Effects of donor characteristics and platelet in vitro time and temperature on platelet aggregometry

Silver, William P.; Keller, Michael P.; Teel, Rosemary; Silver, Donald

doi:10.1016/0741-5214(93)90117-5

Cited by 17 publications

(8 citation statements)

References 14 publications

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“…In spite of the statistical expectation of a large number of false positive tests, the frequency of specimens with minimal aggregation and absent release among healthy subjects is concerning. Females have been observed to have greater aggregation than males in some studies (Johnson et al, 1975;Meade et al, 1985;Silver et al, 1993;Becker et al, 2006) but not in others (Beyan et al, 2006;Seyfert et al, 2007;Rubak et al, 2012). Here, only epinephrine showed a gender difference.…”

Section: Discussioncontrasting

confidence: 48%

“…These data suggest that a morning fast prior to blood draw is not sufficient to eliminate the effect of inhibitory foods; an abstention from specific foods of 24 h may be required to eliminate their effect. Other studies have shown no effect of eating breakfast (Stegnar et al , ) or lunch (Silver et al , ) prior to LTA, but neither considered the intake of specific foods. Our study was limited by the large number of specimens excluded due to drug exposure and the complexity of the dietary information collected.…”

Section: Discussionmentioning

confidence: 95%

“…Females have been observed to have greater aggregation than males in some studies (Johnson et al , ; Meade et al , ; Silver et al , ; Becker et al , ) but not in others (Beyan et al , ; Seyfert et al , ; Rubak et al , ). Here, only epinephrine showed a gender difference.…”

Section: Discussionmentioning

confidence: 97%

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Gender, race and diet affect platelet function tests in normal subjects, contributing to a high rate of abnormal results

et al. 2014

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Summary To assess sources of variability in platelet function tests in normal subjects, 64 healthy young adults were tested on 2–6 occasions at 2 week intervals using 4 methods: platelet aggregation (AGG) in platelet-rich plasma (PRP) in the Bio/Data PAP-4 Aggregometer (BD) and Chrono-Log Lumi-Aggregometer (CL); and AGG in whole blood (WB) in the CL and Multiplate Platelet Function Analyzer (MP), with ATP release (REL) in CL-PRP and CL-WB. Food and medication exposures were recorded prospectively for 2 weeks prior to each blood draw. At least one AGG abnormality was seen in 21% of 81 drug-free specimens with CL-PRP, 15% with CL-WB, 13% with BD-PRP, and 6% with MP-WB, increasing with inclusion of REL to 28% for CL-PRP and 30% for CL-WB. Epinephrine AGG and REL were significantly reduced in males (P<0.0001). Ristocetin AGG and collagen and thrombin REL were significantly reduced in Blacks (P<0.0001). One-third of specimens drawn following flavonoid-rich food exposures had aberrant results, compared to 8.5% of specimens without such exposures (P=0.0035). PRP tests had less intra-individual variation than WB tests. Gender, race, diet, and test system affected results of platelet function testing in healthy subjects, suggesting caution when interpreting the results of platelet function testing in patients.

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Section: Discussioncontrasting

confidence: 48%

Section: Discussionmentioning

confidence: 95%

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Gender, race and diet affect platelet function tests in normal subjects, contributing to a high rate of abnormal results

et al. 2014

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“…The blood from 8 donors was tested at six temperatures (15,20,24,28,32, and 37°C). Due to the time required for CPF analysis, only three temperatures could be tested simultaneously, therefore three 60-cc syringes of blood were drawn from the same donor for a given experiment, and each syringe was exposed to a dierent temperature (all three simultaneously), as summarized in Table I.…”

Section: Experimental Design To Examine the Effect Of Temperaturementioning

confidence: 99%

Hypothermia‐induced platelet aggregation in heparinized flowing human blood: Identification of a high responder subpopulation

