Effects of Enflurane and Isoflurane on Hepatic and Renal Circulations in Chronically Instrumented Dogs

Bernard, Jean-Marc; Doursout, Marie–Françoise; Wouters, Patrick; Hartley, Craig J.; Cohen, Marvin; Merin, Robert G.; Chelly, Jacques E.

doi:10.1097/00000542-199102000-00016

Cited by 20 publications

(3 citation statements)

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“…Another limitation to our study is the lack of hemodynamic stability during the microsphere injection procedure and microsphere circulation. The animals are anesthetized using isoflurane, which is known to affect organ perfusion (Bernard et al, 1991) and cardiovascular dynamics (Bernard et al, 1990). Finally, without the use of assisted ventilation, this is a terminal procedure and does not allow for the serial injection of microspheres for the evaluation of longitudinal change in skeletal perfusion.…”

Section: Discussionmentioning

confidence: 99%

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

2017

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Disturbances in bone blood flow have been shown to have deleterious effects on bone properties yet there remain many unanswered questions about skeletal perfusion in health and disease, partially due to the complexity of measurement methodologies. The goal of this study was use fluorescent microspheres in rats to assess regional bone perfusion by adapting mouse-specific fluorescent microsphere protocol. Ten fifteen-week old Sprague Dawley rats were injected with fluorescent microspheres either via cardiac injection (n = 5) or via tail vein injection (n = 5). Femora and tibiae were harvested and processed to determine tissue fluorescence density (TFD) which is proportional to the number of spheres trapped in the tissue capillaries. Right and left total femoral TFD (2.77 ± 0.38 and 2.70 ± 0.24, respectively) and right and left tibial TFD (1.11 ± 0.26 and 1.08 ± 0.34, respectively) displayed bilateral symmetry in flow when assessed in cardiac injected animals. Partitioning of the bone perfusion into three segments along the length of the bone showed the distal femur and proximal tibia received the greatest amount of perfusion within their respective bones. Tail vein injection resulted in unacceptably low TFD levels in the tibia from 4 of the 5 animals. In conclusion this report demonstrates the viability of cardiac injection of fluorescent microspheres to assess bone tissue perfusion in rats.

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Section: Discussionmentioning

confidence: 99%

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

2017

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“…During reperfusion there was fluctuation in portal flow in all three groups. In the sham group, the trend in portal flow was for a gradual increase during reperfusion, which might have been an effect of isoflurane, which increases hepatic blood flow and oxygen supply despite lowering systemic arterial pressure 26,27 .…”

Section: Discussionmentioning

confidence: 99%

Continuous infusion ofN-acetylcysteine reduces liver warm ischaemia–reperfusion injury

Glantzounis

Yang

Koti

et al. 2004

British Journal of Surgery

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“…A recovery standard was not utilized in order to ensure that sample is not lost during processing. The animals are anesthetized using isoflurane, which is known to affect organ perfusion ( Bernard et al, 1991 ) and cardiovascular dynamics ( Bernard et al, 1990 ). Without the use of assisted ventilation, the open-chest cardiac injection of microspheres is performed under diminishing physiologic hemodynamic, as well as hypoxic, conditions.…”

Section: Discussionmentioning

confidence: 99%

Skeletal vascular perfusion is altered in chronic kidney disease

et al. 2018

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Patients with chronic kidney disease (CKD) are at an alarming risk of cardiovascular disease and fracture-associated mortality. CKD has been shown to have negative effects on vascular reactivity and organ perfusion. Although alterations in bone blood flow are linked to dysregulation of bone remodeling and mass in multiple conditions, changes to skeletal perfusion in the setting of CKD have not been explored. The goal of this study was to establish the effect of CKD on skeletal perfusion in a rat model of CKD. In two experiments with endpoints at 30 and 35 weeks of age, respectively, normal (NL) and Cy/+ (CKD) animals (n = 6/group) underwent in vivo intra-cardiac fluorescent microsphere injection to assess bone tissue perfusion. These two separate time points aimed to describe skeletal perfusion at 30 and 35 weeks based on previous studies demonstrating significant progression of hyperparthyroid bone disease during this timeframe. CKD animals had blood urea nitrogen (BUN) levels significantly higher than NL at both 30 and 35 weeks. At 30 weeks, perfusion was significantly higher in the femoral cortex (+259%, p < 0.05) but not in the tibial cortex (+140%, p = 0.11) of CKD animals relative to NL littermates. Isolated tibial marrow perfusion at 30 weeks showed a trend toward being higher (+183%, p = 0.08) in CKD. At 35 weeks, perfusion was significantly higher in both the femoral cortex (+173%, p < 0.05) and the tibial cortex (+241%, p < 0.05) in CKD animals when compared to their normal littermates. Isolated tibial marrow perfusion (−57%, p <0.05) and vertebral body perfusion (−71%, p <0.05) were lower in CKD animals. The current study demonstrates two novel findings regarding bone perfusion in an animal model of high turnover CKD. First, cortical bone perfusion in CKD animals is higher than in normal animals. Second, alterations in bone marrow perfision differed among the stages of CKD and were distinct from perfusion to the cortical bone. Determining whether these changes in bone perfusion are drivers, propagators, or consequences of skeletal deterioration in CKD will necessitate further work.

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Effects of Enflurane and Isoflurane on Hepatic and Renal Circulations in Chronically Instrumented Dogs

Cited by 20 publications

References 0 publications

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

Assessment of regional bone tissue perfusion in rats using fluorescent microspheres

Continuous infusion ofN-acetylcysteine reduces liver warm ischaemia–reperfusion injury

Skeletal vascular perfusion is altered in chronic kidney disease

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