dairy Cows

Dai,

Ma,

Guo

et al. 2023

Animals

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Postpartum uterine involution is necessary for the normal reproduction of dairy cows. The study aimed to investigate the pattern of postpartum uterine involution and the impact of parity on uterine involution in Chinese Holstein dairy cows. The diameter of the uterine cervix, pregnant uterine horn, and non-pregnant uterine horn were monitored using a B-mode veterinary ultrasound scanner at 5, 10, 15, 20, 25, 30, 35, and 40 days, respectively, after parturition in both multiparous and primiparous dairy cows. Meanwhile, the concentrations of hydroxyproline, E2, and IGF-1 were detected using ELISA at 5, 10, 15, 20, 30, and 40 d after parturition in both multiparous and primiparous dairy cows. Furthermore, the duration of uterine involution was compared in the multiparous and primiparous dairy cows. The results demonstrated that the diameter of the uterine cervix and the pregnant uterine horn did not decrease any further at 25 days postpartum for both the multiparous cows and the primiparous cows. Hydroxyproline levels gradually decreased with uterine involution; however, there was no significant variation in IGF-1 concentrations during uterine involution in the dairy cows. Although E2 concentrations of the peripheral plasma displayed an upward trend from day 5 to day 15 in the two groups of postpartum cows, there was no significant difference between the two groups during uterine involution. These results suggest that postpartum uterine involution was around 25 days postpartum in both the primiparous dairy cows and the multiparous Chinese Holstein dairy cows. Parity did not affect uterine involution in the postpartum Chinese Holstein dairy cows. The hydroxyproline levels of the peripheral blood may be an indicator of uterine involution in postpartum cows. Nonetheless, IGF-1 and E2 levels of the periphery blood are not associated with uterine involution in Chinese Holstein dairy cows.

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“…Selected COCs were first washed twice with tissue culture medium 199 (TCM-199) supplemented with 25 mM HEPES and 5% fetal bovine serum (FBS), according to Ashour et al (2020). The COCs were washed once in IVM medium consisting of TCM-199 supplemented with 10% (v/v) pre-heated FBS at 56 ºC for 30 min, 10 ng/ mL epidermal growth factor (EGF), 1 μg/mL estradiol (E2), 0.25 mg/mL Na+ pyruvate, and 20 IU pregnant mare serum gonadotropin (PMSG) (Gonaser, 500 IU) according to previous publications (Soto-Heras et al, 2019;Jose et al, 2021;Maksura et al, 2021). The hCG hormone (choriomon®, 5000 IU) was added to IVM medium at various doses (G1: IVM medium without hCG, which was allocated as a control group; G2: IVM medium supplemented with 10 IU of hCG; and G3: IVM medium supplemented with 20 IU of hCG).…”

Section: In Vitro Maturationmentioning

confidence: 99%

“…Accordingly, expanded COCs were considered that clearly showed one or more expanded layers of cumulus cells and those that did not show any observable degree of expansion, as presented in Figure 3. The cumulus expansion rate was calculated as a percentage of total incubated COCs (Maksura et al, 2021).…”

Section: Assessment Of Oocyte Maturationmentioning

confidence: 99%

Gonadotropin supplementation improved in vitro developmental capacity of Egyptian goat oocytes by modulating mitochondrial distribution and utilization

AbdElkhalek,

Soliman,

El Naga

et al. 2023

J. Indonesian Trop. Anim. Agric.

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Goat production in Mediterranean countries is economically and socially significant. The study investigated the impact of hCG supplementation on the in vitro maturation, fertilization, and mitochondrial activity of goat oocytes. In total, 2356 good cumulus-oocyte complexes (COCs) were recovered from 476 freshly collected goat ovaries. Selected oocytes were incubated in IVM medium and supplemented with hCG at varying concentrations depending on the experimental group for 24 hours at 38.5 ° C, 5% CO2 and 95% humidity. The first group (G1) was allocated as a control group, while the G2 and G3 groups were supplemented with 10 and 20 IU/mL hCG, respectively. The maturation rate was calculated using Hoechst 33342 staining in addition to cumulus expansion and the first polar body extrusion rates. The fertilization rate was evaluated. Moreover, mitochondrial activity was assessed using Mito-Tracker fluorescent staining. The results indicated a higher rate (P≤0.05) of nuclear maturation in G3 (82.5%) than in G2 (65.9%) and the control group (64.8%). Furthermore, fertilization rate was significantly improved in G3 (18.7%) compared to G2 (10.6%) and the control group (9.5%). Notably, the percentage of diffuse patterns of mitochondrial distribution increased in G3 (73.3%) compared to G2 (13.3%) and the control group (6.7%). In the same trend, the fluorescent mitochondrial intensity was higher in G3 (95%) and G2 (89.2%) compared to the control group (79.9%). In conclusion, supplementing the IVM medium with 20 IU/mL of hCG improved goat oocytes' maturation and fertilization rates by modulating cytoplasmic distribution and promoting the utilization of oocyte mitochondria.

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Effects of estradiol on in vitro maturation of buffalo and goat oocytes

Cited by 16 publications

References 61 publications

Estradiol on Day Seven is a Good Predictor for Oocyte Maturation Rate in In Vitro Fertilization Program

Estradiol on Day Seven is a Good Predictor for Oocyte Maturation Rate in In Vitro Fertilization Program

Study on the Pattern of Postpartum Uterine Involution in Dairy Cows

Gonadotropin supplementation improved in vitro developmental capacity of Egyptian goat oocytes by modulating mitochondrial distribution and utilization

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