Effects of Enzyme Formulation and Loadings on Conversion of Biomass Pretreated by Leading Technologies

Gupta, Rajesh; Lee, Y. Y.

doi:10.1002/9780470975831.ch13

Cited by 4 publications

(1 citation statement)

References 83 publications

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“…The adsorption capacity and binding affinity vary significantly on feedstock type and Pretreatment methods employed. Generally, pure cellulosic substrates such as Avicel and filter paper have much less enzyme adsorption capacity than pretreated biomass [55]; Avicel adsorbed more cellulase than β-glucosidases [54]. A recent study using corn stover treated with a few leading Pretreatment technologies have revealed that enzyme adsorption is depending on Pretreatment methods with relatively higher cellulase adsorption capacity in high pH Pretreatment approaches (AFEX and lime) [56].…”

Section: Substrates Crystallinity Index (%) At Different Hydrolysis Tmentioning

confidence: 99%

Physicochemical Structural Changes of Cellulosic Substrates during Enzymatic Saccharification

Ragauskas¹

2016

JABB

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Enzymatic hydrolysis represents one of the major steps and barriers in the commercialization process of converting cellulosic substrates into biofuels and other value added products. It is usually achieved by a synergistic action of enzyme mixture typically consisting of multiple enzymes such as glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. Due to the innate biomass recalcitrance, enzymatic hydrolysis normally starts with an initial fast rate of hydrolysis followed by a rapid decrease of rate toward the end of hydrolysis. With majority of literature studies focusing on the effect of key substrate characteristics on the initial rate or final yield of enzymatic hydrolysis, information about physicochemical structural changes of cellulosic substrates during enzymatic hydrolysis is still quite limited. Consequently, what slows down the reaction rate toward the end of hydrolysis is not well understood. This review highlights recent advances in understanding the structural changes of cellulosic substrates during the hydrolysis process, to better understand the fundamental mechanisms of enzymatic hydrolysis.

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Section: Substrates Crystallinity Index (%) At Different Hydrolysis Tmentioning

confidence: 99%

Physicochemical Structural Changes of Cellulosic Substrates during Enzymatic Saccharification

Ragauskas¹

2016

JABB

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A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Mok

Arantes

Saddler

2015

Appl Biochem Biotechnol

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Accurate protein quantification is necessary in many of the steps during the enzymatic hydrolysis of pretreated lignocellulosic biomass, from the fundamental determination of enzyme kinetics to techno-economic assessments, such as the use of enzyme recycling strategies, evaluation of enzyme costs, and the optimization of various process steps. In the work described here, a modified, more rapid ninhydrin-based protein quantification assay was developed to better quantify enzyme levels in the presence of lignocellulosic biomass derived compounds. The addition of sodium borohydride followed by acid hydrolysis at 130 °C greatly reduced interference from monosaccharides and oligosaccharides and decreased the assay time 6-fold. The modified ninhydrin assay was shown to be more accurate as compared to various traditional colorimetric protein assays when commercial cellulase enzyme mixtures were quantified under typical pretreated lignocellulosic biomass enzymatic hydrolysis conditions. The relatively short assay time and microplate-reading capability of the modified assay indicated that the method could likely be used for high-throughput protein determination.

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Pressurized liquid hot water pretreatment and enzymatic hydrolysis of switchgrass aiming at the enhancement of fermentable sugars

2020

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Effects of Enzyme Formulation and Loadings on Conversion of Biomass Pretreated by Leading Technologies

Cited by 4 publications

References 83 publications

Physicochemical Structural Changes of Cellulosic Substrates during Enzymatic Saccharification

Physicochemical Structural Changes of Cellulosic Substrates during Enzymatic Saccharification

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Pressurized liquid hot water pretreatment and enzymatic hydrolysis of switchgrass aiming at the enhancement of fermentable sugars

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