Effects of duct ligation and orchidectomy on histochemical reactions in the hamster epididymis

Moniem, K. A.; Glover, T. D.; Lubicz-Nawrocki, C. W.

doi:10.1530/jrf.0.0540173

Cited by 31 publications

(19 citation statements)

References 6 publications

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“…Only the head/body region of the epididymis showed evidence of stimulation after arousal: weights of this region, unlike the tail, were significantly increased after arousal and this increase was partly inhibited by food restriction. This discrepancy of response between different regions of the bat epididymis appears to be similar to that in laboratory rodents in which the initial segments of the epididymis are apparently more dependent upon high levels of intraluminal testosterone than on plasma testosterone for stimulation and maintenance (Moniem, Glover & Lubicz-Nawrocki, 1978;Fawcett & Hoffer, 1979). The low level of peripheral androgen activity as indicated by the unstimulated state of the accessory glands and cauda epididymidis, in spite of the elevated plasma testosterone concentrations, suggests either that SBP may be exerting an inhibitory influence on peripheral androgen action, or that these tissues are relatively insensitive at this stage in their reproductive development to circulating androgens.…”

Section: Discussionsupporting

confidence: 56%

Relationship of food intake to the induction of plasma sex steroid-binding protein and testicular activity in immature male little brown bats (Myotis lucifugus lucifugus)

Damassa¹,

Gustafson²

1985

Reproduction

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Immature male little brown bats were aroused prematurely from their first hibernation and fed ad libitum (Group AL) or given a restricted diet (Group FR). All animals were weighed daily and the food intake of Group FR males was restricted to maintain their body weights at or near initial post-arousal values (5.3-6.8 g). The average body weights of Group AL males increased during the first week after arousal and then stabilized at a level which was 20% higher than those of Group FR males. The post-arousal induction of plasma SBP was similar in Groups FR and AL: plasma SBP activity was significantly increased 1 week after arousal and by 3 weeks had reached levels which were more than 10-fold higher than those of immature hibernating males which served as controls. Although food restriction had no effect on plasma SBP levels, it did inhibit reproductive development. Arousal-induced increases in testicular and epididymal (head/body) weights in Group FR males were less than 50% of those in Group AL males. However, histological examination of the testes revealed similar degrees of spermatogenic activation in both groups. Plasma testosterone concentrations were increased markedly in Groups FR and AL; values were generally lower in Group FR but wide individual variations were observed. Despite these elevated peripheral testosterone values, the accessory sex glands in both groups remained unstimulated.

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Section: Discussionsupporting

confidence: 56%

Relationship of food intake to the induction of plasma sex steroid-binding protein and testicular activity in immature male little brown bats (Myotis lucifugus lucifugus)

Damassa¹,

Gustafson²

1985

Reproduction

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“…The decrease in general and specific protein synthesis in regions 2-5 after ligation of the ductuli efferentes favours the hypothesis that testicular fluid is important in maintaining the functional activity of the caput epididymidis. This is commensurate with our previous work on the rat (Jones et al, 1980a,b) and the morphological findings of Danzo et al (1977), Moniem et al (1978) and Fawcett & Hoffer (1979), who showed that the presence of testicular fluid was necessary to prevent involution of the epithelium in the initial segment. Testicular fluid contains a wide range of different proteins (Koskimies & Kormano, 1975), one of which, androgen-binding protein, transports large amounts of testosterone into the epididymis.…”

Section: Discussionsupporting

confidence: 92%

Characterization of hormonally regulated secretory proteins from the caput epididymidis of the rabbit

