Effects of Direct Hemoperfusion with a β₂-Microglobulin Adsorption Column on Hypercytokinemia in Rats

Abstract

Background/Aim: Blood purification therapies have been clinically applied to treat the cytokine-induced pathological responses. The effects of direct hemoperfusion(DHP) with a β₂-microglobulin (BMG) adsorption column on hypercytokinemia were investigated. Methods: A hypercytokinemia model was prepared by injecting lipopolysaccharide (LPS) into femoral veins of male Wistar rats weighing 250–400 g. The hypercytokinemic rats were connected to the DHP system using a small BMG adsorption column, and time course changes in plasma levels of inflammatory cytokines were then examined. Results: Plasma levels of interleukins (IL) 1β and 6 and tumor necrosis factor alpha (TNF-α) were found to be significantly elevated within 3 h after LPS injection in the control group that underwent extracorporeal circulation without the BMG adsorption column, whereas the increases in plasma levels of IL-6 and TNF-α were significantly inhibited in the group that was treated with the BMG adsorption column. The adsorption rates of cytokines with the BMG adsorption column were 20–40% for IL-1β and IL-6 and 10–85% for TNF-α during treatment for 3 h. Conclusions: These results suggest that the systemic inflammatory response syndrome with hypercytokinemia might be treated with DHP using the BMG adsorption column.

Comparison of Free Light Chain Removal by Four Blood Purification Methods

“…LixelleS‐35 is a β 2 MG adsorption column that consists of porous cellulose beads (18), has a ligand of the hexadecyl group on the surface, and is able to adsorb β 2 MG by hydrophobic interaction. It also adsorbs various cytokines (19). In healthy subjects, the concentration of kappa FLC ranges from 3.3 to 19.4 mg/L and that of lambda FLC ranges from 5.7 to 26.3 mg/L.…”

Blood Purification for Critical Illness: Cytokines Adsorption Therapy

“…TNF‐α concentrations were 3270 ± 657 and 6198 ± 640 pg/mL 60 min after LPS injection, 5831 ± 494 and 9037 ± 317 pg/mL 90 min after LPS injection, 5902 ± 463 and 8127 ± 374 pg/mL 120 min after LPS injection, and 3613 ± 254 and 5408 ± 317 pg/mL 150 min after LPS injection in the adsorbent and control groups, respectively. Thus, the increase in TNF‐α concentration was significantly inhibited in the adsorbed group at the time points indicated ( P < 0.05) (16).…”

“…Data are expressed as the mean ± standard error and analyzed using one‐way anova . A P ‐value less than 0.05 was considered significant by Fisher's PLSD test (16).…”