Effects of electrical stimulation on cell activity, cell cycle, cell apoptosis and β‑catenin pathway in the injured dorsal root ganglion cell

Abstract

The present study aimed to investigate the effects of electrical stimulation (eS) on cell activity, cell cycle and apoptosis in injured rat dorsal root ganglion (drG) cells induced by cyclic mechanical stretching (cMS). The present study also investigated whether the Wnt/β-catenin pathway is involved in this process. injury and eS models were established in drG cells. Then, cell activity was detected using a cell counting Kit-8 and 5-ethynyl-2'-deoxyuridine-594 cell proliferation assay kit. cell cycle distribution was detected using a cell cycle detection kit. apoptosis was detected using an annexin V-FiTc apoptosis detection kit, and Wnt/β-catenin pathway-associated proteins were detected using western blotting. The present study demonstrated that cMS decreased drG cell activity, increased the number of cells in the S phase, promoted cell apoptosis and inhibited the Wnt/β-catenin pathway. in addition, eS significantly increased the proliferation activity of drG cells, increased the number of cells in the G2 phase, decreased the apoptotic rate and activated the Wnt/β-catenin pathway, ultimately reversing the injury caused by cMS. Following inhibition of the Wnt/β-catenin signaling pathway using XaV939, the effects of eS were weakened. in conclusion, the present study demonstrated that eS may reverse cMS-induced injury in drG cells, and that the Wnt signaling pathway may be involved in this process.

Effect of Electrical Stimulation on Ocular Cells: A Means for Improving Ocular Tissue Engineering and Treatments of Eye Diseases

“…Hu et al have recently demonstrated that ES could significantly promote the proliferation activity of dorsal root ganglion cells (DRGs) and inhibited cell apoptosis. The results of this study also showed that ES could activate the Wnt/ β -catenin signaling pathway and exert a protective effect on the injured DRGs [ 43 ]. Recently, it has been reported that ES increases the expression of mRNA of brain-derived neurotrophic factor (BDNF) and consequently its protein level in the Müller cell in vitro [ 44 ].…”

Electrical Stimulation in Cartilage Tissue Engineering

“…To exclude possible cytotoxic effects, it is necessary to prove cell viability and proliferative activity after ES, which can be performed via fluorescent imaging (Live/Dead, Calcein AM, EdU) or metabolic assays (CCK-8, AlamarBlue etc.) [ 112 ]. The direct effect of electrical stimulation on the cell membrane should be monitored using VGCC inhibitors, while the creation of micropores can be proved via the Yo-Pro-1 uptake [ 113 ].…”

Application of Conductive Hydrogels on Spinal Cord Injury Repair: A Review

“…This can limit the effectiveness of the hydrogels in promoting axonal growth. Recent studies , reported that electrical fields may direct axonal growth by influencing various signaling pathways and cellular processes. This guidance can facilitate the axons to grow through the glial scars’ border or inhibit GFAP positive cell marker expression that reduces the glial cell proliferation at the injury site. , …”