Effects of double-layer polarization and counterion condensation on the electrophoresis of polyelectrolytes

Yeh, Li‐Hsien; Hsu, Jyh‐Ping

doi:10.1039/c0sm00600a

Cited by 67 publications

(163 citation statements)

References 80 publications

(160 reference statements)

Supporting

Mentioning

155

Contrasting

Order By: Relevance

“…Accordingly, the scaled value of the fixed charge density of the polymer layer Q f ix is varied up to 50. The softness parameter λ −1 s for biological cells ranges from 0.1 to 10 nm [3,31,32]. In our present computation, we have considered the non-dimensional parameter β = λ s b ≤ 100.…”

Section: Resultsmentioning

confidence: 99%

Effect of core charge density on the electrophoresis of a soft particle coated with polyelectrolyte layer

2016

View full text Add to dashboard Cite

The electrophoresis of a charged soft particle with charged rigid core is considered under a weak imposed field condition. The rigid core of the soft particle is considered to have a finite dielectric permittivity and a fixed volume charge density. The electric potential distribution is determined by solving the Poisson-Boltzman equation out side the rigid core and a Poisson equation within the core along with continuity conditions on the core-shell interface. We have extended the analytic expression of Ohshima (Electrophoresis 27:526-533, 2006) for the electrophoretic mobility of a soft particle with a charged shell to include the effect of the volume charge density of the rigid core. Mobility based on the present expression matches exactly with the existing analytical solutions for a soft particle with an uncharged core. We have also made a comparison of our solution for mobility with an uncharged rigid core with the existing experimental results. The impact of the core charge density on the soft particle mobility is analyzed.

show abstract

Section: Resultsmentioning

confidence: 99%

Effect of core charge density on the electrophoresis of a soft particle coated with polyelectrolyte layer

2016

View full text Add to dashboard Cite

show abstract

“…Slip boundary condition is applied on the lateral boundaries of the two reservoirs, which are far away from the nanopore. A no‐slip boundary condition is imposed on the surface of the translating nanoparticle:

u (r, z) = u_{p} {bolde}_{z},

where u p is the translational velocity of DNA nanoparticle to be determined by requiring that the z ‐component net force acting on the particle to vanish :

F_{E} + F_{D} = 0 .

…”

Section: Mathematical Modelmentioning

confidence: 99%

Slowing down DNA translocation through a nanopore by lowering fluid temperature

et al. 2012

Self Cite

View full text Add to dashboard Cite

In the next-generation nanopore-based DNA sequencing technique, the DNA nanoparticles are electrophoretically driven through a nanopore by an external electric field, and the ionic current through the nanopore is simultaneously altered and recorded during the DNA translocation process. The change in the ionic current through the nanopore as the DNA molecule passes through the nanopore represents a direct reading of the DNA sequence. Due to the large mismatch of the cross-sectional areas of the nanopore and the microfluidic reservoirs, the electric field inside the nanopore is significantly higher than that in the fluid reservoirs. This results in high-speed DNA translocation through the nanopore and consequently low read-out accuracy on the DNA sequences. Slowing down DNA translocation through the nanopore thus is one of the challenges in the nanopore-based DNA sequencing technique. Slowing down DNA translocation by lowering the fluid temperature is theoretically investigated for the first time using a continuum model, composed of the coupled Poisson-Nernst-Planck equations for the ionic mass transport and the Navier-Stokes equations for the hydrodynamic field. The results qualitatively agree with the existing experimental results. Lowering the fluid temperature from 25 to 0°C reduces the translocation speed by a magnitude of about 6.21 to 2.50 mm/sK (i.e. 49.82 to 49.71%) for the salt concentration at 200 and 2000 mM, respectively, improving the read-out accuracy considerably. As the fluid temperature decreases, the magnitude of the ionic current signal decreases (increases) when the salt concentration is high (sufficiently low).

show abstract

“…Other theoretical investigations on the charged porous spheres can be found in the literature as well [40][41][42][43]. Among them, Keh et al [40] studied the suspensions of lowly charged porous sphere with an analytical approach.…”

Section: Introductionmentioning

confidence: 98%

“…He et al [41], on the other hand, studied suspensions of highly charged porous spheres and pointed out the crucial impact of polarization effect upon particle mobility. Yeh and Hsu [42] studied the motion of a charged porous sphere in a narrow cylindrical pore. They indicated that the double layer polarization and counterion condensation affect the particle motion.…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis of a single charged porous sphere in an infinite medium of electrolyte solution

Hsu

Lee

2013

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Effects of double-layer polarization and counterion condensation on the electrophoresis of polyelectrolytes

Cited by 67 publications

References 80 publications

Effect of core charge density on the electrophoresis of a soft particle coated with polyelectrolyte layer

Effect of core charge density on the electrophoresis of a soft particle coated with polyelectrolyte layer

Slowing down DNA translocation through a nanopore by lowering fluid temperature

Electrophoresis of a single charged porous sphere in an infinite medium of electrolyte solution

Contact Info

Product

Resources

About