Effects of egg yolk lecithin/milk fat globule membrane material ratio on the structure and stability of oil-in-water emulsions

Ultrasound modification on milk fat globule membrane and soy lecithin to improve the physicochemical properties, microstructure and stability of mimicking human milk fat emulsions

“…Dissolved MFGM and SL (1:1, w/w) in distilled water to prepare a solution at a concentration of 20 mg/mL [4] , [15] , and added sodium azide (0.02 %, w/w) to the solution to prevent microbial growth. The solution was stirred magnetically for 1 h (20 ± 2 °C), and the resulting solution was called MFGM-SL complex.…”

“…Zeta potential analyzer (Nano-Z, Malvern Instruments Ltd., Malvern, UK) and S3500 laser particle sizer (Microtrac Inc., Montgomery Ville, USA) were used to detect the particle size and zeta potential of different MFGM-SL complex and mimicking MFG emulsion with different ultrasonic pretreatment. Detection methods referred to previous report [4] . Briefly, dilute 0.1 mL MFGM-SL complex or mimicking MFG emulsion with ultrapure water to 50 mL to achieve 10 % shading.…”

“…Stability constants and encapsulation efficiency (EE) of mimicking MFG emulsion are detected based on the previous method [4] , [8] . Briefly, dispense the emulsion (5 mL) into dialysis bags and dialyze for 16 h in 0.005 mol/L PBS buffer (500 mL, pH 7.0).…”

“…Therefore, there has been increasing focus on the role of MFGM and their use in simulated human milk fat globule (MFG) emulsions in recent years. However, most of the MFGM materials used in these studies were MFGM-rich whey protein powders, which has a significantly lower content of phospholipids (PLs) (6 %–10 %) compared to PLs found in HM MFGM (60 %–65 %) [3] , [4] , [5] . Phospholipid (PL) has the functions of promoting fat metabolism, enhancing immunity, and improving brain and nervous system development [6] .…”

“…Chen et al [9] reported that adding lecithin or MFGM to whey protein isolate improves emulsion stability by increasing the surface coverage of PL. Yu et al [4] prepared the model emulsion by mixing PL with MFGM material to create the aqueous phase, and then combining the aqueous phase with the oil phase through high-pressure homogenization. This study also proposed that adding PL to MFGM could enhance the structure of MFG.…”