Jones¹,

Glos²,

Brown³

1981

Biochemical Journal

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1. The incorporation of [35S]methionine into protein was investigated in tissue minces from different regions of the rabbit epididymis incubated in vitro. Rates of synthesis were in the order: epididymal regions 2-5 greater than region 7 greater than region 6 greater than region 1 greater than region 8 greater than ductus deferens greater than ductuli efferentes. 2. Separation of labelled proteins on polyacrylamide gels containing sodium dodecyl sulphate followed by fluorography revealed that one protein (mol.wt. approx. 90 000) was characteristic of region 1, four proteins (one of mol.wt. 54 000 and three of mol.wt. 20 000) were synthesized principally in regions 2-5, and one protein (mol.wt. 22 500) was produced mainly in regions 6, 7 and 8. 3. Castration for 14 days decreased incorporation of [35S]methionine into total protein to less than 10% of that in controls in all regions of the epididymis. However, testosterone treatment for a further period of 14 days restored protein synthesis to normal values in regions 6, 7 and 8, but not in region 1 or regions 2-5. In regions 2-5 the synthesis of three proteins of mol.wt. 20 000 declined after castration, but was not stimulated by exogenous testosterone. Since the 20 000-mol.wt. proteins were major tissue proteins, accounting for 16-25% of the total synthesized, they were used as markers for investigating hormone action in the epididymis. 4. Castration followed immediately by testosterone treatment or ligation of the ductuli efferentes resulted in a decrease in their synthesis, suggesting that they are partially dependent on factors in testicular fluid. Purification and characterization showed them to be acidic glycoproteins with a number of biochemical and immunological properties in common. 5. It is suggested that there is a synergistic action between blood androgens and factors in testicular fluid in regulating protein synthesis in the proximal regions of the rabbit epididymis.

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“…Similarly in the hamster, histological changes are first detected in the initial segment 1 day after castration, and with longer periods of time, changes are observed in the more distal portions of the epididymal tubule (Moniem, Glover & Lubicz-Nawrocki, 1978). Correlating with these morphological studies, large populations of spermatozoa with normal motility are still found in the distal cauda epididymidis 4 days after surgery (Foldesy & Bedford, 1981).…”

Section: Resultsmentioning

confidence: 76%

Effects of Short-Term Bilateral and Unilateral Castration and Androgen Replacement on the Metabolism of [3h]testosterone in Vitro by the Epididymis of the Immature Rat

Foldesy¹,

Leathem²

1981

Journal of Endocrinology

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The in-vitro metabolism of [3H]testosterone by the epididymis of the pubertal rat (55 days of age) has been examined after short-term bilateral and unilateral castration and androgen replacement. Bilateral castration did not decrease the metabolism of [3H]testosterone, but did result in a decline in the proportion of 5\g=a\-dihydrotestosterone produced and an increase in that of 5\g=a\-androstane-3\g=a\,17\g=b\-diol, androsterone and 5\g=a\-androstane-3,17-dione.Changes in metabolism occurred in the caput within 2 days after surgery, but not until 5 days after surgery in the cauda epididymidis. Daily testosterone treatment, which maintained prostatic growth in bilaterally castrated animals, did not restore normal androgen metabolism and increased further the production of androsterone and 5\g=a\-androstanedione by the cauda. In unilaterally castrated animals, androgen metabolism in epididymal tissue from the operated side was normal in the cauda but was indistinguishable in the caput from that of bilaterally castrated rats. These results indicate that (a) androgen metabolism by the caput, compared to that by the cauda, responds more quickly to androgen withdrawal and (b) that in the short term, normal androgen metabolism by the caput, but not the cauda, is dependent upon the presence of the ipsilateral testis. Furthermore, testosterone alone proved an inadequate replacement for bilateral castration which implies that the pubertal rat testis secretes additional compounds which are essential for normal function of the epididymis.

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Effects of duct ligation and orchidectomy on histochemical reactions in the hamster epididymis

Cited by 31 publications

References 6 publications

Relationship of food intake to the induction of plasma sex steroid-binding protein and testicular activity in immature male little brown bats (Myotis lucifugus lucifugus)

Relationship of food intake to the induction of plasma sex steroid-binding protein and testicular activity in immature male little brown bats (Myotis lucifugus lucifugus)

Characterization of hormonally regulated secretory proteins from the caput epididymidis of the rabbit

Effects of Short-Term Bilateral and Unilateral Castration and Androgen Replacement on the Metabolism of [3h]testosterone in Vitro by the Epididymis of the Immature Rat

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