Effects of donor and acceptor's fluorescence lifetimes on the method of applying Förster resonance energy transfer in STED microscopy

Deng, Suhui; Chen, Jianfang; Gao, Zhen; Chen, Fan; Yan, Qiurong; Wang, Yuhao

doi:10.1111/jmi.12608

Cited by 10 publications

(6 citation statements)

References 31 publications

(45 reference statements)

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“…Combination with other readouts (such as force, electrophysiology or mass spectrometry) enhances the information content of imaging experiments, and it will be interesting to develop such hybrid approaches to be more accessible for biology-driven applications. Furthermore, combining SRM with fluorescence spectroscopy techniques such as fluorescence recovery after photobleaching (FRAP) 130,131 , Förster resonance energy transfer (FRET) 132,133 , and FCS 134 will further expand its applications to the study of structural dynamics and molecular interactions in living cells.…”

Section: Srm As a Tool To Inform Biologymentioning

confidence: 99%

Super-resolution microscopy demystified

et al. 2019

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Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

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Section: Srm As a Tool To Inform Biologymentioning

confidence: 99%

Super-resolution microscopy demystified

et al. 2019

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“…Later, this principle was successfully applied for improving the resolution of STED microscopy 2-3-fold. In this approach, named FRET-assisted STED (FASTED) excited acceptors are depleted in the outer rim of the excitation spot leading to donor de-excitation and improved spatial resolution for the aforementioned principles [155,156].…”

Section: Don't Dos In Fretmentioning

confidence: 99%

Quo vadis FRET? Förster’s method in the era of superresolution

Szabó

Szendi-Szatmári

Szöllősi

et al. 2020

Methods Appl. Fluoresc.

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Although the theoretical foundations of Förster resonance energy transfer (FRET) were laid in the 1940s as part of the quantum physical revolution of the 20th century, it was only in the 1970s that it made its way to biology as a result of the availability of suitable measuring and labeling technologies. Thanks to its ease of application, FRET became widely used for studying molecular associations on the nanometer scale. The development of superresolution techniques at the turn of the millennium promised an unprecedented insight into the structure and function of molecular complexes. Without downplaying the significance of superresolution microscopies this review expresses our view that FRET is still a legitimate tool in the armamentarium of biologists for studying molecular associations since it offers distinct advantages and overcomes certain limitations of superresolution approaches.

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“…Recent technological developments using dark acceptor FRET couples (consisting of a fluorescent Donor and an absorbing but non-emitting or "dark" Acceptor) and fast FLIM imaging allow such multiplexing and an intensity-independent read-out of FRET efficiency [10]. Furthermore, super-resolution optical microscopy such as STED takes benefit of such probes by a resolution increase to nm scale due to non-linear effects [11]. So far, however, no reported applications in the field of platelet research can be found.…”

Section: Labeled Platelets Isolated From Transgenic Mice Expressing a Fluorescent Markermentioning

confidence: 99%