“…A 50:50 mixture of filtered pond water:deionized water was used as the control water and as a base water to prepare the simulated Powell River water. A previous study demonstrated excellent mussel survival in the 50:50 mixture, compared to poor survival in 100% pond water when used in closed exposure systems (Ciparis et al 2015). The target TDS concentration for simulated Powell River water was 950 mg/L, similar to the target concentration for simulated Powell River water (942 mg/L) derived in Ciparis et al (2015).…”
Section: Treatment Preparationsupporting
confidence: 70%
“…A previous study demonstrated excellent mussel survival in the 50:50 mixture, compared to poor survival in 100% pond water when used in closed exposure systems (Ciparis et al 2015). The target TDS concentration for simulated Powell River water was 950 mg/L, similar to the target concentration for simulated Powell River water (942 mg/L) derived in Ciparis et al (2015). This represents recent TDS concentrations measured during low-flow conditions at a Virginia Department of Environmental Quality (VDEQ) longterm monitoring station on the Powell River, at Big Stone Gap, Virginia (6BPOW179.20; for location information see Fig.…”
BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.
“…A 50:50 mixture of filtered pond water:deionized water was used as the control water and as a base water to prepare the simulated Powell River water. A previous study demonstrated excellent mussel survival in the 50:50 mixture, compared to poor survival in 100% pond water when used in closed exposure systems (Ciparis et al 2015). The target TDS concentration for simulated Powell River water was 950 mg/L, similar to the target concentration for simulated Powell River water (942 mg/L) derived in Ciparis et al (2015).…”
Section: Treatment Preparationsupporting
confidence: 70%
“…A previous study demonstrated excellent mussel survival in the 50:50 mixture, compared to poor survival in 100% pond water when used in closed exposure systems (Ciparis et al 2015). The target TDS concentration for simulated Powell River water was 950 mg/L, similar to the target concentration for simulated Powell River water (942 mg/L) derived in Ciparis et al (2015). This represents recent TDS concentrations measured during low-flow conditions at a Virginia Department of Environmental Quality (VDEQ) longterm monitoring station on the Powell River, at Big Stone Gap, Virginia (6BPOW179.20; for location information see Fig.…”
BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.
“…Also, L. siliquoidea is not native to the Powell River. Ciparis et al (2015) tested response by juvenile Villosa iris, a Powell River native mussel, to elevated DS (N900 mg/L) with an ion composition intended to simulate the upper Powell River; no significant effects on growth or survival were found over a 55 day study period. Test concentrations were based on the highest recorded concentrations of major Fig.…”
Section: Relationships Among Mining Water Quality and Mussel Declinesmentioning
“…The Chinese pond mussel (the Eastern Asiatic freshwater clam or swanmussel), bivalve Anodonta woodiana functions as a main criteria required for a bio-indicator organism [18,19]. Earlier studies have revealed the ability of Sinanodonta woodiana to accumulate trace elements and pesticides [20,21], as well as its potential to detect genotoxicity [22]. In addition, these mussels have important ecosystem functions such as particle filtration and processing, nutrient release, and sediment mixing [23].…”
a b s t r a c tPolybrominated diphenyl ethers-47 (PBDE-47) and -209 are significant components of total PBDEs in water and can catalyze the production of reactive oxygen species (ROS) in the organisms. Anti-oxidant enzymes play an important role in scavenging the high level of ROS. In the current study, two fulllength cDNAs of Cu/Zn superoxide dismutase (CuZnSODs) and catalase (CAT) were isolated from freshwater bivalve Anodonta woodiana by rapid amplification of cDNA ends approach and respectively named as AwSOD and AwCAT. The nucleotide sequence of AwSOD cDNA had an open reading frame (ORF) of 465 bp encoding a polypeptide of 155 amino acids in which signature 1 GKHGFHVHEFGDNT and signature 2 GNAGARSACGVI of SODs were observed. Deduced amino acid sequence of AwSOD showed a significant similarity with that of CuZnSODs. AwCAT had an ORF 1536 bp encoding a polypeptide of 512 amino acids which contains a conserved catalytic site motif, and a proximal heme-ligand signature motif of CATs. The time-course expressions of AwSOD and AwCAT in hepatopancreas were measured by quantitative real-time PCR. Expressions of AwSOD and AwCAT showed a significant up-regulation in groups at a low concentration treatment of PBDE-47, a biphasic pattern in groups with a high concentration treatment. Administration of PBDE-209 could result in an up-regulation of AwSOD and AwCAT expressions with time-and dose-dependent matter. These results indicate that up-regulations of AwSOD and AwCAT expression of hepatopancreas of freshwater bivalve A. woodiana contribute to eliminate oxidative stress derived from PBDE-47 and -209 treated.
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