Effects of environmental stresses on the physiological characteristics, adhesion ability and pathogen adhesion inhibition of Lactobacillus plantarum KLDS 1.0328

Ma, Jiage; Wang, Wan; Sun, Changbao; Gu, Liya; Liu, Zhijing; Yu, Wenbin; Chen, Lijun; Jiang, Zhanmei; Hou, Juncai

doi:10.1016/j.procbio.2020.02.001

Cited by 27 publications

(20 citation statements)

References 47 publications

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“…The performances of the tested strains appeared to be robust toward the different stressors, in accordance with previous studies, which validated the aptitude of L. plantarum species to tolerate acidic environments, for the intrinsic ability to maintain an internal pH gradient that allows the survival at a much lower external pH [ 26 ]. A recent study [ 38 ], carried out on L. plantarum KLOS 1.0328 strain, showed its greater inclination to tolerate acidic stress rather than osmotic stress, according to a previous work reporting that pH values from 5.0 to 9.0 did not significantly affect the growth of L. plantarum strains isolated from Italian Bella di Cerignola table olives [ 30 ]. However, the same authors asserted that salt concentrations higher than 4% negatively influenced the strain growth.…”

Section: Resultsmentioning

confidence: 99%

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

et al. 2020

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The use of β-glucosidase positive strains, as tailored-starter cultures for table olives fermentation, is a useful biotechnological tool applied to accelerate the debittering process. Nowadays, strains belonging to Lactiplantibacillus plantarum species are selected for their high versatility and tolerance to stress conditions. The present study investigated the effect of different stress factors (pH, temperature and NaCl) on growth and on oleuropein-degrading abilities of selected L. plantarum strains. In addition, the presence of the beta-glucosidase gene was investigated by applying a PCR based approach. Results revealed that, overall, the performances of the tested strains appeared to be robust toward the different stressors. However, the temperature of 16 °C significantly affected the growth performance of the strains both singularly and in combination with other stressing factors since it prolongs the latency phase and reduces the maximum growth rate of strains. Similarly, the oleuropein degradation was mainly affected by the low temperature, especially in presence of low salt content. Despite all strains displayed the ability to reduce the oleuropein content, the beta-glucosidase gene was detected in five out of the nine selected strains, demonstrating that the ability to hydrolyze the oleuropein is not closely related to the presence of beta-glucosidase. Data of the present study suggest that is extremely important to test the technological performances of strains at process conditions in order to achieve a good selection of tailored starter cultures for table olives.

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Section: Resultsmentioning

confidence: 99%

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

et al. 2020

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“…Lactic acid bacteria (LAB) encounter various stress conditions during industrial processes, which are able to produce adaptive responses, and there are multiple self-regulatory mechanisms (Ma et al, 2020). Fructose consumption in foods and beverages has dramatically increased, fructose metabolism by Lactobacillus reuteri alters the metabolic flux in the cells (Oh et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Combined proteomics and transcriptomics analysis of Lactococcus lactis under different culture conditions

Yang

Hong

et al. 2021

Journal of Dairy Science

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During industrial handling, Lactococcus lactis needs to adapt to different culture conditions by regulating its metabolic pathways. Modifying culture conditions may be an important way to control the biomass and functional metabolites of lactic acid bacteria. In this study, we identified the differentially expressed genes and proteins of L. lactis under different culture conditions by integrating transcriptomics and proteomics. We also analyzed the data using a bioinformatic approach to reveal the regulatory mechanisms affected by culture conditions. The transcriptome and proteome studies indicated that different culture conditions (fructose, calcium ion, palmitic acid, low pH) affected gene and protein expressions. The levels of differentially expressed proteins did not significantly correlate with the expression levels of their corresponding genes. Our results highlight the importance of comparative transcriptomics and proteomics analyses. In this study, fructose and pH significantly affected sugar metabolism of L. lactis. When lactose was replaced by fructose, fructokinase expression was promoted, and fructose metabolism was accelerated, whereas starch and sucrose metabolism and galactose metabolism system were inhibited. Low pH may be beneficial to homofermentation of L. lactis, which may also metabolize galactose through the tagatose pathway and the Leloir pathway. Fatty acid metabolism and fatty acid biosynthesis were significantly downregulated under calcium ion and palmitic acid. The purine metabolism was upregulated under fructose treatment and downregulated under palmitic acid treatment.

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“…Such an approach has also been implemented successfully in encapsulating metabolizable sugars and salts, which have been demonstrated to improve probiotics' organoleptic properties and prolong their shelf life [172,173]. Moreover, this approach largely addresses the limited availability of the encapsulated nutrients at the site of action due to unspecific release and fluid infiltration along the transit through the GIT [174].…”

Section: Ph-responsive Matricesmentioning

confidence: 99%

Novel Developments on Stimuli-Responsive Probiotic Encapsulates: From Smart Hydrogels to Nanostructured Platforms

et al. 2022

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Biomaterials engineering and biotechnology have advanced significantly towards probiotic encapsulation with encouraging results in assuring sufficient bioactivity. However, some major challenges remain to be addressed, and these include maintaining stability in different compartments of the gastrointestinal tract (GIT), favoring adhesion only at the site of action, and increasing residence times. An alternative to addressing such challenges is to manufacture encapsulates with stimuli-responsive polymers, such that controlled release is achievable by incorporating moieties that respond to chemical and physical stimuli present along the GIT. This review highlights, therefore, such emerging delivery matrices going from a comprehensive description of addressable stimuli in each GIT compartment to novel synthesis and functionalization techniques to currently employed materials used for probiotic’s encapsulation and achieving multi-modal delivery and multi-stimuli responses. Next, we explored the routes for encapsulates design to enhance their performance in terms of degradation kinetics, adsorption, and mucus and gut microbiome interactions. Finally, we present the clinical perspectives of implementing novel probiotics and the challenges to assure scalability and cost-effectiveness, prerequisites for an eventual niche market penetration.

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Effects of environmental stresses on the physiological characteristics, adhesion ability and pathogen adhesion inhibition of Lactobacillus plantarum KLDS 1.0328

Cited by 27 publications

References 47 publications

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

Effects of Different Stress Parameters on Growth and on Oleuropein-Degrading Abilities of Lactiplantibacillus plantarum Strains Selected as Tailored Starter Cultures for Naturally Table Olives

Combined proteomics and transcriptomics analysis of Lactococcus lactis under different culture conditions

Novel Developments on Stimuli-Responsive Probiotic Encapsulates: From Smart Hydrogels to Nanostructured Platforms

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