Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan

David, Mariana Rocha; Santos, Lilha Maria Barbosa dos; Vicente, Ana Carolina Paulo; Maciel-de-Freitas, Rafael

doi:10.1590/0074-02760160238

Cited by 85 publications

(79 citation statements)

References 34 publications

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“…The overall uniqueness of the laboratory samples was also found to be pronounced in our samples contrary to other studies that find high degree of similarity between field and laboratory samples (Wang et al, 2011;David et al, 2016). We found all bacterial community compositions from each field location/season different from the laboratory samples via pairwise PERMANOVA (Figs 4, S6).…”

Section: Discussioncontrasting

confidence: 95%

Investigation of the seasonal microbiome of Anopheles coluzzii in Mali

Krajacich

Huestis

Dao

et al. 2017

Preprint

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The poorly understood mechanisms of the seasonal maintenance of Anopheles spp.mosquitoes through the dry season in Africa remain a critical gap in our knowledge of Plasmodium disease transmission. While it is thought that adult mosquitoes remain in a dormant state throughout this seven-month dry season, the nature of this state remains unknown and has largely not been recapitulated in laboratory settings. To elucidate possible life history traits allowing for this phenotype, the spatiotemporal change in the microbiome of mosquitoes in the dry and wet seasons in Mali was analyzed by sequencing the 16S ribosome bacterial region in whole bodies of adult mosquitoes collected from two locations with varying water availability. These locations were a village near the Niger River with year-round water sources (N'Gabakoro, "Riparian"), and an area closer to the Sahara with highly seasonal breeding sites (Thierola Area, "Sahelian"). The 16S bacterial data consisted of 2057 unique sequence variants in 426 genera across 184 families. With these, we found several compositional differences that were seasonally and spatially linked. Counter to our initial hypothesis, there was a more pronounced seasonal difference in the bacterial microbiome in the Riparian than Sahelian area. These major seasonal shifts were in Ralstonia, Sphingorhabdus, and Duganella spp. bacteria that are usually soil and water-associated, indicating that these changes may be from bacteria acquired in the larval environment, rather than during adulthood. In the Sahelian dry season mosquitoes, there was a unique intracellular bacteria, Anaplasma, which likely was acquired through non-human blood feeding. Coupled with this finding, cytochrome B analysis showed a greater heterogeneity in host choice of An. coluzzii independent of season in the Thierola area compared to N'Gabakoro (77.5% vs. 94.6% human-origin blood meal, respectively), which may indicate a relaxation of anthropophily in some locations. This study highlights the diversity present in the bacterial composition of individual mosquitoes, characterizes PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.3391v1 | CC0 Open Access |

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Section: Discussioncontrasting

confidence: 95%

Investigation of the seasonal microbiome of Anopheles coluzzii in Mali

Krajacich

Huestis

Dao

et al. 2017

Preprint

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“…When abundant microbes were classified at higher taxonomic levels, our analysis found 22 families, with Enterobacteriaceae being the most common when disregarding Wolbachia. Bacteria found in mosquitoes were classified into five phyla with bacteria in the phylum Proteobacteria most prevalent in the microbiome, which is consistent with previous studies ( Osei-Poku et al, 2012 ; David et al, 2016 ; Muturi et al, 2016b ; Audsley et al, 2017a ), although other reports indicated Bacteroidetes or Acinetobacter phyla can be a major component of the microbiome ( Coon et al, 2014 , 2016b ; Minard et al, 2014 , 2015 ).…”

Section: Resultssupporting

confidence: 90%

Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors

et al. 2018

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Microbial interactions are an underappreciated force in shaping insect microbiome communities. Although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence and co-exclusion interactions within the microbiome. To characterize these patterns in mosquitoes, we sequenced the bacterial microbiome of Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus caught in the field or reared in the laboratory and used these data to generate interaction networks. For collections, we used traps that attracted host-seeking or ovipositing female mosquitoes to determine how physiological state affects the microbiome under field conditions. Interestingly, we saw few differences in species richness or microbiome community structure in mosquitoes caught in either trap. Co-occurrence and co-exclusion analysis identified 116 pairwise interactions substantially increasing the list of bacterial interactions observed in mosquitoes. Networks generated from the microbiome of Ae. aegypti often included highly interconnected hub bacteria. There were several instances where co-occurring bacteria co-excluded a third taxa, suggesting the existence of tripartite relationships. Several associations were observed in multiple species or in field and laboratory-reared mosquitoes indicating these associations are robust and not influenced by environmental or host factors. To demonstrate that microbial interactions can influence colonization of the host, we administered symbionts to Ae. aegypti larvae that either possessed or lacked their resident microbiota. We found that the presence of resident microbiota can inhibit colonization of particular bacterial taxa. Our results highlight that microbial interactions in mosquitoes are complex and influence microbiome composition.

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“…albopictus at immature stages (larvae, pupae) while inhabiting water with different ampicillin concentrations but also the variations in the microbiome composition of unfed adult females hatched from the aforementioned pupae. The predominance of Proteobacteria in most immature stage groups was consistent with the findings of previous studies (Audsley et al, 2017; David, Santos, Vicente, & Maciel‐de‐Freitas, 2016; Muturi, Kim, Bara, Bach, & Siddappaji, 2016; Osei‐Poku et al, 2012). However, divergence was observed in the dominant phylum between adult females that were originating from immature stages in the ampicillin‐treated groups (A1–A4) and those originating from immature stages in the control group (A5) (Figure 1).…”

Section: Discussionsupporting

confidence: 90%

Variation in the microbiota across different developmental stages of Aedes albopictus is affected by ampicillin exposure

et al. 2020

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The microbiota plays an important role in the growth of mosquitoes and the transmission of mosquito‐borne pathogens. The effects of changes in aquatic habitats in which mosquitoes live, as one of the major factors closely associated with the microbial communities of mosquitoes, on the microbiota of different developmental stages remain to be elucidated. Here, we compared the microbiota of larvae and pupae of Aedes albopictus exposed to different ampicillin concentrations and investigated the bacterial composition of adult females. The results demonstrate that the microbial community differed substantially between developmental stages and that samples of the same stages shared similarities, whereas differences were observed between adult females. Based on all observations, we hypothesize that the use of ampicillin caused dysbiosis rather than excluding bacteria from mosquitoes and that the disturbing effect of ampicillin was obvious in adults. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that most of the bacteria identified in this study were significantly associated with metabolism. Taken together, our results indicate that ampicillin can change the abundance of bacteria, while microbial communities of Ae. albopictus showed obvious stage‐specific characteristics. Further investigations are needed to characterize specific bacterial components that are affected by ampicillin exposure and to quantify their functions, thereby providing a better understanding of the influence of antibiotics on microbial communities at different life stages.

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Effects of environment, dietary regime and ageing on the dengue vector microbiota: evidence of a core microbiota throughout Aedes aegypti lifespan

Cited by 85 publications

References 34 publications

Investigation of the seasonal microbiome of Anopheles coluzzii in Mali

Investigation of the seasonal microbiome of Anopheles coluzzii in Mali

Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors

Variation in the microbiota across different developmental stages of Aedes albopictus is affected by ampicillin exposure

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