Hall¹,

Goodman²,

Alston³

et al. 2001

American J Hematol

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Cold-induced platelet aggregation (CIPA) in PRP has previously been documented in connection with platelet preservation (4±15°C). This report describes hypothermia-induced platelet aggregation (HIPA) in whole blood and at temperatures used in open-heart surgery (24±32°C). HIPA (speci®cally, the formation of occlusive aggregates) was studied in human whole blood. Fresh heparinized (1.5 U/ml) human blood was cooled and maintained at target temperatures (15, 20, 24, 28, 32, or 37°C) as it¯owed (1 ml/min) through 75-cm long 1/32" internal diameter polymer conduit. The formation of aggregates in the tubing was veri®ed using optical video microscopy and was quanti®ed by a lightscattering method and a constant-pressure ®ltration method. Donors were tested at least twice at each target temperature and were classi®ed into three separate response regimes (Low, Medium, and High) on the basis of the number of aggregates and the duration of their appearance. The screening of 121 donors (average age 22.3 4.3 years) for HIPA at 24°C (the temperature of maximum response) indicated 14% High Responders, 18% Medium Responders, and 68% Low Responders. HIPA was inhibited by EDTA, citrate, PGE 1 , and Tiro®ban, but not by aspirin, and it was enhanced by elevated heparin levels. HIPA was consistently noted in the blood of a subpopulation of donors, and the associated platelet aggregates in the blood of High Responders were rigid and occlusive. It is postulated that such aggregates may contribute to cognitive dysfunction noted in patients undergoing hypothermic open-heart surgery, and that postulus is being investigated. Am.

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“…It acts by chelating extracellular Ca 2+ , leading to a decrease in Ca 2+ levels in the sample from about 1.2 to 0.1 mmol/l [4][5][6]. Due to the fact that platelets are dependent on Ca 2+ for normal function, the platelet viability is markedly reduced in citrated blood which is a critical point with respect to the transportation time of samples to the coagulation laboratory [7]. In many laboratories it is the rule that the investigation of aggregometry in PRP and in WB should start 15-30 min after blood collection and must be completed within 3-4 h [2,3,8,9].…”

Section: Introductionmentioning

confidence: 99%

Investigations of Platelet Function in Whole Blood with BAPA as Anticoagulant

Tauer

Lohse²,

König³

et al. 2010

Transfus Med Hemother

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Background: Citrate anticoagulation of blood results in non-physiologically low calcium concentration and rapid deterioration of platelet viability. Benzylsulfonyl-D-ArgPro-4-amidinobenzylamide (BAPA) as anticoagulant maintains the physiological calcium level and seems to retain platelet function (PF) over a time period of at least 24 h. We evaluated PF in BAPA-anticoagulated peripheral whole blood (WB) between 0.5 and 30 h after blood collection. Methods: In WB from 21 healthy volunteers (15 women, 6 men, age range 19–57 years) platelet aggregation (PA) was determined by impedance method and ATP release by luminometry. Platelet response was tested by ADP (10 and 20 mmol/l) and collagen (1 and 2 mg/ml) between 0.5 and 30 h after blood collection. Results: Parameters of ADP-induced PA showed stable values until 6.5 h after blood collection followed by a significant decline. PA in response to collagen was stable up to 25 h of storage. ATP release induced by collagen displayed a continuous, significant decrease over time. Conclusion: Preservation of platelet response in BAPAanticoagulated blood depends on the applied agonist showing that collagen-induced PA is more stable compared to ADP known as a weak agonist in WB.

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Effects of donor characteristics and platelet in vitro time and temperature on platelet aggregometry

Abstract: It is recommended that platelet-rich plasma for platelet aggregation testing be maintained at room temperature and be used between 2 and 4 hours after platelet donation.

Cited by 17 publications

References 14 publications

Gender, race and diet affect platelet function tests in normal subjects, contributing to a high rate of abnormal results

Gender, race and diet affect platelet function tests in normal subjects, contributing to a high rate of abnormal results

Hypothermia‐induced platelet aggregation in heparinized flowing human blood: Identification of a high responder subpopulation

Investigations of Platelet Function in Whole Blood with BAPA as Anticoagulant

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