Effects of early dietary intervention with a fermentable fibre on colonic microbiota activity and mucin gene expression in newly weaned rats

Paturi, Gunaranjan; Butts, Christine A.; Stoklosinski, Halina; Ansell, Juliet

doi:10.1016/j.jff.2012.02.013

Cited by 41 publications

(30 citation statements)

References 47 publications

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“…Fresh faecal collections for bacterial analysis were made at the end of each balance period and before any subsequently consumed diet would have had time to reach the faeces. Selected bacterial species were quantified by real‐time polymerase chain reaction (RT‐PCR) as described previously (Paturi et al ., ). Genomic DNA of the bacteria in faecal contents was extracted by following the QIAamp DNA stool mini kit protocol (Qiagen, Melbourne, Vic., Australia).…”

Section: Methodsmentioning

confidence: 97%

Effects of kiwifruit and mixed dietary fibre on faecal properties and microbiota in rats: a dose–response analysis

Monro

Paturi

Mishra

2017

Int J of Food Sci Tech

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Summary In this study, a rat model was used to explore the interaction of kiwifruit with co‐consumed mixed dietary fibre. Rats were used in three consecutive trials in which faecal properties and composition, and bacterial populations were examined. In trial 1 diets, content of a dietary fibre mixture (DFM; Raftiline–citrus fibre–wheat fibre) was increased from 0% to 20%. In trial 2, dried kiwifruit pulp (KFP) content was increased from 0% to 20%. In trial 3, KFP was increased from 0% to 20% in a diet containing a 20% basal content of the DFM. The KFP caused a small dose‐dependent increase in faecal bulk and water‐holding capacity and had much less effect than the DFM. Faecal bacterial populations examined were stable across all diets and intakes of DFM and KFP. The disappearance of fermentable fibre during hind gut passage was not reduced with increasing KFP. Therefore, kiwifruit may not only have the beneficial effect of extending fermentation distally in the colon through gut activation, but may do so without disrupting the bacterial ecosystem and its functions.

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Section: Methodsmentioning

confidence: 97%

Effects of kiwifruit and mixed dietary fibre on faecal properties and microbiota in rats: a dose–response analysis

Monro

Paturi

Mishra

2017

Int J of Food Sci Tech

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“…An aliquot of cecal bacterial genomic DNA extracted from each sample (QIAamp DNA stool mini kit, Qiagen) was used for microbial quantification. Real‐time PCR quantification was performed as earlier described by Paturi and others (). Reference bacterial isolates used to generate standard curves for microbial quantification were: Bacteroides fragilis NZRM 964, Bifidobacterium adolescentis ATCC 15703, Butyrivibrio proteoclasticus B316, Clostridium perfringens ATCC 13124, Enterococcus faecalis AGR 991, Lactobacillus reuteri DPC 16, and Escherichia coli ATCC 35150.…”

Section: Methodsmentioning

confidence: 99%

Influence of Green and Gold Kiwifruit on Indices of Large Bowel Function in Healthy Rats

Paturi

Butts

Bentley‐Hewitt

et al. 2014

Journal of Food Science

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The effects of kiwifruit on large bowel health were investigated in healthy rats. Four-week old Sprague-Dawley rats were given diets containing 10% homogenized green kiwifruit, gold kiwifruit or 10% glucose solution (control) over 4 or 6 wk. Green kiwifruit increased the fecal output compared to control. Growth of certain bacterial species in cecum was influenced by both green and gold kiwifruit. A significant increase in cecal Lachnospiraceae in rats fed the green kiwifruit diet was observed at week 4. At week 6, green and gold kiwifruit diets assisted in improving colonic barrier function by upregulating the expression of mucin (MUC)-2, MUC3, Toll-like receptor (TLR)-4 or trefoil factor-3 genes. Gold kiwifruit consumption increased the colonic goblet cells per crypt at week 6. Significant negative correlations between E. coli and β-defensin 1 and TLR4 expression were observed. Consuming green and gold kiwifruit for 6 wk significantly altered the biomarkers of large bowel health; indicating that regularly consuming kiwifruit helps attain optimal digestive health.

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“…Therefore, guava byproduct may exert a major bulking action and increase faecal mass. Furthermore, the SDF and IDF can be partially fermented in the colon, altered microbiota composition and producing short chain fatty acids, these have been reported to exert hypolipidaemic and hypoglycaemic effects (Anderson et al, 2009;Paturi, Butts, Stoklosinski, & Ansell, 2012).…”

Section: Characterization Of Nutraceutical Compounds Of Juice By-prodmentioning

confidence: 99%

Juice by-products as a source of dietary fibre and antioxidants and their effect on hepatic steatosis

Amaya-Cruz

Rodríguez-González

Pérez‐Ramírez

et al. 2015

Journal of Functional Foods

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Effects of early dietary intervention with a fermentable fibre on colonic microbiota activity and mucin gene expression in newly weaned rats

Cited by 41 publications

References 47 publications

Effects of kiwifruit and mixed dietary fibre on faecal properties and microbiota in rats: a dose–response analysis

Effects of kiwifruit and mixed dietary fibre on faecal properties and microbiota in rats: a dose–response analysis

Influence of Green and Gold Kiwifruit on Indices of Large Bowel Function in Healthy Rats

Juice by-products as a source of dietary fibre and antioxidants and their effect on hepatic steatosis

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