Effects of endocrine disrupting chemicals on estrogen receptor alpha and heat shock protein 60 gene expression in primary cultures of loggerhead sea turtle (Caretta caretta) erythrocytes

Cocci, Paolo; Capriotti, Martina; Mosconi, Gilberto; Palermo, Francesco Alessandro

doi:10.1016/j.envres.2017.07.024

Cited by 13 publications

(6 citation statements)

References 75 publications

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“…Despite this evidence, the long-term effects of EDCs exposure were not deeply investigated. EDCs exert their actions triggering mainly genomic mechanisms but also promoting non-genomic actions [7][8][9], through the binding to several hormone receptors, including thyroid and, especially, steroid receptors, mainly estrogen (ER) or androgen (AR) receptors, compromising reproductive system and negatively affect the fetal and neonatal development and physiology [10]. Despite several evidences in literature on the potential effects of EDCs on human reproduction, the molecular mechanisms underlying these effects are not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Bisphenol A: an emerging threat to female fertility

Pivonello

Muscogiuri

Nardone

et al. 2020

Reprod Biol Endocrinol

168

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Bisphenol-A (BPA) has been reported to be associated to female infertility. Indeed, BPA has been found to be more frequently detected in infertile women thus leading to hypothesize a possible effect of BPA on natural conception and spontaneous fecundity. In addition, in procedures of medically assisted reproduction BPA exposure has been found to be negatively associated with peak serum estradiol levels during gonadotropin stimulation, number of retrieved oocytes, number of normally fertilized oocytes and implantation. BPA deleterious effects are more critical during perinatal exposure, causing dysregulation of hypothalamic-pituitary-ovarian axis in pups and adults, with a precocious maturation of the axis through a damage of GnRH pulsatility, gonadotropin signaling and sex steroid hormone production. Further, BPA exposure during early lifestage may have a transgenerational effect predisposing the subsequent generations to the risk of developing BPA related disease. Experimental studies suggested that prenatal, perinatal and postnatal exposure to BPA can impair several steps of ovarian development, induce ovarian morphology rearrangement and impair ovarian function, particularly folliculogenesis, as well as can impair uterus morphology and function, in female adult animal and offspring. Finally, studies carried out in animal models have been reported the occurrence of endometriosis-like lesions after BPA exposure. Moreover, BPA exposure has been described to encourage the genesis of PCOS-like abnormalities through the impairment of the secretion of sex hormones affecting ovarian morphology and functions, particularly folliculogenesis. The current manuscript summarizes the evidence regarding the association between BPA exposure and female infertility, reviewing both clinical and preclinical studies.

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Section: Introductionmentioning

confidence: 99%

Bisphenol A: an emerging threat to female fertility

Pivonello

Muscogiuri

Nardone

et al. 2020

Reprod Biol Endocrinol

168

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“…The study by Ivanov et al [ 119 ] proved the ability of the ex vivo system to respond to ionizing radiations with formation of chromosome aberrations, a genotoxicity endpoint, while the studies by Fischer et al [ 120 ] and by Krakauer et al [ 121 ] demonstrated the modulation of cytokines secretion in whole blood after exposure to different compounds. Cocci et al [ 20 ] used erythrocyte cultures from C. caretta exposed to a phthalate, the DiDP. In the present study the cell viability of all blood samples indicated a good condition for the feasibility of the biomarker analysis, showing values higher than 85%.…”

Section: Resultsmentioning

confidence: 99%

“…To date, few studies have been published that apply in vitro methodologies to C. caretta and other sea turtles, mostly Chelonia mydas [ 19 , 20 , 21 , 22 ]. They are essentially based on the development of cell lines from various tissues, on which some organic and more often inorganic contaminants, e.g.…”

