Effects of DL-Methionine at various doses per se on liver in albino rats: An experimental study

Abstract

Objective: Oxidative stress and diminished glutathione play critical roles in the pathogenesis of liver disease. Synthesis of glutathione, the most abundant mammalian antioxidant, is regulated at the substrate level by cysteine, which is synthesized from homocysteine via the trans-sulfuration pathway. Hence, the research study was aimed to explore the effects of single oral dose per se DL-Methionine.: Albino rats were separated into Material and Methods three groups for the experimental study. After overnight fasting, the albino rats belonging to Group I Control Group were administered Distilled Water 10 ml/kg orally; Group II Met. 700 mg/kg & Group III Met. 1400 mg/kg were administered DL-Methionine as a single oral dose by intragastric cannula. After 24-hours of post-treatment, serum level was evaluated to demonstrate the per se effects of DL-Methionine on liver. Also, the liver samples from each group were examined for the histopathological study.: The histopathological findings of DL-Methionine per se Results treated rats were compared with the control group. Gross examination revealed near to normal appearance & morphologic changes of liver tissue showed normal morphology and normal hepatic parenchymal cells. We observed that DL-Methionine as a single oral dose of 700 mg/kg and 1400 mg/ kg although caused alteration in levels of liver enzymes the alteration was not statistically significant.: DL-Methionine per se although showed alteration Conclusion in the biochemical parameters, they were not found to be significant so as to be considered as hepatotoxic agent. Also, Methionine is commonly used nutritional, essential amino acid, is preferred in the treatment of Paracetamol poisoning.