Effects of endophytic Streptomyces and mineral nitrogen on Lucerne (Medicago sativa L.) growth and its symbiosis with rhizobia

Le, Xuyen H.; Ballard, Ross; Franco, Christopher M. M.

doi:10.1007/s11104-015-2704-1

Cited by 15 publications

(7 citation statements)

References 39 publications

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“…The N content in shoots was determined using the method described by Le et al (2016a). The shoots were dried to constant weight at 60 °C for 48 h and ground to about 1 mm in size using a mortar and pestle, then sent to Australian Precision Ag Laboratory (APAL, Hindmarsh, South Australia, www.…”

Section: Plant N Content Analysismentioning

confidence: 99%

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Barnett

et al. 2022

Plant Soil

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Purpose The effects of endophytic actinobacterial strains, Microbispora sp. CP56, Actinomadura sp. CP84B, Streptomyces spp. CP200B and CP21A, on the chickpea-Mesorhizobium symbiosis, were investigated in planta, with the aim of revealing the underlying mechanisms of action. Methods The actinobacterial endophytes were co-inoculated with Mesorhizobium ciceri onto chickpea seedlings to study the effect on plant growth parameters, nodulation development and grain yield. The role of actinobacterial exudates on rhizobial growth was investigated, as was the role of root exudates of actinobacteria-colonized plants on the expression of rhizobial nod factors and biofilm formation. Changes in expression of plant flavonoids and bacterial N-fixation genes resulting from actinobacterial co-inoculation were assessed using qPCR. Results Application of actinobacterial endophytes, together with M. ciceri, showed growth promotion of chickpea with an increase in root nodule number and weight. Enhanced nodulation was accompanied by increases in total plant nitrogen, larger total plant weight and a 2–3-fold increase in grain yield. Factors associated with this tripartite symbiosis are promotion of rhizobial growth, earlier nodule formation, increased secondary root formation, up-regulated expression of genes related to flavonoid synthesis and nif genes. In addition, exudates of chickpea roots colonised with actinobacteria increased nodulation-related biological processes - rhizobial chemotaxis, biofilm formation and nod gene expression. Conclusion These endophytic actinobacteria positively affect many aspects of the chickpea-Mesorhizobium symbiosis resulting in increases in grain yield. Similar improvements recorded in chickpea growing in potted field soils, shows the potential to enhance chickpea production in the field.

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Section: Plant N Content Analysismentioning

confidence: 99%

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Barnett

et al. 2022

Plant Soil

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“…It is the first collection of endophytes from the whole Alfalfa plant. In the past research, the root of Alfalfa was the focus, which had the limitation of the endophytes research [17,18].…”

Section: Discussionmentioning

confidence: 99%

Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

Wu¹,

Mei²,

Chen³

et al. 2020

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Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.

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“…), thereby providing significant fixed-nitrogen inputs into agricultural systems ( 2 ). Endophytic actinobacteria have also been isolated from lucerne roots and have been shown to promote root nodulation and contribute to increased symbiotic nitrogen fixation and improved legume growth ( 3 , 4 ). The phylum Actinobacteria is important in plant (roots) interactions with other ecosystem components, with Streptomyces being a widely recognized genus in this phylum.…”

Section: Genome Announcementmentioning

confidence: 99%

“…are ubiquitous and have been isolated from soil (rhizosphere and nonrhizosphere) and within different parts of plants, such as leaves, stems, and roots ( 5 ). Their presence in legume roots as endophytes has been shown to promote nodulation and improve plant growth and N content ( 4 , 6 , 7 ). In addition, members of this genus have been used as biocontrol agents and to enhance drought resistance in crops ( 8 , 9 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequences of the Endophytic Streptomyces sp. Strains LUP30 and LUP47B, Isolated from Lucerne Plants

Franco

Adetutu

et al. 2017

Genome Announc

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Effects of endophytic Streptomyces and mineral nitrogen on Lucerne (Medicago sativa L.) growth and its symbiosis with rhizobia

Cited by 15 publications

References 39 publications

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Revealing the underlying mechanisms mediated by endophytic actinobacteria to enhance the rhizobia - chickpea (Cicer arietinum L.) symbiosis

Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

Complete Genome Sequences of the Endophytic Streptomyces sp. Strains LUP30 and LUP47B, Isolated from Lucerne Plants

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