Effects of electroacupuncture on rats with cognitive impairment: An iTRAQ-based proteomics analysis

Sa, Zhe-yan; Jinsen, Xu; Pan, Xiaohua; Zheng, Sujun; Huang, Qianru; Long, Wei; Zhu, Xiao-xiang; Lan, Cailian; Ye, Xiao-ran

doi:10.1016/j.joim.2022.11.001

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…Reducing neuroinflammation by inhibiting the TLR4 pathway may be an effective strategy for improving cognitive impairment after stroke. Some studies have found that EA at GV24 and GV20 can affect the proteomics changes of hippocampus in rats with cognitive impairment, and the up regulation of DAMPs such as heat-shock protein β1 (Hspb1) may participate in the molecular mechanism of EA to improve cognitive impairment ( Sa et al, 2023 ). EA at Taixi (KI3) and GV20 can reduce the expression of neuroinflammatory proteins in the hippocampus of gerbils that had undergone BCCAO, including ionized calcium binding junction molecule 1, TLR4, TNF-α and phospho-extracellular signal-regulated kinase (pERK) to improve inflammatory response and cognitive impairment ( Yang et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

The effects and mechanisms of acupuncture for post-stroke cognitive impairment: progress and prospects

Wang

Liu³

et al. 2023

Front. Neurosci.

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Stroke is one of the important causes of both disability and death worldwide, which is very common in older adults. Post-stroke cognitive impairment (PSCI) is a common secondary damage of stroke, which is the main cause of long-term disability and decreased quality of life in stroke patients, which brings a heavy burden to society and families. Acupuncture, as one of the oldest and widely used worldwide techniques in Chinese medicine, is recommended by the World Health Organization (WHO) as an alternative and complementary strategy for improving stroke care. This review comprehensively summarizes literature from the last 25 years, showing that acupuncture can exert strong beneficial effect on PSCI. The mechanisms of acupuncture on PSCI involves anti-neuronal apoptosis, promoting synaptic plasticity, alleviating central and peripheral inflammatory reactions, and regulating brain energy metabolism disorders (including improving cerebral blood flow, glucose utilization and mitochondrial structure and function, etc.), etc. The effects and mechanisms of acupuncture on PSCI reviewed in this study provides scientific and reliable evidence for acupuncture application for PSCI.

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Section: Resultsmentioning

confidence: 99%

The effects and mechanisms of acupuncture for post-stroke cognitive impairment: progress and prospects

Wang

Liu³

et al. 2023

Front. Neurosci.

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“…Research conducted by Yaru Liu and colleagues demonstrated that electroacupuncture may improve cognitive impairment caused by vascular dementia by inhibiting the JNK signaling pathway and reducing apoptosis in the hippocampus [ 30 ]. Additionally, through behavioral testing and proteomic analysis, Sa and colleagues found that electroacupuncture can improve the proteome in the hippocampal region of rats with cognitive impairment, which is associated with the upregulation of heat shock proteins in the brain via electroacupuncture treatment [ 31 ]. In this study, we also observed an improvement in hippocampal function in the brains of rats with PSCI through behavioral analysis, and found that the performance of rats treated with electroacupuncture was significantly improved compared with that of the untreated rats in the Water Maze test, and the difference between the number of traversing platforms in the late stage of training and that of the sham surgery group of rats was smaller.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

