Effects of environmental variables on abundance of ammonia-oxidizing communities in sediments of Luotian River, China

Abstract

Ammonia-oxidizing communities play important functional roles in the nitrification. However, environmental stresses can significantly affect this process by controlling the abundant communities of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) communities. In this study, we examined the abundance variations of ammonia-oxidizing communities using quantitative polymerase chain reaction (qPCR) and terminal-restriction fragment length polymorphism (T-RFLP) in a typical subtropical river, Luotian County, South Dabie Mountains, China. Clone libraries were conducted to evaluate the community structure and abundance of AOA and AOB in sediments. Results showed that Nitrososphaera sp and Nitrosopumilus sp were the most dominant AOA. The abundance of the AOA and AOB amoA gene ranged from 5.28 × 108 gene copies (g-soil−1) to 2.23 × 108 gene copies (g-soil−1) and 5.45 × 108 gene copies (g-soil−1) to 3.30 × 107 gene copies (g-soil−1), respectively. Five environmental variables, namely, ORP, DO, NO${}_{3}^{-}$, Temp, and NH${}_{4}^{+}$ were played a major function in microbial communities of AOA and AOB in sediments. The T-RFLP profiles of AOA showed that 488 and 116 bp T-RFs were dominated. Overall, the results of this study showed that anthropogenic activities andenvironmental stress in rivers can alter the structure and function of microbes in their variable environment.

Structural and functional microbial diversity of sandy soil under cropland and grassland

“…The predominant t-RF 162 in both cropland and grassland soils could be explained by the features of different members of Candidatus Nitrososphaera, that are capable of biofilm formation, detoxification and adhesion, thus they are well adapted to environmental changes (Hammerl et al, 2019;Kerou et al, 2016). Moreover, the members of Thaumarchaeota, for example several species classified in the Nitrosotalea genus, may be adapted to acidic environments with a pH lower than 6.5 (Ginawi et al, 2020), therefore in our study their representatives were found in both tested sandy acidic soils under cropland and grassland. It should be taken into consideration that the assignment of archaeal community was based on databases in silico analysis.…”

“…It should be taken into consideration that the assignment of archaeal community was based on databases in silico analysis. However, the presence of Candidatus Nitrososphaera, Candidatus Nitrosoarchaeum limnia, Nitrosopumilus sp., Candidatus Nitrosotenuis and Candidatus Nitrosotalea were detected in different sediment and soil environments (Hammerl et al, 2019;Cao et al, 2012;Ginawi et al, 2020;Wessén et al, 2011).…”

Biochar Amendment in Vermi-Wetland for Enhancing Nitrification during Excess Sludge Recycling

“…It is apparent that AOB and AOA drive the ammonia-oxidizing process, a rate-limiting phase in nitrification processes [14,15]. Most studies have focused on the dispersion and role of oxidizers of oxidizers (AOA and AOB) in diverse ecosystems [16,17]. For instance, mounted evidence indicates that the AOA dominates the soil ecosystems, displaying a greater quantity and a richer diversity of communities than the AOB [18].…”