Effects of elevated temperature on meiotic chromosome synapsis in Allium ursinum

Loidl, Josef

doi:10.1007/bf00295029

Cited by 61 publications

(56 citation statements)

References 48 publications

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“…From the present study and the work presented by earlier workers on this aspect especially Sjödin (1970), Gottschalk and Kaul (1980a, b), Kaul and Murthy (1985), Loidl (1989), Zickler and Kleckner (1999) and many others, it is reaffirmed that meiosis is a complex, coordinated activity involving several genes and that mutation in any one of these leads to irregularities.…”

Section: Discussionsupporting

confidence: 80%

“…Such genes cause multipolar telophase I/II that result in additional number of microspores or formation of micronuclei (Sjödin 1970). Loidl (1989) described the reduction in the meiotic chromosome pairing and reduced number of chiasmata after exposing Allium ursinum to elevated temperatures and explained that these were due to i) failure of homologous recognition, ii) accumulation of additional material between pairing axes (which also affects completed SCs), and iii) aggregation of large numbers of non-homologous axes. Henderson (1988) in grasshopper Schistocerca observed heat increased chiasma frequency if administered at the spermatogonial stage and late zygotene/early pachytene stage.…”

Section: Discussionmentioning

confidence: 99%

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Ethyl Methane Sulphonate (EMS) Induced (Partial) Asynaptic Mutant in Faba Bean (Vicia faba L.)

Joshi

Verma

2005

CYTOLOGIA

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Summary One partial asynaptic mutant of Vicia faba (2nϭ12) was isolated from M 2 population of 0.2% EMS (Ethyl Methane Sulphonate) treated seeds. The mutant was designated as 'medium strong asynaptic mutant'. At diakinesis/metaphase I of meiosis number of univalents ranged from 2-8 in 90% of PMCs in M 2 and 2-4 in 44% of PMCs in M 3 . There was a significant decrease in the number of chiasmata in this plant as compared to the control. The metacentric chromosome pair did not show univalence. The pollen fertility was also reduced by 35%. The inheritance of this asynaptic mutant was probably of monogenic recessive type.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Ethyl Methane Sulphonate (EMS) Induced (Partial) Asynaptic Mutant in Faba Bean (Vicia faba L.)

Joshi

Verma

2005

CYTOLOGIA

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“…This trend may represent a regulatory connection between temperature sensing and CO control, or the mechanical process of CO may be sensitive to temperature. Studies in Locusta migratoria and Allium ursinum indicate that extreme temperatures can interfere with chromosome mechanics such as synapsis, leading to a decrease in CO frequency (19,20). Interestingly, temperature studies in Neurospora crassa reveal a temperature optima for COs (in one interval) at moderate temperatures (21).The fluorescent assay system described here will enable more detailed genetic analysis of the relationship between environmental conditions and meiotic recombination.…”

Section: Resultsmentioning

confidence: 96%

Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis

Francis

Lam²,

Harrison³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

180

197

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Recombination, in the form of cross-overs (COs) and gene conversion (GC), is a highly conserved feature of meiosis from fungi to mammals. Recombination helps ensure chromosome segregation and promotes allelic diversity. Lesions in the recombination machinery are often catastrophic for meiosis, resulting in sterility. We have developed a visual assay capable of detecting Cos and GCs and measuring CO interference in Arabidopsis thaliana. This flexible assay utilizes transgene constructs encoding pollen-expressed fluorescent proteins of three different colors in the qrt1 mutant background. By observing the segregation of the fluorescent alleles in 92,489 pollen tetrads, we demonstrate (i) a correlation between developmental position and CO frequency, (ii) a temperature dependence for CO frequency, (iii) the ability to detect meiotic GC events, and (iv) the ability to rapidly assess CO interference.cross-over ͉ meiosis ͉ tetrad ͉ gene conversion ͉ interference

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“…Silver staining was done according to Loidi and Jones (1986) and for the transfer of slides into electron microscopic preparations the schedule given by Loidl (1989) was followed. For evaluation individual configurations from enlarged EM micrographs were traced on transparent paper to facilitate identification, and measured using a digitizer.…”

Section: Methodsmentioning

confidence: 99%

EM analysis of meiotic chromosome pairing in a pentaploid Achillea hybrid

1990

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Presynaptic alignment occurs at zygotene and early pachytene in a pentaploid hybrid between Achillea collina (4x) and A. millefolium (6x). Mostly, the five corresponding chromosomes of each set are assorted into a group of three (two synapsed plus one aligned) and a group of two (synapsed); groups of five axes by alignment and/or pairing are extremely rare. This reflects different degrees of homology between the five genomes and allows inferences about the possible phylogeny and genome formulas of the tetraploid (AASS) and hexaploid (AASSTF) involved. The alignment as subgroups of three and two chromosomes in their 5x hybrid also indicates that synaptic alignment responds to slightly different degrees of homology and is decisive regarding which chromosomes will finally pair effectively and cross over. At early zygotene, alignment is weakly expressed, arguing against premeiotic associations as a precondition for homologous alignment and subsequent synaptonemal complex formation. At late pachytene, synapsis is maximized by autosynapsis of chromosomes which do not take part in the preceding homologous two-by-two synapsis.

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Effects of elevated temperature on meiotic chromosome synapsis in Allium ursinum

Cited by 61 publications

References 48 publications

Ethyl Methane Sulphonate (EMS) Induced (Partial) Asynaptic Mutant in Faba Bean (Vicia faba L.)

Ethyl Methane Sulphonate (EMS) Induced (Partial) Asynaptic Mutant in Faba Bean (Vicia faba L.)

Pollen tetrad-based visual assay for meiotic recombination in Arabidopsis

EM analysis of meiotic chromosome pairing in a pentaploid Achillea hybrid

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