Bian,

Liu,

et al. 2024

NeuroReport

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This study aims to investigate how electroacupuncture regulates the learning and memory abilities of poststroke cognitive impairment (PSCI) rats through the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia. Thirty male rats were randomly divided into three groups: sham surgery group, PSCI model group, and electroacupuncture group, with 10 rats in each group. Middle cerebral artery occlusion was used to establish the PSCI model. The Zea Longa method was used to score the rats’ neurological function. Electroacupuncture was utilized for 21 days to improve PSCI. The learning and memory abilities of rats were tested using the Morris water maze. Hematoxylin–eosin staining and immunofluorescence were used to find the hippocampus’ pathological changes. The concentration of interleukin-1β, interleukin-6, tumor necrosis factor-α, and interleukin-18 were detected by ELISA. The mRNA expression levels of associated inflammatory corpuscles were measured by quantitative real-time PCR. The protein expression levels of TLR4, MyD88, NF-κB, and NLRP3 were measured using western blotting. Electroacupuncture improved not only the learning and memory abilities of PSCI rats but also hippocampal morphology. Electroacupuncture inhibited the activation of microglia and the TLR4/NF-κB/NLRP3 signaling pathway. Electroacupuncture also reduced proinflammatory factors and restrained the mRNA levels of NLRP3-associated inflammatory cytokines. Its mechanism was related to inhibiting the expression of the TLR4/NF-κB/NLRP3 signaling pathway, attenuating the release of inflammatory factors, and regulating the activation of hippocampal microglia in the brain.

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PCSK9 inhibitor effectively alleviated cognitive dysfunction in a type 2 diabetes mellitus rat model

Yang,

Wang,

Wang

et al. 2024

PeerJ

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Background The incidence of diabetes-associated cognitive dysfunction (DACD) is increasing; however, few clinical intervention measures are available for the prevention and treatment of this disease. Research has shown that proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors, particularly SBC-115076, have a protective effect against various neurodegenerative diseases. However, their role in DACD remains unknown. In this study, we aimed to explore the impact of PCSK9 inhibitors on DACD. Methods Male Sprague-Dawley (SD) rats were used to establish an animal model of type 2 diabetes mellitus (T2DM). The rats were randomly divided into three groups: the Control group (Control, healthy rats, n = 8), the Model group (Model, rats with T2DM, n = 8), and the PCSK9 inhibitor-treated group (Treat, T2DM rats treated with PCSK9 inhibitors, n = 8). To assess the spatial learning and memory of the rats in each group, the Morris water maze (MWM) test was conducted. Hematoxylin-eosin staining and Nissl staining procedures were performed to assess the structural characteristics and functional status of the neurons of rats from each group. Transmission electron microscopy was used to examine the morphology and structure of the hippocampal neurons. Determine serum PCSK9 and lipid metabolism indicators in each group of rats. Use qRT-PCR to detect the expression levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α) in the hippocampal tissues of each group of rats. Western blot was used to detect the expression of PCSK9 and low-density lipoprotein receptor (LDLR) in the hippocampal tissues of rats. In addition, a 4D label-free quantitative proteomics approach was used to analyse protein expression in rat hippocampal tissues. The expression of selected proteins in hippocampal tissues was verified by parallel reaction monitoring (PRM) and immunohistochemistry (IHC). Results The results showed that the PCSK9 inhibitor alleviated cognitive dysfunction in T2DM rats. PCSK9 inhibitors can reduce PCSK9, total cholesterol (TC), and low-density lipoprotein (LDL) levels in the serum of T2DM rats. Meanwhile, it was found that PCSK9 inhibitors can reduce the expression of PCSK9, IL-1β, IL-6, and TNF-α in the hippocampal tissues of T2DM rats, while increasing the expression of LDLR. Thirteen potential target proteins for the action of PCSK9 inhibitors on DACD rats were identified. PRM and IHC revealed that PCSK9 inhibitors effectively counteracted the downregulation of transthyretin in DACD rats. Conclusion This study uncovered the target proteins and specific mechanisms of PCSK9 inhibitors in DACD, providing an experimental basis for the clinical application of PCSK9 inhibitors for the potential treatment of DACD.

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Effects of electroacupuncture on rats with cognitive impairment: An iTRAQ-based proteomics analysis

Cited by 3 publications

References 31 publications

The effects and mechanisms of acupuncture for post-stroke cognitive impairment: progress and prospects

The effects and mechanisms of acupuncture for post-stroke cognitive impairment: progress and prospects

Electroacupuncture improves the learning and memory abilities of rats with PSCI by attenuating the TLR4/NF-κB/NLRP3 signaling pathway on the hippocampal microglia

PCSK9 inhibitor effectively alleviated cognitive dysfunction in a type 2 diabetes mellitus rat model

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