Section: Introductionmentioning

confidence: 99%

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

Bianchi

Casini

Vantaggiato

et al. 2022

IJERPH

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The principal aim of the present study was to develop and apply novel ex vivo tests as an alternative to cell cultures able to evaluate the possible effects of emerging and legacy contaminants in Caretta caretta. To this end, we performed ex vivo experiments on non-invasively collected whole-blood and skin-biopsy slices treated with chrysene, MEHP, or PBDE-47. Blood samples were tested by oxidative stress (TAS), immune system (respiratory burst, lysozyme, and complement system), and genotoxicity (ENA assay) biomarkers, and genotoxic and immune system effects were observed. Skin slices were analyzed by applying a 2D-PAGE/MS proteomic approach, and specific contaminant signatures were delineated on the skin proteomic profile. These reflect biochemical effects induced by each treatment and allowed to identify glutathione S-transferase P, peptidyl-prolyl cis-trans isomerase A, mimecan, and protein S100-A6 as potential biomarkers of the health-threatening impact the texted toxicants have on C. caretta. Obtained results confirm the suitability of the ex vivo system and indicate the potential risk the loggerhead sea turtle is undergoing in the natural environment. In conclusion, this work proved the relevance that the applied ex vivo models may have in testing the toxicity of other compounds and mixtures and in biomarker discovery.

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“…Cell viability was also quantified using the Alamar Blue™ assay reagent (Thermo Scientific, Waltham, MA, USA) as described by Cocci et al [23] and following manufacturer's specifications. The incubation medium was removed after 24, 48, 72, 96 h of treatment, replaced with a fresh culture medium containing AB reagent at a final concentration of 10%, and incubated for an additional 1 h. The absorbance was monitored at 570/600 nm using a microplate reader.…”

Section: Alamar Blue Assaymentioning

confidence: 99%

“…Estrogen-like EDCs (xenoestrogens) have the capability to bind to the estrogen receptors (ERs), mimicking the female steroid hormone, 17β-estradiol (E2), and thus activating intracellular signaling pathways. Activation of the ER-mediated signaling pathway has been extensively studied in several models, particularly fish in which feminization has been considered a direct result of xenoestrogen contamination [19][20][21][22][23]. In this regard, the ER-induced hepatic vitellogenin (Vtg) production is typically used to confirm exposure to estrogenic compounds in male fish [24,25].…”

Section: Introductionmentioning

confidence: 99%

An In Silico and In Vitro Study for Investigating Estrogenic Endocrine Effects of Emerging Persistent Pollutants Using Primary Hepatocytes from Grey Mullet (Mugil cephalus)

2021

Self Cite

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There is growing concern about the environmentally relevant concentrations of new emerging persistent organic pollutants, such as perfluorinated compounds and pharmaceuticals, which are found to bioaccumulate in aquatic organisms at concentrations suspected to cause reproductive toxicity due to the activation of estrogen receptor (ER) α and β subtypes. Here, we use a combined in silico and in vitro approach to evaluate the impact of perfluorononanoic acid (PFNA) and Enalapril (ENA) on grey mullet (Mugil cephalus) hepatic estrogen signaling pathway. ENA had weak agonist activity on ERα while PFNA showed moderate to high agonist binding to both ERs. According to these effects, hepatocytes incubation for 48 h to PFNA resulted in a concentration-dependent upregulation of ER and vitellogenin gene expression profiles, whereas only a small increase was observed in ERα mRNA levels for the highest ENA concentration. These data suggest a structure–activity relationship between hepatic ERs and these emerging pollutants.

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Effects of endocrine disrupting chemicals on estrogen receptor alpha and heat shock protein 60 gene expression in primary cultures of loggerhead sea turtle (Caretta caretta) erythrocytes

Cited by 13 publications

References 75 publications

Bisphenol A: an emerging threat to female fertility

Bisphenol A: an emerging threat to female fertility

A Novel Ex Vivo Approach Based on Proteomics and Biomarkers to Evaluate the Effects of Chrysene, MEHP, and PBDE-47 on Loggerhead Sea Turtles (Caretta caretta)

An In Silico and In Vitro Study for Investigating Estrogenic Endocrine Effects of Emerging Persistent Pollutants Using Primary Hepatocytes from Grey Mullet (Mugil cephalus)

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