Effects of enteral nutrition with different energy supplies on metabolic changes and organ damage in burned rats

Abstract

Background Enteral nutrition (EN) is an important treatment for burn patients. However, severe gastrointestinal damage caused by major burns often leads to EN intolerance. Trophic EN solves this problem basically, but how to transition from trophic EN to standard EN smoothly is still a challenge in burn clinical nutrition. The aim of this study is to investigate the effects of EN with different energy supplies on metabolic changes, organ damage and prognosis in burned rats. Methods Different feeding regimens were designed based on the continuous monitoring of resting energy expenditure in rats. Thirty-two Sprague–Dawley rats were randomly divided into a normal control group, burn +50% REE group, burn +75% REE group and burn +100% REE group. At the end of a nutritional treatment cycle (14th day), nuclear magnetic resonance spectroscopy, blood biochemistry analysis and quantification of subscab bacteria were performed to explore the differences in metabolic changes, degrees of organ damage and prognoses between the groups. Results Sixteen metabolites involving seven metabolic pathways were identified from the different energy supply groups. After burn injury, resting energy consumption and body weight loss increased obviously. Meanwhile, weight loss was inversely related to energy supply. The greatest changes in the degree of organ damage, the level of plasma proteins, lipids and endotoxins, as well as the quantification of subscab bacteria were observed in the 50% REE group, followed by the 75 and 100% groups. Conclusions Achieving an early balance between energy supply and expenditure is conducive to mitigating metabolic disorders and improving prognosis after burn injury.

1H-nuclear magnetic resonance analysis reveals dynamic changes in the metabolic profile of patients with severe burns

“…However, the intricacies of the biochemical and metabolic processes responsible for this phenomenon have not been fully elucidated. Metabolomics has been utilized to investigate metabolic changes associated with trauma [ 9 , 10 ], burn injuries [ 11 , 12 ] and sepsis [ 13 ]. Nevertheless, there are few metabolomics studies that profile changes in the metabolism of patients with severe burns [ 14 ].…”