Effects of drought stress on the antioxidant system, osmolytes and secondary metabolites of Saposhnikovia divaricata seedlings

Physiological and antioxidative responses associated with drought tolerance of Lasiurus sindicus Henr. endemic to Thar desert, India

“…Under drought, accumulation of osmolytes like proline and sugars can maintain cell turgor through osmotic adjustments, protect proteins, organelles and membrane from oxidative damage (Krasensky and Jonak 2012). Proline accumulation under water deficit is a good marker of stress tolerance through osmoregulation and ROS scavenging in various stressed plants (Liu et al 2013;Men et al 2018). In the present study, increase in proline content indicates response of plants toward PEG-induced drought.…”

“…Drought stress-induced exacerbated ROS generation is well-recognized at cellular level, and increased antioxidant system regulates ROS homeostasis at both the production and consumption levels in plants. Furthermore, droughtinduced overproduction of ROS leads to increase the concentration of MDA through lipid peroxidation (Men et al 2018). Hence, the MDA is suitable marker for membrane lipid peroxidation and its higher level in water-stressed plants is related to excess of ROS (Huang et al 2013;Khan et al 2019).…”

“…(Gadi and Laxmi 2012) and Saposhnikovia divaricata (Turcz.) Schischk (Men et al 2018). Thus, high level of sugars and proline may protect L. sindicus seedlings mainly through osmoregulation and ROS homeostasis.…”

“…Roles of antioxidants like SOD, POD and CAT have been well documented in plants under drought (Devi et al 2012;Khan et al 2019). Some non-enzymatic antioxidants like ascorbate, glutathione and phenolic compounds synthesis are triggered in response to stress (Men et al 2018;Khan et al 2019). However, the activation and downregulation of antioxidative enzymes depending on plant genotypes, duration and stress intensity imposed to plants (Mickky and Aldesuquy 2017;Piwowarczyk et al 2017;Zhang et al 2020).…”

Comparative Transcriptomic and Physiological Analyses of Medicago sativa L. Indicates that Multiple Regulatory Networks Are Activated during Continuous ABA Treatment

“…For the roots tips, lipid peroxidation via MDA; the production of ROS, such as H 2 O 2 ; the activity of antioxidative enzymes, such as POD and CAT; the content of non-enzymatic antioxidants, such as GSH; and osmotic-adjusting substances, such as PRO, were determined using Comin Biochemical Test Kits (MDA-2-Y, H 2 O 2 -2-Y, POD-2-Y, CAT-2-Y, GSH-2-W and PRO-2-Y, respectively; Cominbio, Suzhou, China) in accordance with the manufacturer’s instructions. In brief, the MDA content was assayed based on the thiobarbituric acid-reactive substance assay according to the method of Castrejón and Yatsimirsky (1997) [73]; the H 2 O 2 content was determined by monitoring the absorbance of titanium-peroxide complex at 415 nm according to the method described by Men et al (2018) [74]; the POD activity was assayed based on the detection of the absorbance of the product at 470 nm in the reaction system according to the method of Toivonen and Sweeney (1998) [75]; the CAT activity was determined by measuring the rate of decomposition of H 2 O 2 (ε = 39.4 mM −1 cm −1 ) at 240 nm as described by Men et al (2018) [74]; the GSH content was measured by detecting the absorbance of 5,5′-Dithiobis-(2-nitrobenzoic acid)-GSH complex at 412 nm according to the method of Men et al (2018) [74] ; and the PRO content was measured using the acid ninhydrin method described by Vieira et al (2010) [76].…”