Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy

Ikehara, Toshitaka; Yamaguchi, Hisao; Hosokawa, Keiko; Miyamoto, Hiroshi; Aizawa, Katsuo

doi:10.1002/bem.10120

Cited by 31 publications

(18 citation statements)

References 51 publications

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“…Santoro et al [1997] showed that extremely low frequency (ELF) MFs influence physiological processes in different organisms, such as plasma membrane structure modification and initiation of signal cascade pathways interference. Cell membrane morphology modification by ELF was again reaffirmed by Ikehara et al [2003], who found that exposure to the ELF MF has reversible effects on N─H inplane bending and C─N stretching vibrations of peptide linkages, and changes the secondary structures of a-helix and b-sheet in cell membrane proteins.…”

Section: Introductionmentioning

confidence: 78%

Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field

Cheng

Rajjoub

Shashurin

et al. 2016

Bioelectromagnetics

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It has been reported since late 1970 that magnetic field interacts strongly with biological systems. Cold atmospheric plasma (CAP) has also been widely studied over the past few decades in physics, biology, and medicine. In this study, we propose a novel idea to combine static magnetic field (SMF) with CAP as a tool for cancer therapy. Breast cancer cells and wild type fibroblasts were cultured in 96-well plates and treated by CAP with or without SMF. Breast cancer cells MDA-MB-231 showed a significant decrease in viability after direct plasma treatment with SMF (compared to only plasma treatment). In addition, cancer cells treated by the CAP-SMF-activated medium (indirect treatment) also showed viability decrease but was slightly weaker than the direct plasma-SMF treatment. By integrating the use of SMF and CAP, we were able to discover their advantages that have yet to be utilized. Bioelectromagnetics. 38:53-62, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 78%

Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field

Cheng

Rajjoub

Shashurin

et al. 2016

Bioelectromagnetics

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“…Previous studies show that exposure to a static magnetic field resulted in an increased Ca +2 level in numerous cells, including macrophages [50], astrocytoma cells [51], [52], and chromaffine cells [53]. This increase of [Ca +2 ]i may be mediated by the change of the structure and dynamic properties of lipid membrane [54], [55], membrane potential and cell surface charge [56], protein structure [57], [58], and intramembrane proteins distribution [59]. [Ca +2 ]i is mainly increased by either stimulation of Ca +2 influx mediated by the Ca +2 channel of the cell membrane or Ca +2 released from intracellular Ca +2 stored when a static magnetic field is applied [60], [61].…”

Section: Discussionmentioning

confidence: 98%

Magnetoreception System in Honeybees (Apis mellifera)

Hsu

et al. 2007

PLoS ONE

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Honeybees (Apis mellifera) undergo iron biomineralization, providing the basis for magnetoreception. We showed earlier the presence of superparamagnetic magnetite in iron granules formed in honeybees, and subscribed to the notion that external magnetic fields may cause expansion or contraction of the superparamagnetic particles in an orientation-specific manner, relaying the signal via cytoskeleton (Hsu and Li 1994). In this study, we established a size-density purification procedure, with which quantitative amount of iron granules was obtained from honey bee trophocytes and characterized; the density of iron granules was determined to be 1.25 g/cm3. While we confirmed the presence of superparamagnetic magnetite in the iron granules, we observed changes in the size of the magnetic granules in the trophycytes upon applying additional magnetic field to the cells. A concomitant release of calcium ion was observed by confocal microscope. This size fluctuation triggered the increase of intracellular Ca+2 , which was inhibited by colchicines and latrunculin B, known to be blockers for microtubule and microfilament syntheses, respectively. The associated cytoskeleton may thus relay the magnetosignal, initiating a neural response. A model for the mechanism of magnetoreception in honeybees is proposed, which may be applicable to most, if not all, magnetotactic organisms.

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“…This molecular rotation within the membrane matrix influences the imbedded ion channels, most likely by producing some degree of deformity of their intramembranous segment, i.e., the part of the structure which is responsible for activation [22,23]. ELF-PMF exposure also influences the lipid components of the cellular membrane, and the N-H in-plane bending and C-N stretching vibrations of peptide linkages, modifying the secondary structures of α-helix and β-sheet contents and producing unfolding process in cell membrane proteins of Hela (41.7-43.6 mT, 50 Hz, ELF-PMF, for up to 1 min) and differentiated SH-SY5Y cells (0.81-1.41, 50 Hz, ELF-PMF, for up to 4 h) [17,24]. Thus, MF affects membrane proteins either directly, or indirectly through the neighbouring phospholipids [17,22,23].…”

Section: Alteration Of Building Moleculesmentioning

confidence: 99%

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

Vergallo

Dini

2018

Sustainability

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Abstract:Moderate static magnetic fields (SMFs) are generated from sources such as new-generation electric trams and trains, electric arc welding, and magnetic resonance imaging (MRI) devices, as well as during the industrial production of aluminium, while extremely low frequency pulsed magnetic fields (ELF-PMFs) are produced by house power installations, household appliances, and high voltages transmission lines. Moderate SMFs and ELF-PMFs with magnetic flux densities (B) in the range of 1-60 mT and frequencies (f ) up to 50 Hz are common MF exposure sources for the population. Even though humans are continually exposed to these MFs, to date no definitive endpoint has been drawn about their safety. In this review, the state of knowledge about the biological effects induced by these MFs on different cell types will be addressed. In our own observation, the putative modulation of Ca 2+ /H + and Na + /H + plasma membrane antiporters of human peripheral blood lymphocytes (PBLs) was found to occur after a 24 h exposure to a 6 mT SMF, and the bystander effect observed on U937 cells cultivated for up to 6 h in the conditioned medium harvested from human PBLs previously exposed for 24 h to the same MF (secondary necrosis induction) will be also herein discussed.

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Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy

Cited by 31 publications

References 51 publications

Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field

Enhancing cold atmospheric plasma treatment of cancer cells by static magnetic field

Magnetoreception System in Honeybees (Apis mellifera)

Comparative Analysis of Biological Effects Induced on Different Cell Types by Magnetic Fields with Magnetic Flux Densities in the Range of 1–60 mT and Frequencies up to 50 Hz

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