Effects of divalent cations and functionally related substances on the yeast to mycelium transition in<i>Sporothrix schenckii</i>

Alsina, A.; Valle, Nuri Rodrı́guez-del

doi:10.1080/00362178485380031

Cited by 23 publications

(8 citation statements)

References 11 publications

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“…Magnesium and zinc have also been reported to influence a yeast-mycelium transition in other dimorphic fungi. Alsina & Rodriguez-Del Valle (1984) suggested an inhibitory role for zinc and magnesium in the regulation of the yeast-mycelium transition in Sporothrix schenkii. Inhi bition of mycelium development by Zn2+ has also been reported in C. albicans (Sol1 et al, 1981; Sabie & Gadd, 1990) and Histoplasma capsulatum (Pine & Peacock, 1958), while a requirement for Mg2+ has been demonstrated for germ tube production in C. albicans (Walker et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Calcium involvement in dimorphism of Ophiostoma ulmi, the Dutch elm disease fungus, and characterization of calcium uptake by yeast cells and germ tubes

Gadd

Brunton

1992

Journal of General Microbiology

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Section: Discussionmentioning

confidence: 99%

Calcium involvement in dimorphism of Ophiostoma ulmi, the Dutch elm disease fungus, and characterization of calcium uptake by yeast cells and germ tubes

Gadd

Brunton

1992

Journal of General Microbiology

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“…The responses have been either stimulation of mycelial growth as with Coccidioides irnmitis (Roessler et al, 1946) or suppression of the mycelial phase, e.g. Histoplasma capsulatum (Pine & Peacock, 1958), Mucor rouxii (Bartnicki-Garcia & Nickerson, 1962), Sporothrix schenckii (Alsina & Rodriguez-Del Valle, 1984) and C. albicans (Widra, 1964;Yamaguchi, 1975;Bedell & Soll, 1979;Soll et al, 1981 ;Sabie & Gadd, 1990). These data suggest that zinc may have an important role in the yeast-mycelium dimorphism of A .…”

Section: Varying the Iriput Concentration Of Zn2+mentioning

confidence: 94%

Influence of Zn2+ on yeast-mycelium dimorphism and exopolysaccharide production by the fungus Aureobasidium pullulans grown in a defined medium in continuous culture

Reeslev

Jørgensen²,

Jørgensen

1993

Journal of General Microbiology

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The yeast-mycelium dimorphism of Aureobasidium pullulans was studied in continuous culture in a defined medium. At a constant dilution rate (0-08 h-') the morphological status of the culture could be controlled by the input concentration of Zn2+. As the input concentration of Zn2+ was increased (in intervals from 0 to 7.6 p~) the culture shifted from a zinc-limited to a carbon-limited state. In this interval the culture gradually passed through three growth regimes based on morphology and concentration of exopolysaccharide and biomass. The first growth regime was found when the input concentration of Zn2+ was kept below 0.45 PM. Growth in this regime was zinclimited and more than 90% of the biomass was in the yeast growth form. An increase in the input concentration of Zn2+ in this growth regime led to a proportional increase in both the biomass and the concentration of exopolysaccharide. When the input concentration of Zn2+ was varied between 0.45 VM and 0.80 ~L M a second growth regime could be detected where simultaneous limitations in two nutrients were recognized. Although the carbon source (glucose) was exhausted an increase in the input concentration of Zn2+ led to a proportional increase in the steady-state biomass concentration. The increase in biomass concentration was at the expense of exopolysaccharide production, which gradually decreased. The culture, still being primarily limited by Zn2+, remained in the yeast growth form. In a third growth regime (input concentration of Zn2+ above 0430 p~) no increase in the steady-state biomass was seen when the input concentration of Zn" was increased. The concentration of residual glucose and exopolysaccharide was close to zero, and no further carbon could be diverted to an increase in the biomass. Glucose was the primary limiting nutrient. Increasing the input concentration of Zn" in this growth regime led to a gradual increase in the mycelial growth form at the expense of the yeast growth form. More than 90% of the biomass was in the mycelial growth form when an input concentration of Zn2+ of 7.6 PM was used.

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“…Sporothrix schenckii is a pathogenic dimorphic fungus that grows in the yeast or mycelium form, depending on the culture conditions [ 1,3 ]. These dimorphic transitions are reversible and offer a suitable model for the study of differentiation in a lower eukaryote.…”

Section: Introductionmentioning

confidence: 99%

“…Although the mechanism by which the yeast to mycelium transition is triggered is still unknown, the fact that the addition of calcium ions to the medium stimulated this transition [ 1] prompted us to examine the movement of this cation into and out of the cells during the first 6 h after the induction of germ tube formation. The effects of ionophore A23187 [18], compound R24571 [8] and cobalt ions [ 10] on germ tube formation and calcium fluxes were also tested.…”

Section: Introductionmentioning

confidence: 99%

Calcium uptake and efflux during the yeast to mycelium transition inSporothrix schenckii

Serrano

Valle

1990

Mycopathologia

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A study was made of calcium metabolism during germ tube formation in Sporothrix schenckii yeast cells. A net efflux of calcium was observed very early in the transformation process and remained constant thereafter. The efflux of calcium in yeast cells induced to form germ tubes was twice that observed in yeast cells not induced to form germ tubes. Two peaks of calcium uptake were observed in germ tube forming yeast cells at 30 and 300 minutes following inoculation, while non-induced yeast cells, a continuous increase in uptake was observed which ultimately reached higher values than the ones obtained in germ tube forming cells. Substances which affect calcium metabolism in other cells such as cobalt ions, ionophore A23187 and compound R24571 were observed to inhibit germ tube formation and calcium uptake. In addition, ionophore A23187 was found to increase calcium efflux to approximately twice the control values. The inhibition of germ tube formation brought about by substances which inhibit calcium uptake or increase efflux suggests that the intracellular calcium concentration in these cells must be precisely regulated for the yeast to mycelium transition to occur.

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Effects of divalent cations and functionally related substances on the yeast to mycelium transition inSporothrix schenckii

Cited by 23 publications

References 11 publications

Calcium involvement in dimorphism of Ophiostoma ulmi, the Dutch elm disease fungus, and characterization of calcium uptake by yeast cells and germ tubes

Calcium involvement in dimorphism of Ophiostoma ulmi, the Dutch elm disease fungus, and characterization of calcium uptake by yeast cells and germ tubes

Influence of Zn2+ on yeast-mycelium dimorphism and exopolysaccharide production by the fungus Aureobasidium pullulans grown in a defined medium in continuous culture

Calcium uptake and efflux during the yeast to mycelium transition inSporothrix schenckii

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